Speculation on the possibility for introducing Anopheles stephensi as a species complex: preliminary evidence based on odorant binding protein 1 intron I sequence
Abstract Background Anopheles stephensi is considered an important malaria vector in Iran, Asia, and recently in the Horn of Africa. Recently, Ansteobp1 intron I sequence has been introduced a new molecular marker for identification of its biological forms including, mysorensis, intermediate and typ...
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ftdoajarticles:oai:doaj.org/article:ed930d02573b420ba750f17c3b24bed3 2023-05-15T15:10:26+02:00 Speculation on the possibility for introducing Anopheles stephensi as a species complex: preliminary evidence based on odorant binding protein 1 intron I sequence Samira Firooziyan Navid Dinparast Djadid Saber Gholizadeh 2018-10-01T00:00:00Z https://doi.org/10.1186/s12936-018-2523-y https://doaj.org/article/ed930d02573b420ba750f17c3b24bed3 EN eng BMC http://link.springer.com/article/10.1186/s12936-018-2523-y https://doaj.org/toc/1475-2875 doi:10.1186/s12936-018-2523-y 1475-2875 https://doaj.org/article/ed930d02573b420ba750f17c3b24bed3 Malaria Journal, Vol 17, Iss 1, Pp 1-7 (2018) Anopheles stephensi complex species An. stephensi sibling A An. stephensi sibling B An. stephensi sibling C Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2018 ftdoajarticles https://doi.org/10.1186/s12936-018-2523-y 2022-12-30T21:07:43Z Abstract Background Anopheles stephensi is considered an important malaria vector in Iran, Asia, and recently in the Horn of Africa. Recently, Ansteobp1 intron I sequence has been introduced a new molecular marker for identification of its biological forms including, mysorensis, intermediate and type, using insectary colony specimens. Methods In the current study, new marker ability in molecular identification of biological forms has been evaluated with An. stephensi specimens collected from Iran and Afghanistan malarious provinces. Following DNA extraction and PCR amplification, sequence analysis and constructed phylogenetic tree revealed that type and intermediate forms are distributed in Iran. Results The specimens collected from Afghanistan identified as intermediate and mysorensis forms. Therefore, intermediate form is sympatric species in both countries. Based on the results of Ansteobp1 intron I sequences, An. stephensi could be suggested as new Anopheles complex species including An. stephensi sibling A (type form), An. stephensi sibling B (intermediate form) and An. stephensi sibling C (mysorensis form). This is the first report on the presence of An. stephensi biological forms in Afghanistan. Conclusions Iran is going to eliminate malaria transmission from the country, precise species identification, especially in complex species will be helpful in the prevention of malaria resurgence in the country, mainly because of common fauna of Anopheles species and through border malaria and population movement within Afghanistan, Pakistan, and Iran. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 17 1 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
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Anopheles stephensi complex species An. stephensi sibling A An. stephensi sibling B An. stephensi sibling C Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Anopheles stephensi complex species An. stephensi sibling A An. stephensi sibling B An. stephensi sibling C Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Samira Firooziyan Navid Dinparast Djadid Saber Gholizadeh Speculation on the possibility for introducing Anopheles stephensi as a species complex: preliminary evidence based on odorant binding protein 1 intron I sequence |
topic_facet |
Anopheles stephensi complex species An. stephensi sibling A An. stephensi sibling B An. stephensi sibling C Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Anopheles stephensi is considered an important malaria vector in Iran, Asia, and recently in the Horn of Africa. Recently, Ansteobp1 intron I sequence has been introduced a new molecular marker for identification of its biological forms including, mysorensis, intermediate and type, using insectary colony specimens. Methods In the current study, new marker ability in molecular identification of biological forms has been evaluated with An. stephensi specimens collected from Iran and Afghanistan malarious provinces. Following DNA extraction and PCR amplification, sequence analysis and constructed phylogenetic tree revealed that type and intermediate forms are distributed in Iran. Results The specimens collected from Afghanistan identified as intermediate and mysorensis forms. Therefore, intermediate form is sympatric species in both countries. Based on the results of Ansteobp1 intron I sequences, An. stephensi could be suggested as new Anopheles complex species including An. stephensi sibling A (type form), An. stephensi sibling B (intermediate form) and An. stephensi sibling C (mysorensis form). This is the first report on the presence of An. stephensi biological forms in Afghanistan. Conclusions Iran is going to eliminate malaria transmission from the country, precise species identification, especially in complex species will be helpful in the prevention of malaria resurgence in the country, mainly because of common fauna of Anopheles species and through border malaria and population movement within Afghanistan, Pakistan, and Iran. |
format |
Article in Journal/Newspaper |
author |
Samira Firooziyan Navid Dinparast Djadid Saber Gholizadeh |
author_facet |
Samira Firooziyan Navid Dinparast Djadid Saber Gholizadeh |
author_sort |
Samira Firooziyan |
title |
Speculation on the possibility for introducing Anopheles stephensi as a species complex: preliminary evidence based on odorant binding protein 1 intron I sequence |
title_short |
Speculation on the possibility for introducing Anopheles stephensi as a species complex: preliminary evidence based on odorant binding protein 1 intron I sequence |
title_full |
Speculation on the possibility for introducing Anopheles stephensi as a species complex: preliminary evidence based on odorant binding protein 1 intron I sequence |
title_fullStr |
Speculation on the possibility for introducing Anopheles stephensi as a species complex: preliminary evidence based on odorant binding protein 1 intron I sequence |
title_full_unstemmed |
Speculation on the possibility for introducing Anopheles stephensi as a species complex: preliminary evidence based on odorant binding protein 1 intron I sequence |
title_sort |
speculation on the possibility for introducing anopheles stephensi as a species complex: preliminary evidence based on odorant binding protein 1 intron i sequence |
publisher |
BMC |
publishDate |
2018 |
url |
https://doi.org/10.1186/s12936-018-2523-y https://doaj.org/article/ed930d02573b420ba750f17c3b24bed3 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 17, Iss 1, Pp 1-7 (2018) |
op_relation |
http://link.springer.com/article/10.1186/s12936-018-2523-y https://doaj.org/toc/1475-2875 doi:10.1186/s12936-018-2523-y 1475-2875 https://doaj.org/article/ed930d02573b420ba750f17c3b24bed3 |
op_doi |
https://doi.org/10.1186/s12936-018-2523-y |
container_title |
Malaria Journal |
container_volume |
17 |
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1 |
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1766341461987557376 |