Respiratory cryptosporidiosis in Malawian children with diarrheal disease.
Background Respiratory cryptosporidiosis has been documented in children with diarrhea. We sought to describe the dynamics of respiratory involvement in children hospitalized with gastrointestinal (GI) diarrheal disease. Methods We conducted a prospective, observational longitudinal study of Malawia...
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ftdoajarticles:oai:doaj.org/article:ed75b0027c3c4302ac0cb4eb9b2d2370 2023-05-15T15:12:36+02:00 Respiratory cryptosporidiosis in Malawian children with diarrheal disease. Pui-Ying Iroh Tam Mphatso Chisala Wongani Nyangulu Herbert Thole James Nyirenda 2021-07-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0009643 https://doaj.org/article/ed75b0027c3c4302ac0cb4eb9b2d2370 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0009643 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009643 https://doaj.org/article/ed75b0027c3c4302ac0cb4eb9b2d2370 PLoS Neglected Tropical Diseases, Vol 15, Iss 7, p e0009643 (2021) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2021 ftdoajarticles https://doi.org/10.1371/journal.pntd.0009643 2022-12-31T15:49:17Z Background Respiratory cryptosporidiosis has been documented in children with diarrhea. We sought to describe the dynamics of respiratory involvement in children hospitalized with gastrointestinal (GI) diarrheal disease. Methods We conducted a prospective, observational longitudinal study of Malawian children 2-24 months hospitalized with diarrhea. Nasopharyngeal (NP) swabs, induced sputum and stool specimens were collected. Participants that were positive by Cryptosporidium PCR in any of the three compartments were followed up with fortnightly visits up to 8 weeks post-enrollment. Results Of the 162 children recruited, participants had mild-moderate malnutrition (mean HAZ -1.6 (SD 2.1)), 37 (21%) were PCR-positive for Cryptosporidium at enrollment (37 stool, 11 sputum, and 4 NP) and 27 completed the majority of follow-up visits (73%). Cryptosporidium was detected in all compartments over the 4 post-enrollment visits, most commonly in stool (100% at enrollment with mean cycle thresholds (Ct) of 28.8±4.3 to 44% at 8 weeks with Ct 29.9±4.1), followed by sputum (31% at enrollment with mean Ct 31.1±4.4 to 20% at 8 weeks with Ct 35.7±2.6), then NP (11% with mean Ct 33.5±1.0 to 8% with Ct 36.6±0.7). Participants with Cryptosporidium detection in both the respiratory and GI tract over the study period reported respiratory and GI symptoms in 81% and 62% of study visits, respectively, compared to 68% and 27%, respectively, for those with only GI detection, and had longer GI shedding (17.5±6.6 v. 15.9±2.9 days). Conclusion Cryptosporidium was detected in both respiratory and GI tracts throughout the 8 weeks post-enrollment. The development of therapeutics for Cryptosporidium in children should target the respiratory as well as GI tract. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 15 7 e0009643 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Pui-Ying Iroh Tam Mphatso Chisala Wongani Nyangulu Herbert Thole James Nyirenda Respiratory cryptosporidiosis in Malawian children with diarrheal disease. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Background Respiratory cryptosporidiosis has been documented in children with diarrhea. We sought to describe the dynamics of respiratory involvement in children hospitalized with gastrointestinal (GI) diarrheal disease. Methods We conducted a prospective, observational longitudinal study of Malawian children 2-24 months hospitalized with diarrhea. Nasopharyngeal (NP) swabs, induced sputum and stool specimens were collected. Participants that were positive by Cryptosporidium PCR in any of the three compartments were followed up with fortnightly visits up to 8 weeks post-enrollment. Results Of the 162 children recruited, participants had mild-moderate malnutrition (mean HAZ -1.6 (SD 2.1)), 37 (21%) were PCR-positive for Cryptosporidium at enrollment (37 stool, 11 sputum, and 4 NP) and 27 completed the majority of follow-up visits (73%). Cryptosporidium was detected in all compartments over the 4 post-enrollment visits, most commonly in stool (100% at enrollment with mean cycle thresholds (Ct) of 28.8±4.3 to 44% at 8 weeks with Ct 29.9±4.1), followed by sputum (31% at enrollment with mean Ct 31.1±4.4 to 20% at 8 weeks with Ct 35.7±2.6), then NP (11% with mean Ct 33.5±1.0 to 8% with Ct 36.6±0.7). Participants with Cryptosporidium detection in both the respiratory and GI tract over the study period reported respiratory and GI symptoms in 81% and 62% of study visits, respectively, compared to 68% and 27%, respectively, for those with only GI detection, and had longer GI shedding (17.5±6.6 v. 15.9±2.9 days). Conclusion Cryptosporidium was detected in both respiratory and GI tracts throughout the 8 weeks post-enrollment. The development of therapeutics for Cryptosporidium in children should target the respiratory as well as GI tract. |
format |
Article in Journal/Newspaper |
author |
Pui-Ying Iroh Tam Mphatso Chisala Wongani Nyangulu Herbert Thole James Nyirenda |
author_facet |
Pui-Ying Iroh Tam Mphatso Chisala Wongani Nyangulu Herbert Thole James Nyirenda |
author_sort |
Pui-Ying Iroh Tam |
title |
Respiratory cryptosporidiosis in Malawian children with diarrheal disease. |
title_short |
Respiratory cryptosporidiosis in Malawian children with diarrheal disease. |
title_full |
Respiratory cryptosporidiosis in Malawian children with diarrheal disease. |
title_fullStr |
Respiratory cryptosporidiosis in Malawian children with diarrheal disease. |
title_full_unstemmed |
Respiratory cryptosporidiosis in Malawian children with diarrheal disease. |
title_sort |
respiratory cryptosporidiosis in malawian children with diarrheal disease. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2021 |
url |
https://doi.org/10.1371/journal.pntd.0009643 https://doaj.org/article/ed75b0027c3c4302ac0cb4eb9b2d2370 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 15, Iss 7, p e0009643 (2021) |
op_relation |
https://doi.org/10.1371/journal.pntd.0009643 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009643 https://doaj.org/article/ed75b0027c3c4302ac0cb4eb9b2d2370 |
op_doi |
https://doi.org/10.1371/journal.pntd.0009643 |
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PLOS Neglected Tropical Diseases |
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15 |
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7 |
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e0009643 |
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1766343266177908736 |