Genetic characterization of Trypanosoma cruzi DTUs in wild Triatoma infestans from Bolivia: predominance of TcI.
BACKGROUND: The current persistence of Triatoma infestans (one of the main vectors of Chagas disease) in some domestic areas could be related to re-colonization by wild populations which are increasingly reported. However, the infection rate and the genetic characterization of the Trypanosoma cruzi...
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ftdoajarticles:oai:doaj.org/article:ed731214a387412bb48734b521e8e300 2023-05-15T15:07:49+02:00 Genetic characterization of Trypanosoma cruzi DTUs in wild Triatoma infestans from Bolivia: predominance of TcI. Simone Frédérique Brenière Claudia Aliaga Etienne Waleckx Rosio Buitrago Renata Salas Christian Barnabé Michel Tibayrenc François Noireau 2012-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0001650 https://doaj.org/article/ed731214a387412bb48734b521e8e300 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3368956?pdf=render https://doaj.org/toc/1935-2735 1935-2735 doi:10.1371/journal.pntd.0001650 https://doaj.org/article/ed731214a387412bb48734b521e8e300 PLoS Neglected Tropical Diseases, Vol 6, Iss 5, p e1650 (2012) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2012 ftdoajarticles https://doi.org/10.1371/journal.pntd.0001650 2022-12-31T12:24:06Z BACKGROUND: The current persistence of Triatoma infestans (one of the main vectors of Chagas disease) in some domestic areas could be related to re-colonization by wild populations which are increasingly reported. However, the infection rate and the genetic characterization of the Trypanosoma cruzi strains infecting these populations are very limited. METHODOLOGY/PRINCIPAL FINDINGS: Of 333 wild Triatoma infestans specimens collected from north to south of a Chagas disease endemic area in Bolivia, we characterized 234 stocks of Trypanosoma cruzi using mini-exon multiplex PCR (MMPCR) and sequencing the glucose phosphate isomerase (Gpi) gene. Of the six genetic lineages ("discrete typing units"; DTU) (TcI-VI) presently recognized in T. cruzi, TcI (99.1%) was overdominant on TcIII (0.9%) in wild Andean T. infestans, which presented a 71.7% infection rate as evaluated by microscopy. In the lowlands (Bolivian Chaco), 17 "dark morph" T. infestans were analyzed. None of them were positive for parasites after microscopic examination, although one TcI stock and one TcII stock were identified using MMPCR and sequencing. CONCLUSIONS/SIGNIFICANCE: By exploring large-scale DTUs that infect the wild populations of T. infestans, this study opens the discussion on the origin of TcI and TcV DTUs that are predominant in domestic Bolivian cycles. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Chaco ENVELOPE(-60.583,-60.583,-63.033,-63.033) PLoS Neglected Tropical Diseases 6 5 e1650 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Simone Frédérique Brenière Claudia Aliaga Etienne Waleckx Rosio Buitrago Renata Salas Christian Barnabé Michel Tibayrenc François Noireau Genetic characterization of Trypanosoma cruzi DTUs in wild Triatoma infestans from Bolivia: predominance of TcI. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND: The current persistence of Triatoma infestans (one of the main vectors of Chagas disease) in some domestic areas could be related to re-colonization by wild populations which are increasingly reported. However, the infection rate and the genetic characterization of the Trypanosoma cruzi strains infecting these populations are very limited. METHODOLOGY/PRINCIPAL FINDINGS: Of 333 wild Triatoma infestans specimens collected from north to south of a Chagas disease endemic area in Bolivia, we characterized 234 stocks of Trypanosoma cruzi using mini-exon multiplex PCR (MMPCR) and sequencing the glucose phosphate isomerase (Gpi) gene. Of the six genetic lineages ("discrete typing units"; DTU) (TcI-VI) presently recognized in T. cruzi, TcI (99.1%) was overdominant on TcIII (0.9%) in wild Andean T. infestans, which presented a 71.7% infection rate as evaluated by microscopy. In the lowlands (Bolivian Chaco), 17 "dark morph" T. infestans were analyzed. None of them were positive for parasites after microscopic examination, although one TcI stock and one TcII stock were identified using MMPCR and sequencing. CONCLUSIONS/SIGNIFICANCE: By exploring large-scale DTUs that infect the wild populations of T. infestans, this study opens the discussion on the origin of TcI and TcV DTUs that are predominant in domestic Bolivian cycles. |
format |
Article in Journal/Newspaper |
author |
Simone Frédérique Brenière Claudia Aliaga Etienne Waleckx Rosio Buitrago Renata Salas Christian Barnabé Michel Tibayrenc François Noireau |
author_facet |
Simone Frédérique Brenière Claudia Aliaga Etienne Waleckx Rosio Buitrago Renata Salas Christian Barnabé Michel Tibayrenc François Noireau |
author_sort |
Simone Frédérique Brenière |
title |
Genetic characterization of Trypanosoma cruzi DTUs in wild Triatoma infestans from Bolivia: predominance of TcI. |
title_short |
Genetic characterization of Trypanosoma cruzi DTUs in wild Triatoma infestans from Bolivia: predominance of TcI. |
title_full |
Genetic characterization of Trypanosoma cruzi DTUs in wild Triatoma infestans from Bolivia: predominance of TcI. |
title_fullStr |
Genetic characterization of Trypanosoma cruzi DTUs in wild Triatoma infestans from Bolivia: predominance of TcI. |
title_full_unstemmed |
Genetic characterization of Trypanosoma cruzi DTUs in wild Triatoma infestans from Bolivia: predominance of TcI. |
title_sort |
genetic characterization of trypanosoma cruzi dtus in wild triatoma infestans from bolivia: predominance of tci. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2012 |
url |
https://doi.org/10.1371/journal.pntd.0001650 https://doaj.org/article/ed731214a387412bb48734b521e8e300 |
long_lat |
ENVELOPE(-60.583,-60.583,-63.033,-63.033) |
geographic |
Arctic Chaco |
geographic_facet |
Arctic Chaco |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 6, Iss 5, p e1650 (2012) |
op_relation |
http://europepmc.org/articles/PMC3368956?pdf=render https://doaj.org/toc/1935-2735 1935-2735 doi:10.1371/journal.pntd.0001650 https://doaj.org/article/ed731214a387412bb48734b521e8e300 |
op_doi |
https://doi.org/10.1371/journal.pntd.0001650 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
6 |
container_issue |
5 |
container_start_page |
e1650 |
_version_ |
1766339234390605824 |