Antimycobacterial and cytotoxicity activity of microcystins
Background The present work aimed to evaluate the antimycobacterial activity and cytotoxicity of Microcystis aeruginosa toxins, the MC-LR variant and purified extract of [D-Leu1] microcystin-LR. Methods The antimicrobial activity of M. aeruginosa extract and microcystin was evaluated by resazurin mi...
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ftdoajarticles:oai:doaj.org/article:ed71031f8206463fb40b114fd636bff0 2023-05-15T15:06:37+02:00 Antimycobacterial and cytotoxicity activity of microcystins Daniela Fernandes Ramos Alexandre Matthiensen Wilson Colvara Ana Paula Souza de Votto Gilma Santos Trindade Pedro Eduardo Almeida da Silva João Sarkis Yunes 2015-03-01T00:00:00Z https://doi.org/10.1186/s40409-015-0009-8 https://doaj.org/article/ed71031f8206463fb40b114fd636bff0 EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100315&lng=en&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1186/s40409-015-0009-8 https://doaj.org/article/ed71031f8206463fb40b114fd636bff0 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 21, Iss 0 (2015) Mycobacteria Antimycobacterial agents Cytotoxic activity Microcystins Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2015 ftdoajarticles https://doi.org/10.1186/s40409-015-0009-8 2022-12-31T16:07:54Z Background The present work aimed to evaluate the antimycobacterial activity and cytotoxicity of Microcystis aeruginosa toxins, the MC-LR variant and purified extract of [D-Leu1] microcystin-LR. Methods The antimicrobial activity of M. aeruginosa extract and microcystin was evaluated by resazurin microtiter assay against Mycobacterium tuberculosis, M. terrae, M. chelonae and M. kansasii. The cytotoxicity assay was performed by trypan blue exclusion against the HTC cell line. Results Antimicrobial activity was observed in the hexanic extract of M. aeruginosa (RST 9501 strain) against M. tuberculosis, including sensitive and resistant strains with minimal inhibitory concentrations (MIC) between 1.93 μM and 0.06 μM. The high activity of M. aeruginosa hexanic extract could be attributed to the major presence of the toxins MC-LR and [D-Leu1] MC-LR that showed activity at MIC between 53 and 0.42 μM against tested mycobacterial strains. Even at the highest concentration tested, no toxicity of M. aeruginosa extracts was identified against HTC cells. Conclusions These preliminary results suggest that [D-Leu1] MC-LR is a promising candidate for the development of a new antimycobacterial agent. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 21 1 9 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Mycobacteria Antimycobacterial agents Cytotoxic activity Microcystins Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
Mycobacteria Antimycobacterial agents Cytotoxic activity Microcystins Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 Daniela Fernandes Ramos Alexandre Matthiensen Wilson Colvara Ana Paula Souza de Votto Gilma Santos Trindade Pedro Eduardo Almeida da Silva João Sarkis Yunes Antimycobacterial and cytotoxicity activity of microcystins |
topic_facet |
Mycobacteria Antimycobacterial agents Cytotoxic activity Microcystins Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Background The present work aimed to evaluate the antimycobacterial activity and cytotoxicity of Microcystis aeruginosa toxins, the MC-LR variant and purified extract of [D-Leu1] microcystin-LR. Methods The antimicrobial activity of M. aeruginosa extract and microcystin was evaluated by resazurin microtiter assay against Mycobacterium tuberculosis, M. terrae, M. chelonae and M. kansasii. The cytotoxicity assay was performed by trypan blue exclusion against the HTC cell line. Results Antimicrobial activity was observed in the hexanic extract of M. aeruginosa (RST 9501 strain) against M. tuberculosis, including sensitive and resistant strains with minimal inhibitory concentrations (MIC) between 1.93 μM and 0.06 μM. The high activity of M. aeruginosa hexanic extract could be attributed to the major presence of the toxins MC-LR and [D-Leu1] MC-LR that showed activity at MIC between 53 and 0.42 μM against tested mycobacterial strains. Even at the highest concentration tested, no toxicity of M. aeruginosa extracts was identified against HTC cells. Conclusions These preliminary results suggest that [D-Leu1] MC-LR is a promising candidate for the development of a new antimycobacterial agent. |
format |
Article in Journal/Newspaper |
author |
Daniela Fernandes Ramos Alexandre Matthiensen Wilson Colvara Ana Paula Souza de Votto Gilma Santos Trindade Pedro Eduardo Almeida da Silva João Sarkis Yunes |
author_facet |
Daniela Fernandes Ramos Alexandre Matthiensen Wilson Colvara Ana Paula Souza de Votto Gilma Santos Trindade Pedro Eduardo Almeida da Silva João Sarkis Yunes |
author_sort |
Daniela Fernandes Ramos |
title |
Antimycobacterial and cytotoxicity activity of microcystins |
title_short |
Antimycobacterial and cytotoxicity activity of microcystins |
title_full |
Antimycobacterial and cytotoxicity activity of microcystins |
title_fullStr |
Antimycobacterial and cytotoxicity activity of microcystins |
title_full_unstemmed |
Antimycobacterial and cytotoxicity activity of microcystins |
title_sort |
antimycobacterial and cytotoxicity activity of microcystins |
publisher |
SciELO |
publishDate |
2015 |
url |
https://doi.org/10.1186/s40409-015-0009-8 https://doaj.org/article/ed71031f8206463fb40b114fd636bff0 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 21, Iss 0 (2015) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100315&lng=en&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1186/s40409-015-0009-8 https://doaj.org/article/ed71031f8206463fb40b114fd636bff0 |
op_doi |
https://doi.org/10.1186/s40409-015-0009-8 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
21 |
container_issue |
1 |
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9 |
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