Genetic variants of RNASE3 (ECP) and susceptibility to severe malaria in Senegalese population

Abstract Background Severe forms of malaria (SM) are an outcome of Plasmodium falciparum infection and can cause death especially in children under 4 years of age. RNASE3 (ECP) has been identified as an inhibitor of Plasmodium parasites growth in vitro, and genetic analysis in hospitalized Ghanaian...

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Published in:Malaria Journal
Main Authors: Gora Diop, Céline Derbois, Cheikh Loucoubar, Babacar Mbengue, Bineta Niakhana Ndao, Fatou Thiam, Alassane Thiam, Rokhaya Ndiaye, Yakhya Dieye, Robert Olaso, Jean-Francois Deleuze, Alioune Dieye
Format: Article in Journal/Newspaper
Language:English
Published: BMC 2018
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Online Access:https://doi.org/10.1186/s12936-018-2205-9
https://doaj.org/article/ed5cfb85490a483683c263f98fba2dbd
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spelling ftdoajarticles:oai:doaj.org/article:ed5cfb85490a483683c263f98fba2dbd 2023-05-15T15:16:34+02:00 Genetic variants of RNASE3 (ECP) and susceptibility to severe malaria in Senegalese population Gora Diop Céline Derbois Cheikh Loucoubar Babacar Mbengue Bineta Niakhana Ndao Fatou Thiam Alassane Thiam Rokhaya Ndiaye Yakhya Dieye Robert Olaso Jean-Francois Deleuze Alioune Dieye 2018-02-01T00:00:00Z https://doi.org/10.1186/s12936-018-2205-9 https://doaj.org/article/ed5cfb85490a483683c263f98fba2dbd EN eng BMC http://link.springer.com/article/10.1186/s12936-018-2205-9 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-018-2205-9 1475-2875 https://doaj.org/article/ed5cfb85490a483683c263f98fba2dbd Malaria Journal, Vol 17, Iss 1, Pp 1-10 (2018) Severe malaria Plasmodium falciparum Susceptibility RNase3 (ECP) gene Polymorphisms Senegal Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2018 ftdoajarticles https://doi.org/10.1186/s12936-018-2205-9 2022-12-31T01:57:39Z Abstract Background Severe forms of malaria (SM) are an outcome of Plasmodium falciparum infection and can cause death especially in children under 4 years of age. RNASE3 (ECP) has been identified as an inhibitor of Plasmodium parasites growth in vitro, and genetic analysis in hospitalized Ghanaian subjects has revealed the RNASE3 +371G/C (rs2073342) polymorphism as a susceptibility factor for cerebral malaria. The +371 C allele results in an Arg/Thr mutation that abolishes the cytotoxic activity of the ECP protein. The present study aims to investigate RNASE3 gene polymorphisms and their putative link to severe malaria in a malaria cohort from Senegal. Methods/results Patients enrolled from hospitals were classified as having either uncomplicated (UM) or severe malaria (SM). The analysis of the RNASE3 gene polymorphisms was performed in 241 subjects: 178 falciparum infected (96 SM, 82 UM) and 63 non-infected subjects as population control group (CTR). Six frequent SNPs (MAF > 3%) were identified, and one SNP was associated with malaria severity by performing a logistic regression analysis SM vs.UM: RNASE3 +499G/C (rs2233860) under age, sex as covariates and HbS/HbC polymorphisms adjustment (p = 0.003, OR 0.43, CI 95% 0.20–0.92). The polymorphisms: +371G/C (rs2073342), +499G/C (rs2233860) and +577A/T (rs8019343) defined a haplotype risk (G-G-T) for malaria severity (Fisher exact test, p = 0.03) (OR 4.1, IC 95% (1.1–14.9). Conclusion In addition to the previously described association of +371G/C polymorphism in Ghanaians cohort, the RNASE3 +499G/C polymorphism was associated with susceptibility to SM in a Senegalese population. The haplotype +371G/+499G/+577T defined by RNASE3 polymorphisms was associated with severity. The genetic association identified independently in the Senegalese population provide additional evidence of a role of RNASE3 (ECP) in malaria severity. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 17 1
institution Open Polar
collection Directory of Open Access Journals: DOAJ Articles
op_collection_id ftdoajarticles
language English
topic Severe malaria
Plasmodium falciparum
Susceptibility
RNase3 (ECP) gene
Polymorphisms
Senegal
Arctic medicine. Tropical medicine
RC955-962
Infectious and parasitic diseases
RC109-216
spellingShingle Severe malaria
Plasmodium falciparum
Susceptibility
RNase3 (ECP) gene
Polymorphisms
Senegal
Arctic medicine. Tropical medicine
RC955-962
Infectious and parasitic diseases
RC109-216
Gora Diop
Céline Derbois
Cheikh Loucoubar
Babacar Mbengue
Bineta Niakhana Ndao
Fatou Thiam
Alassane Thiam
Rokhaya Ndiaye
Yakhya Dieye
Robert Olaso
Jean-Francois Deleuze
Alioune Dieye
Genetic variants of RNASE3 (ECP) and susceptibility to severe malaria in Senegalese population
topic_facet Severe malaria
Plasmodium falciparum
Susceptibility
RNase3 (ECP) gene
Polymorphisms
Senegal
Arctic medicine. Tropical medicine
RC955-962
Infectious and parasitic diseases
RC109-216
description Abstract Background Severe forms of malaria (SM) are an outcome of Plasmodium falciparum infection and can cause death especially in children under 4 years of age. RNASE3 (ECP) has been identified as an inhibitor of Plasmodium parasites growth in vitro, and genetic analysis in hospitalized Ghanaian subjects has revealed the RNASE3 +371G/C (rs2073342) polymorphism as a susceptibility factor for cerebral malaria. The +371 C allele results in an Arg/Thr mutation that abolishes the cytotoxic activity of the ECP protein. The present study aims to investigate RNASE3 gene polymorphisms and their putative link to severe malaria in a malaria cohort from Senegal. Methods/results Patients enrolled from hospitals were classified as having either uncomplicated (UM) or severe malaria (SM). The analysis of the RNASE3 gene polymorphisms was performed in 241 subjects: 178 falciparum infected (96 SM, 82 UM) and 63 non-infected subjects as population control group (CTR). Six frequent SNPs (MAF > 3%) were identified, and one SNP was associated with malaria severity by performing a logistic regression analysis SM vs.UM: RNASE3 +499G/C (rs2233860) under age, sex as covariates and HbS/HbC polymorphisms adjustment (p = 0.003, OR 0.43, CI 95% 0.20–0.92). The polymorphisms: +371G/C (rs2073342), +499G/C (rs2233860) and +577A/T (rs8019343) defined a haplotype risk (G-G-T) for malaria severity (Fisher exact test, p = 0.03) (OR 4.1, IC 95% (1.1–14.9). Conclusion In addition to the previously described association of +371G/C polymorphism in Ghanaians cohort, the RNASE3 +499G/C polymorphism was associated with susceptibility to SM in a Senegalese population. The haplotype +371G/+499G/+577T defined by RNASE3 polymorphisms was associated with severity. The genetic association identified independently in the Senegalese population provide additional evidence of a role of RNASE3 (ECP) in malaria severity.
format Article in Journal/Newspaper
author Gora Diop
Céline Derbois
Cheikh Loucoubar
Babacar Mbengue
Bineta Niakhana Ndao
Fatou Thiam
Alassane Thiam
Rokhaya Ndiaye
Yakhya Dieye
Robert Olaso
Jean-Francois Deleuze
Alioune Dieye
author_facet Gora Diop
Céline Derbois
Cheikh Loucoubar
Babacar Mbengue
Bineta Niakhana Ndao
Fatou Thiam
Alassane Thiam
Rokhaya Ndiaye
Yakhya Dieye
Robert Olaso
Jean-Francois Deleuze
Alioune Dieye
author_sort Gora Diop
title Genetic variants of RNASE3 (ECP) and susceptibility to severe malaria in Senegalese population
title_short Genetic variants of RNASE3 (ECP) and susceptibility to severe malaria in Senegalese population
title_full Genetic variants of RNASE3 (ECP) and susceptibility to severe malaria in Senegalese population
title_fullStr Genetic variants of RNASE3 (ECP) and susceptibility to severe malaria in Senegalese population
title_full_unstemmed Genetic variants of RNASE3 (ECP) and susceptibility to severe malaria in Senegalese population
title_sort genetic variants of rnase3 (ecp) and susceptibility to severe malaria in senegalese population
publisher BMC
publishDate 2018
url https://doi.org/10.1186/s12936-018-2205-9
https://doaj.org/article/ed5cfb85490a483683c263f98fba2dbd
geographic Arctic
geographic_facet Arctic
genre Arctic
genre_facet Arctic
op_source Malaria Journal, Vol 17, Iss 1, Pp 1-10 (2018)
op_relation http://link.springer.com/article/10.1186/s12936-018-2205-9
https://doaj.org/toc/1475-2875
doi:10.1186/s12936-018-2205-9
1475-2875
https://doaj.org/article/ed5cfb85490a483683c263f98fba2dbd
op_doi https://doi.org/10.1186/s12936-018-2205-9
container_title Malaria Journal
container_volume 17
container_issue 1
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