Cholera toxin A1 residues single alanine substitutional mutation and effect on activity with stimulatory G protein
Cholera is a well-known gastrointestinal infection. The cholera toxin is an important pathological substance in pathogenesis of cholera diarrhea. Cholera toxin is composed of catalytic A1 subunit, an A2 linker, and a homopentameric cell-binding B subunit. In enterocyte, cholera toxin will attach to...
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Wolters Kluwer Medknow Publications
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ftdoajarticles:oai:doaj.org/article:ed53de9a619949738678780b088dba47 2023-05-15T15:08:18+02:00 Cholera toxin A1 residues single alanine substitutional mutation and effect on activity with stimulatory G protein Somsri Wiwanitkit Viroj Wiwanitkit 2017-06-01T00:00:00Z https://doi.org/10.1016/j.apjtb.2017.05.012 https://doaj.org/article/ed53de9a619949738678780b088dba47 EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169116300235 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2017.05.012 https://doaj.org/article/ed53de9a619949738678780b088dba47 Asian Pacific Journal of Tropical Biomedicine, Vol 7, Iss 6, Pp 585-586 (2017) Cholera Toxin Mutation Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2017 ftdoajarticles https://doi.org/10.1016/j.apjtb.2017.05.012 2022-12-31T14:14:59Z Cholera is a well-known gastrointestinal infection. The cholera toxin is an important pathological substance in pathogenesis of cholera diarrhea. Cholera toxin is composed of catalytic A1 subunit, an A2 linker, and a homopentameric cell-binding B subunit. In enterocyte, cholera toxin will attach to GM1 ganglioside receptors on the apical membrane and causes retrograde vesicular trafficking to endoplasmic reticulum. At endoplasmic reticulum, cholera toxin A1 is released from the rest of the toxin into cytoplasm. The cholera toxin A1 interacts will catalyze ADP ribosylation of subunits of stimulatory G protein resulting a persistent activation of adenylate cyclase and an elevation of intracellular cAMP which further result in diarrhea. The single alanine substitutional mutation can result in the reduction of the interaction activity between cholera toxin A1 and stimulatory G protein. In this study, the four well-known mutations, H55, R67, L71, S78, or D109, of cholera toxin A1 is focused. The author hereby calculates for the reaction energy for the reaction between cholera toxin A1 and stimulatory G protein in naïve case and mutated case. To calculate, the standard bonding energy calculation technique in mutation analysis was used. It can be seen that aberrant in reaction energy in each studied mutation is different and can imply the different effect on activity with stimulatory G protein. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 7 6 585 586 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Cholera Toxin Mutation Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
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Cholera Toxin Mutation Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Somsri Wiwanitkit Viroj Wiwanitkit Cholera toxin A1 residues single alanine substitutional mutation and effect on activity with stimulatory G protein |
topic_facet |
Cholera Toxin Mutation Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Cholera is a well-known gastrointestinal infection. The cholera toxin is an important pathological substance in pathogenesis of cholera diarrhea. Cholera toxin is composed of catalytic A1 subunit, an A2 linker, and a homopentameric cell-binding B subunit. In enterocyte, cholera toxin will attach to GM1 ganglioside receptors on the apical membrane and causes retrograde vesicular trafficking to endoplasmic reticulum. At endoplasmic reticulum, cholera toxin A1 is released from the rest of the toxin into cytoplasm. The cholera toxin A1 interacts will catalyze ADP ribosylation of subunits of stimulatory G protein resulting a persistent activation of adenylate cyclase and an elevation of intracellular cAMP which further result in diarrhea. The single alanine substitutional mutation can result in the reduction of the interaction activity between cholera toxin A1 and stimulatory G protein. In this study, the four well-known mutations, H55, R67, L71, S78, or D109, of cholera toxin A1 is focused. The author hereby calculates for the reaction energy for the reaction between cholera toxin A1 and stimulatory G protein in naïve case and mutated case. To calculate, the standard bonding energy calculation technique in mutation analysis was used. It can be seen that aberrant in reaction energy in each studied mutation is different and can imply the different effect on activity with stimulatory G protein. |
format |
Article in Journal/Newspaper |
author |
Somsri Wiwanitkit Viroj Wiwanitkit |
author_facet |
Somsri Wiwanitkit Viroj Wiwanitkit |
author_sort |
Somsri Wiwanitkit |
title |
Cholera toxin A1 residues single alanine substitutional mutation and effect on activity with stimulatory G protein |
title_short |
Cholera toxin A1 residues single alanine substitutional mutation and effect on activity with stimulatory G protein |
title_full |
Cholera toxin A1 residues single alanine substitutional mutation and effect on activity with stimulatory G protein |
title_fullStr |
Cholera toxin A1 residues single alanine substitutional mutation and effect on activity with stimulatory G protein |
title_full_unstemmed |
Cholera toxin A1 residues single alanine substitutional mutation and effect on activity with stimulatory G protein |
title_sort |
cholera toxin a1 residues single alanine substitutional mutation and effect on activity with stimulatory g protein |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2017 |
url |
https://doi.org/10.1016/j.apjtb.2017.05.012 https://doaj.org/article/ed53de9a619949738678780b088dba47 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 7, Iss 6, Pp 585-586 (2017) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2221169116300235 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2017.05.012 https://doaj.org/article/ed53de9a619949738678780b088dba47 |
op_doi |
https://doi.org/10.1016/j.apjtb.2017.05.012 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
7 |
container_issue |
6 |
container_start_page |
585 |
op_container_end_page |
586 |
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1766339682121023488 |