THE SUSCEPTIBILITY OF RECENT ISOLATES OF Schistosoma mansoni TO PRAZIQUANTEL
Introduction: Schistosomiasis is a chronic disease caused by trematode flatworms of the genus Schistosoma and its control is dependent on a single drug, praziquantel (PZQ), but concerns over PZQ resistance have renewed interest in evaluating the in vitro susceptibility of recent isolates of Schistos...
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Universidade de São Paulo (USP)
2016
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ftdoajarticles:oai:doaj.org/article:ed372cac38ab4fac9117e2b98f351d0c 2024-09-09T19:26:26+00:00 THE SUSCEPTIBILITY OF RECENT ISOLATES OF Schistosoma mansoni TO PRAZIQUANTEL Adriana Maria B. MENDONÇA Ana Paula S. FEITOSA Dyana L. VERAS Thiago J. MATOS-ROCHA Marília G. dos Santos CAVALCANTI Constança Clara G. S. BARBOSA Fábio A. BRAYNER Luiz C. ALVES 2016-01-01T00:00:00Z https://doi.org/10.1590/S1678-9946201658007 https://doaj.org/article/ed372cac38ab4fac9117e2b98f351d0c EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652016005000206&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/S1678-9946201658007 https://doaj.org/article/ed372cac38ab4fac9117e2b98f351d0c Revista do Instituto de Medicina Tropical de São Paulo, Vol 58, Iss 0 (2016) Praziquantel Schistosoma mansoni Ultrastructure Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2016 ftdoajarticles https://doi.org/10.1590/S1678-9946201658007 2024-08-05T17:49:31Z Introduction: Schistosomiasis is a chronic disease caused by trematode flatworms of the genus Schistosoma and its control is dependent on a single drug, praziquantel (PZQ), but concerns over PZQ resistance have renewed interest in evaluating the in vitro susceptibility of recent isolates of Schistosoma mansoni to PZQ in comparison with well-established strains in the laboratory. Material and methods: The in vitro activity of PZQ (6.5-0.003 µg/mL) was evaluated in terms of mortality, reduced motor activity and ultrastructural alterations against S. mansoni. Results: After 3 h of incubation, PZQ, at 6.5 µg/mL, caused 100% mortality of all adult worms in the three types of recent isolates, while PZQ was inactive at concentrations of 0.08-0.003 µg/mL after 3 h of incubation. The results show that the SLM and Sotave isolates basically presented the same pattern of susceptibility, differing only in the concentration of 6.5 µg/mL, where deaths occurred from the range of 1.5 h in Sotave and just in the 3 h range of SLM. Additionally, this article presents ultrastructural evidence of rapid severe PZQ-induced surface membrane damage in S. mansoni after treatment with the drug, such as disintegration, sloughing, and erosion of the surface. Conclusion: According to these results, PZQ is very effective to induce tegument destruction of recent isolates of S. mansoni. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista do Instituto de Medicina Tropical de São Paulo 58 0 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
topic |
Praziquantel Schistosoma mansoni Ultrastructure Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Praziquantel Schistosoma mansoni Ultrastructure Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Adriana Maria B. MENDONÇA Ana Paula S. FEITOSA Dyana L. VERAS Thiago J. MATOS-ROCHA Marília G. dos Santos CAVALCANTI Constança Clara G. S. BARBOSA Fábio A. BRAYNER Luiz C. ALVES THE SUSCEPTIBILITY OF RECENT ISOLATES OF Schistosoma mansoni TO PRAZIQUANTEL |
topic_facet |
Praziquantel Schistosoma mansoni Ultrastructure Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Introduction: Schistosomiasis is a chronic disease caused by trematode flatworms of the genus Schistosoma and its control is dependent on a single drug, praziquantel (PZQ), but concerns over PZQ resistance have renewed interest in evaluating the in vitro susceptibility of recent isolates of Schistosoma mansoni to PZQ in comparison with well-established strains in the laboratory. Material and methods: The in vitro activity of PZQ (6.5-0.003 µg/mL) was evaluated in terms of mortality, reduced motor activity and ultrastructural alterations against S. mansoni. Results: After 3 h of incubation, PZQ, at 6.5 µg/mL, caused 100% mortality of all adult worms in the three types of recent isolates, while PZQ was inactive at concentrations of 0.08-0.003 µg/mL after 3 h of incubation. The results show that the SLM and Sotave isolates basically presented the same pattern of susceptibility, differing only in the concentration of 6.5 µg/mL, where deaths occurred from the range of 1.5 h in Sotave and just in the 3 h range of SLM. Additionally, this article presents ultrastructural evidence of rapid severe PZQ-induced surface membrane damage in S. mansoni after treatment with the drug, such as disintegration, sloughing, and erosion of the surface. Conclusion: According to these results, PZQ is very effective to induce tegument destruction of recent isolates of S. mansoni. |
format |
Article in Journal/Newspaper |
author |
Adriana Maria B. MENDONÇA Ana Paula S. FEITOSA Dyana L. VERAS Thiago J. MATOS-ROCHA Marília G. dos Santos CAVALCANTI Constança Clara G. S. BARBOSA Fábio A. BRAYNER Luiz C. ALVES |
author_facet |
Adriana Maria B. MENDONÇA Ana Paula S. FEITOSA Dyana L. VERAS Thiago J. MATOS-ROCHA Marília G. dos Santos CAVALCANTI Constança Clara G. S. BARBOSA Fábio A. BRAYNER Luiz C. ALVES |
author_sort |
Adriana Maria B. MENDONÇA |
title |
THE SUSCEPTIBILITY OF RECENT ISOLATES OF Schistosoma mansoni TO PRAZIQUANTEL |
title_short |
THE SUSCEPTIBILITY OF RECENT ISOLATES OF Schistosoma mansoni TO PRAZIQUANTEL |
title_full |
THE SUSCEPTIBILITY OF RECENT ISOLATES OF Schistosoma mansoni TO PRAZIQUANTEL |
title_fullStr |
THE SUSCEPTIBILITY OF RECENT ISOLATES OF Schistosoma mansoni TO PRAZIQUANTEL |
title_full_unstemmed |
THE SUSCEPTIBILITY OF RECENT ISOLATES OF Schistosoma mansoni TO PRAZIQUANTEL |
title_sort |
susceptibility of recent isolates of schistosoma mansoni to praziquantel |
publisher |
Universidade de São Paulo (USP) |
publishDate |
2016 |
url |
https://doi.org/10.1590/S1678-9946201658007 https://doaj.org/article/ed372cac38ab4fac9117e2b98f351d0c |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 58, Iss 0 (2016) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652016005000206&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/S1678-9946201658007 https://doaj.org/article/ed372cac38ab4fac9117e2b98f351d0c |
op_doi |
https://doi.org/10.1590/S1678-9946201658007 |
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Revista do Instituto de Medicina Tropical de São Paulo |
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58 |
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