wrmXpress: A modular package for high-throughput image analysis of parasitic and free-living worms.
Advances in high-throughput and high-content imaging technologies require concomitant development of analytical software capable of handling large datasets and generating relevant phenotypic measurements. Several tools have been developed to analyze drug response phenotypes in parasitic and free-liv...
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Online Access: | https://doi.org/10.1371/journal.pntd.0010937 https://doaj.org/article/ecf78f1d8cdc428ab8c3a1f69be0d62c |
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ftdoajarticles:oai:doaj.org/article:ecf78f1d8cdc428ab8c3a1f69be0d62c 2023-05-15T15:05:41+02:00 wrmXpress: A modular package for high-throughput image analysis of parasitic and free-living worms. Nicolas J Wheeler Kendra J Gallo Elena J G Rehborg Kaetlyn T Ryan John D Chan Mostafa Zamanian 2022-11-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0010937 https://doaj.org/article/ecf78f1d8cdc428ab8c3a1f69be0d62c EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0010937 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0010937 https://doaj.org/article/ecf78f1d8cdc428ab8c3a1f69be0d62c PLoS Neglected Tropical Diseases, Vol 16, Iss 11, p e0010937 (2022) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2022 ftdoajarticles https://doi.org/10.1371/journal.pntd.0010937 2023-03-05T01:27:41Z Advances in high-throughput and high-content imaging technologies require concomitant development of analytical software capable of handling large datasets and generating relevant phenotypic measurements. Several tools have been developed to analyze drug response phenotypes in parasitic and free-living worms, but these are siloed and often limited to specific instrumentation, worm species, and single phenotypes. No unified tool exists to analyze diverse high-content phenotypic imaging data of worms and provide a platform for future extensibility. We have developed wrmXpress, a unified framework for analyzing a variety of phenotypes matched to high-content experimental assays of free-living and parasitic nematodes and flatworms. We demonstrate its utility for analyzing a suite of phenotypes, including motility, development/size, fecundity, and feeding, and establish the package as a platform upon which to build future custom phenotypic modules. We show that wrmXpress can serve as an analytical workhorse for anthelmintic screening efforts across schistosomes, filarial nematodes, and free-living model nematodes and holds promise for enabling collaboration among investigators with diverse interests. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 16 11 e0010937 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Nicolas J Wheeler Kendra J Gallo Elena J G Rehborg Kaetlyn T Ryan John D Chan Mostafa Zamanian wrmXpress: A modular package for high-throughput image analysis of parasitic and free-living worms. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Advances in high-throughput and high-content imaging technologies require concomitant development of analytical software capable of handling large datasets and generating relevant phenotypic measurements. Several tools have been developed to analyze drug response phenotypes in parasitic and free-living worms, but these are siloed and often limited to specific instrumentation, worm species, and single phenotypes. No unified tool exists to analyze diverse high-content phenotypic imaging data of worms and provide a platform for future extensibility. We have developed wrmXpress, a unified framework for analyzing a variety of phenotypes matched to high-content experimental assays of free-living and parasitic nematodes and flatworms. We demonstrate its utility for analyzing a suite of phenotypes, including motility, development/size, fecundity, and feeding, and establish the package as a platform upon which to build future custom phenotypic modules. We show that wrmXpress can serve as an analytical workhorse for anthelmintic screening efforts across schistosomes, filarial nematodes, and free-living model nematodes and holds promise for enabling collaboration among investigators with diverse interests. |
format |
Article in Journal/Newspaper |
author |
Nicolas J Wheeler Kendra J Gallo Elena J G Rehborg Kaetlyn T Ryan John D Chan Mostafa Zamanian |
author_facet |
Nicolas J Wheeler Kendra J Gallo Elena J G Rehborg Kaetlyn T Ryan John D Chan Mostafa Zamanian |
author_sort |
Nicolas J Wheeler |
title |
wrmXpress: A modular package for high-throughput image analysis of parasitic and free-living worms. |
title_short |
wrmXpress: A modular package for high-throughput image analysis of parasitic and free-living worms. |
title_full |
wrmXpress: A modular package for high-throughput image analysis of parasitic and free-living worms. |
title_fullStr |
wrmXpress: A modular package for high-throughput image analysis of parasitic and free-living worms. |
title_full_unstemmed |
wrmXpress: A modular package for high-throughput image analysis of parasitic and free-living worms. |
title_sort |
wrmxpress: a modular package for high-throughput image analysis of parasitic and free-living worms. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2022 |
url |
https://doi.org/10.1371/journal.pntd.0010937 https://doaj.org/article/ecf78f1d8cdc428ab8c3a1f69be0d62c |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 16, Iss 11, p e0010937 (2022) |
op_relation |
https://doi.org/10.1371/journal.pntd.0010937 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0010937 https://doaj.org/article/ecf78f1d8cdc428ab8c3a1f69be0d62c |
op_doi |
https://doi.org/10.1371/journal.pntd.0010937 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
16 |
container_issue |
11 |
container_start_page |
e0010937 |
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1766337331702267904 |