Piperine suppresses growth and migration of human breast cancer cells through attenuation of Rac1 expression
Objective: To investigate the effect of piperine on human breast cancer cells. Methods: The effect of piperine on proliferation and migration of human breast cancer cells, MCF-7 and MDA-MB-231, was investigated using colony formation assays, wound healing assays, Matrigel migration assays, flow cyto...
Published in: | Asian Pacific Journal of Tropical Biomedicine |
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Wolters Kluwer Medknow Publications
2022
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Online Access: | https://doi.org/10.4103/2221-1691.333211 https://doaj.org/article/ece2d3826bf94c8ba58f20341ea0aa9d |
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ftdoajarticles:oai:doaj.org/article:ece2d3826bf94c8ba58f20341ea0aa9d 2023-05-15T15:05:13+02:00 Piperine suppresses growth and migration of human breast cancer cells through attenuation of Rac1 expression Benjaporn Buranrat Mutita Junking 2022-01-01T00:00:00Z https://doi.org/10.4103/2221-1691.333211 https://doaj.org/article/ece2d3826bf94c8ba58f20341ea0aa9d EN eng Wolters Kluwer Medknow Publications http://www.apjtb.org/article.asp?issn=2221-1691;year=2022;volume=12;issue=1;spage=39;epage=46;aulast=Buranrat https://doaj.org/toc/2221-1691 https://doaj.org/toc/2588-9222 2221-1691 2588-9222 doi:10.4103/2221-1691.333211 https://doaj.org/article/ece2d3826bf94c8ba58f20341ea0aa9d Asian Pacific Journal of Tropical Biomedicine, Vol 12, Iss 1, Pp 39-46 (2022) piperine breast cancer cells rac1 cell cycle cell migration mcf-7 mda-mb-231 Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2022 ftdoajarticles https://doi.org/10.4103/2221-1691.333211 2022-12-31T11:07:51Z Objective: To investigate the effect of piperine on human breast cancer cells. Methods: The effect of piperine on proliferation and migration of human breast cancer cells, MCF-7 and MDA-MB-231, was investigated using colony formation assays, wound healing assays, Matrigel migration assays, flow cytometry, RT-qPCR, and Western blotting assays. Results: Piperine inhibited the growth of MCF-7 and MDA-MB-231 cells and suppressed colony formation. Cell reduction at the G0/ G1 phase and cell arrest at the G2/M phase were observed in breast cancer cells. However, the significant effect was only demonstrated in MDA-MB-231 cells. Moreover, cancer cell migration was suppressed by piperine at low concentration. RT-qPCR and Western blotting assays showed that piperine downregulated Rac1 gene and protein expression. Conclusions: Piperine could inhibit growth and migration of breast cancer cells by reducing Rac1 gene and protein expression. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 12 1 39 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
piperine breast cancer cells rac1 cell cycle cell migration mcf-7 mda-mb-231 Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
piperine breast cancer cells rac1 cell cycle cell migration mcf-7 mda-mb-231 Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Benjaporn Buranrat Mutita Junking Piperine suppresses growth and migration of human breast cancer cells through attenuation of Rac1 expression |
topic_facet |
piperine breast cancer cells rac1 cell cycle cell migration mcf-7 mda-mb-231 Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To investigate the effect of piperine on human breast cancer cells. Methods: The effect of piperine on proliferation and migration of human breast cancer cells, MCF-7 and MDA-MB-231, was investigated using colony formation assays, wound healing assays, Matrigel migration assays, flow cytometry, RT-qPCR, and Western blotting assays. Results: Piperine inhibited the growth of MCF-7 and MDA-MB-231 cells and suppressed colony formation. Cell reduction at the G0/ G1 phase and cell arrest at the G2/M phase were observed in breast cancer cells. However, the significant effect was only demonstrated in MDA-MB-231 cells. Moreover, cancer cell migration was suppressed by piperine at low concentration. RT-qPCR and Western blotting assays showed that piperine downregulated Rac1 gene and protein expression. Conclusions: Piperine could inhibit growth and migration of breast cancer cells by reducing Rac1 gene and protein expression. |
format |
Article in Journal/Newspaper |
author |
Benjaporn Buranrat Mutita Junking |
author_facet |
Benjaporn Buranrat Mutita Junking |
author_sort |
Benjaporn Buranrat |
title |
Piperine suppresses growth and migration of human breast cancer cells through attenuation of Rac1 expression |
title_short |
Piperine suppresses growth and migration of human breast cancer cells through attenuation of Rac1 expression |
title_full |
Piperine suppresses growth and migration of human breast cancer cells through attenuation of Rac1 expression |
title_fullStr |
Piperine suppresses growth and migration of human breast cancer cells through attenuation of Rac1 expression |
title_full_unstemmed |
Piperine suppresses growth and migration of human breast cancer cells through attenuation of Rac1 expression |
title_sort |
piperine suppresses growth and migration of human breast cancer cells through attenuation of rac1 expression |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2022 |
url |
https://doi.org/10.4103/2221-1691.333211 https://doaj.org/article/ece2d3826bf94c8ba58f20341ea0aa9d |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 12, Iss 1, Pp 39-46 (2022) |
op_relation |
http://www.apjtb.org/article.asp?issn=2221-1691;year=2022;volume=12;issue=1;spage=39;epage=46;aulast=Buranrat https://doaj.org/toc/2221-1691 https://doaj.org/toc/2588-9222 2221-1691 2588-9222 doi:10.4103/2221-1691.333211 https://doaj.org/article/ece2d3826bf94c8ba58f20341ea0aa9d |
op_doi |
https://doi.org/10.4103/2221-1691.333211 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
12 |
container_issue |
1 |
container_start_page |
39 |
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1766336959469322240 |