A novel high performing multiplex immunoassay Multi-HTLV for serological confirmation and typing of HTLV infections.
Background Human T-Cell Lymphotropic Viruses (HTLV) type 1 and type 2 account for an estimated 5 to 10 million infections worldwide and are transmitted through breast feeding, sexual contacts and contaminated cellular blood components. HTLV-associated syndromes are considered as neglected diseases f...
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ftdoajarticles:oai:doaj.org/article:ecd13555300a418899d39605bdd8a8cf 2023-05-15T15:15:51+02:00 A novel high performing multiplex immunoassay Multi-HTLV for serological confirmation and typing of HTLV infections. Lola Marqué Peter Liehl Jasper De Boer Hans Pottel Edward L Murphy Roberta Bruhn Mars Stone Zhanna Kaidarova Tzong-Hae Lee Michael Busch Maan Zrein 2021-11-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0009925 https://doaj.org/article/ecd13555300a418899d39605bdd8a8cf EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0009925 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009925 https://doaj.org/article/ecd13555300a418899d39605bdd8a8cf PLoS Neglected Tropical Diseases, Vol 15, Iss 11, p e0009925 (2021) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2021 ftdoajarticles https://doi.org/10.1371/journal.pntd.0009925 2022-12-31T15:14:00Z Background Human T-Cell Lymphotropic Viruses (HTLV) type 1 and type 2 account for an estimated 5 to 10 million infections worldwide and are transmitted through breast feeding, sexual contacts and contaminated cellular blood components. HTLV-associated syndromes are considered as neglected diseases for which there are no vaccines or therapies available, making it particularly important to ensure the best possible diagnosis to enable proper counselling of infected persons and avoid secondary transmission. Although high quality antibody screening assays are available, currently available confirmatory tests are costly and have variable performance, with high rates of indeterminate and non-typable results reported in many regions of the world. The objective of this project was to develop and validate a new high-performance multiplex immunoassay for confirmation and discrimination of HTLV-1 and HTLV-2 strains. Methodology/principal findings The multiplex platform was used first as a tool to identify suitable antigens and in a second step for assay development. With data generated on over 400 HTLV-positive blood donors sourced from USA and French blood banks, we developed and validated a high-precision interpretation algorithm. The Multi-HTLV assay demonstrated very high performance for confirmation and strain discrimination with 100% sensitivity, 98.1% specificity and 100% of typing accuracy in validation samples. The assay can be interpreted either visually or automatically with a colorimetric image reader and custom algorithm, providing highly reliable results. Conclusions/significance The newly developed Multi-HTLV is very competitive with currently used confirmatory assays and reduces considerably the number of indeterminate results. The multiparametric nature of the assay opens new avenues to study specific serological signatures of each patient, follow the evolution of infection, and explore utility for HTLV disease prognosis. Improving HTLV diagnostic testing will be critical to reduce transmission and to ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 15 11 e0009925 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Lola Marqué Peter Liehl Jasper De Boer Hans Pottel Edward L Murphy Roberta Bruhn Mars Stone Zhanna Kaidarova Tzong-Hae Lee Michael Busch Maan Zrein A novel high performing multiplex immunoassay Multi-HTLV for serological confirmation and typing of HTLV infections. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Background Human T-Cell Lymphotropic Viruses (HTLV) type 1 and type 2 account for an estimated 5 to 10 million infections worldwide and are transmitted through breast feeding, sexual contacts and contaminated cellular blood components. HTLV-associated syndromes are considered as neglected diseases for which there are no vaccines or therapies available, making it particularly important to ensure the best possible diagnosis to enable proper counselling of infected persons and avoid secondary transmission. Although high quality antibody screening assays are available, currently available confirmatory tests are costly and have variable performance, with high rates of indeterminate and non-typable results reported in many regions of the world. The objective of this project was to develop and validate a new high-performance multiplex immunoassay for confirmation and discrimination of HTLV-1 and HTLV-2 strains. Methodology/principal findings The multiplex platform was used first as a tool to identify suitable antigens and in a second step for assay development. With data generated on over 400 HTLV-positive blood donors sourced from USA and French blood banks, we developed and validated a high-precision interpretation algorithm. The Multi-HTLV assay demonstrated very high performance for confirmation and strain discrimination with 100% sensitivity, 98.1% specificity and 100% of typing accuracy in validation samples. The assay can be interpreted either visually or automatically with a colorimetric image reader and custom algorithm, providing highly reliable results. Conclusions/significance The newly developed Multi-HTLV is very competitive with currently used confirmatory assays and reduces considerably the number of indeterminate results. The multiparametric nature of the assay opens new avenues to study specific serological signatures of each patient, follow the evolution of infection, and explore utility for HTLV disease prognosis. Improving HTLV diagnostic testing will be critical to reduce transmission and to ... |
format |
Article in Journal/Newspaper |
author |
Lola Marqué Peter Liehl Jasper De Boer Hans Pottel Edward L Murphy Roberta Bruhn Mars Stone Zhanna Kaidarova Tzong-Hae Lee Michael Busch Maan Zrein |
author_facet |
Lola Marqué Peter Liehl Jasper De Boer Hans Pottel Edward L Murphy Roberta Bruhn Mars Stone Zhanna Kaidarova Tzong-Hae Lee Michael Busch Maan Zrein |
author_sort |
Lola Marqué |
title |
A novel high performing multiplex immunoassay Multi-HTLV for serological confirmation and typing of HTLV infections. |
title_short |
A novel high performing multiplex immunoassay Multi-HTLV for serological confirmation and typing of HTLV infections. |
title_full |
A novel high performing multiplex immunoassay Multi-HTLV for serological confirmation and typing of HTLV infections. |
title_fullStr |
A novel high performing multiplex immunoassay Multi-HTLV for serological confirmation and typing of HTLV infections. |
title_full_unstemmed |
A novel high performing multiplex immunoassay Multi-HTLV for serological confirmation and typing of HTLV infections. |
title_sort |
novel high performing multiplex immunoassay multi-htlv for serological confirmation and typing of htlv infections. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2021 |
url |
https://doi.org/10.1371/journal.pntd.0009925 https://doaj.org/article/ecd13555300a418899d39605bdd8a8cf |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 15, Iss 11, p e0009925 (2021) |
op_relation |
https://doi.org/10.1371/journal.pntd.0009925 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009925 https://doaj.org/article/ecd13555300a418899d39605bdd8a8cf |
op_doi |
https://doi.org/10.1371/journal.pntd.0009925 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
15 |
container_issue |
11 |
container_start_page |
e0009925 |
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1766346193395253248 |