Antagonistic and plant growth promoting properties of actinomycetes from rhizosphere Deschampsia antarctica È. Desv. (Galindez Island, Antarctica)
Aim. The isolation of actinomycetes from Deschampsia antarctica É. Desv. (Galindez Island, Antarctica) rhisosphere and determine their ability to produce compounds with antimicrobial and plant growth promotional properties. Methods. Actinomycetes were isolated using the three different methods: dire...
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State Institution National Antarctic Scientific Center
2019
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Online Access: | https://doi.org/10.33275/1727-7485.1(18).2019.140 https://doaj.org/article/ecc8b3465ad04e25a2c7416da7c3bddd |
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ftdoajarticles:oai:doaj.org/article:ecc8b3465ad04e25a2c7416da7c3bddd 2023-05-15T13:54:03+02:00 Antagonistic and plant growth promoting properties of actinomycetes from rhizosphere Deschampsia antarctica È. Desv. (Galindez Island, Antarctica) S. Tistechok M. Skvortsova A. Luzhetskyy V. Fedorenko I. Parnikoza O. Gromyko 2019-12-01T00:00:00Z https://doi.org/10.33275/1727-7485.1(18).2019.140 https://doaj.org/article/ecc8b3465ad04e25a2c7416da7c3bddd EN UK eng ukr State Institution National Antarctic Scientific Center http://uaj.uac.gov.ua/index.php/uaj/article/view/140 https://doaj.org/toc/1727-7485 https://doaj.org/toc/2415-3087 1727-7485 2415-3087 doi:10.33275/1727-7485.1(18).2019.140 https://doaj.org/article/ecc8b3465ad04e25a2c7416da7c3bddd Український антарктичний журнал, Iss 1(18), Pp 169-177 (2019) antarctic actinomycetes rhizospheric microorganisms antimicrobial activity plant growth promotional properties Meteorology. Climatology QC851-999 Geophysics. Cosmic physics QC801-809 article 2019 ftdoajarticles https://doi.org/10.33275/1727-7485.1(18).2019.140 2022-12-31T13:39:01Z Aim. The isolation of actinomycetes from Deschampsia antarctica É. Desv. (Galindez Island, Antarctica) rhisosphere and determine their ability to produce compounds with antimicrobial and plant growth promotional properties. Methods. Actinomycetes were isolated using the three different methods: direct inoculation, the roots treatment with an aqueous solution of phenol and heated at 100 °C for 60 minutes. To study the antibacterial activity of actinomycetes, they were plated on the Oatmeal medium and flooded by 0,7% agar with specific test-culture. Antifungal activities were studied by putting the agar block with fungal culture on Petri plates with cultures of actinomycetes. An activity index was determined by the ratio of the diameter zone of the inhibit growth test-cultures to the diameter of the actinomycetes colonies. Plant growth promotion properties were studied by commonly accepted methods. Results. 35 psychotolerant isolates were identified among the 43 actinomycetes isolated from D. antarctica rhizosphere. Almost 42% of actinomycetes isolates were antagonists at least one of typical strain of phytopathogenic bacteria (Pseudomonas savastanoi pv. phaseolicola, Xanthomonas campestris pv. campestris, Agrobacterium tumifaciens, Erwinia amylovora) or fungi (Fusarium oxysporum, Botrytis cinerea, Alternaria alternata). An activity index of most isolates was 3—6, in some isolates — 30—32. The potential plant growth promotion properties of isolates were evaluated. 11 isolates of actinomycetes produced indolyl-3-acetic acid (the level of synthesis was 21,0—62,5 μg/ml), 27 isolates produced siderophores and 6 isolates solubilized phosphorus compounds. Conclusions. Antimicrobial and plant growth promotional properties of the actinomycetes from rhizosphere D. antarctica were evaluated. Phytopathogenic bacteria and fungi antagonists were identified. A number of isolates were characterized by plant growth promotional properties that are combined with the synthesis of antimicrobial compounds. Such properties of isolated ... Article in Journal/Newspaper Antarc* Antarctic Antarctica Galindez Island Directory of Open Access Journals: DOAJ Articles Antarctic Galindez ENVELOPE(-64.248,-64.248,-65.250,-65.250) Galindez Island ENVELOPE(-64.267,-64.267,-65.250,-65.250) Ukrainian Antarctic Journal 1(18) 169 177 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English Ukrainian |
topic |
antarctic actinomycetes rhizospheric microorganisms antimicrobial activity plant growth promotional properties Meteorology. Climatology QC851-999 Geophysics. Cosmic physics QC801-809 |
spellingShingle |
antarctic actinomycetes rhizospheric microorganisms antimicrobial activity plant growth promotional properties Meteorology. Climatology QC851-999 Geophysics. Cosmic physics QC801-809 S. Tistechok M. Skvortsova A. Luzhetskyy V. Fedorenko I. Parnikoza O. Gromyko Antagonistic and plant growth promoting properties of actinomycetes from rhizosphere Deschampsia antarctica È. Desv. (Galindez Island, Antarctica) |
topic_facet |
antarctic actinomycetes rhizospheric microorganisms antimicrobial activity plant growth promotional properties Meteorology. Climatology QC851-999 Geophysics. Cosmic physics QC801-809 |
description |
Aim. The isolation of actinomycetes from Deschampsia antarctica É. Desv. (Galindez Island, Antarctica) rhisosphere and determine their ability to produce compounds with antimicrobial and plant growth promotional properties. Methods. Actinomycetes were isolated using the three different methods: direct inoculation, the roots treatment with an aqueous solution of phenol and heated at 100 °C for 60 minutes. To study the antibacterial activity of actinomycetes, they were plated on the Oatmeal medium and flooded by 0,7% agar with specific test-culture. Antifungal activities were studied by putting the agar block with fungal culture on Petri plates with cultures of actinomycetes. An activity index was determined by the ratio of the diameter zone of the inhibit growth test-cultures to the diameter of the actinomycetes colonies. Plant growth promotion properties were studied by commonly accepted methods. Results. 35 psychotolerant isolates were identified among the 43 actinomycetes isolated from D. antarctica rhizosphere. Almost 42% of actinomycetes isolates were antagonists at least one of typical strain of phytopathogenic bacteria (Pseudomonas savastanoi pv. phaseolicola, Xanthomonas campestris pv. campestris, Agrobacterium tumifaciens, Erwinia amylovora) or fungi (Fusarium oxysporum, Botrytis cinerea, Alternaria alternata). An activity index of most isolates was 3—6, in some isolates — 30—32. The potential plant growth promotion properties of isolates were evaluated. 11 isolates of actinomycetes produced indolyl-3-acetic acid (the level of synthesis was 21,0—62,5 μg/ml), 27 isolates produced siderophores and 6 isolates solubilized phosphorus compounds. Conclusions. Antimicrobial and plant growth promotional properties of the actinomycetes from rhizosphere D. antarctica were evaluated. Phytopathogenic bacteria and fungi antagonists were identified. A number of isolates were characterized by plant growth promotional properties that are combined with the synthesis of antimicrobial compounds. Such properties of isolated ... |
format |
Article in Journal/Newspaper |
author |
S. Tistechok M. Skvortsova A. Luzhetskyy V. Fedorenko I. Parnikoza O. Gromyko |
author_facet |
S. Tistechok M. Skvortsova A. Luzhetskyy V. Fedorenko I. Parnikoza O. Gromyko |
author_sort |
S. Tistechok |
title |
Antagonistic and plant growth promoting properties of actinomycetes from rhizosphere Deschampsia antarctica È. Desv. (Galindez Island, Antarctica) |
title_short |
Antagonistic and plant growth promoting properties of actinomycetes from rhizosphere Deschampsia antarctica È. Desv. (Galindez Island, Antarctica) |
title_full |
Antagonistic and plant growth promoting properties of actinomycetes from rhizosphere Deschampsia antarctica È. Desv. (Galindez Island, Antarctica) |
title_fullStr |
Antagonistic and plant growth promoting properties of actinomycetes from rhizosphere Deschampsia antarctica È. Desv. (Galindez Island, Antarctica) |
title_full_unstemmed |
Antagonistic and plant growth promoting properties of actinomycetes from rhizosphere Deschampsia antarctica È. Desv. (Galindez Island, Antarctica) |
title_sort |
antagonistic and plant growth promoting properties of actinomycetes from rhizosphere deschampsia antarctica è. desv. (galindez island, antarctica) |
publisher |
State Institution National Antarctic Scientific Center |
publishDate |
2019 |
url |
https://doi.org/10.33275/1727-7485.1(18).2019.140 https://doaj.org/article/ecc8b3465ad04e25a2c7416da7c3bddd |
long_lat |
ENVELOPE(-64.248,-64.248,-65.250,-65.250) ENVELOPE(-64.267,-64.267,-65.250,-65.250) |
geographic |
Antarctic Galindez Galindez Island |
geographic_facet |
Antarctic Galindez Galindez Island |
genre |
Antarc* Antarctic Antarctica Galindez Island |
genre_facet |
Antarc* Antarctic Antarctica Galindez Island |
op_source |
Український антарктичний журнал, Iss 1(18), Pp 169-177 (2019) |
op_relation |
http://uaj.uac.gov.ua/index.php/uaj/article/view/140 https://doaj.org/toc/1727-7485 https://doaj.org/toc/2415-3087 1727-7485 2415-3087 doi:10.33275/1727-7485.1(18).2019.140 https://doaj.org/article/ecc8b3465ad04e25a2c7416da7c3bddd |
op_doi |
https://doi.org/10.33275/1727-7485.1(18).2019.140 |
container_title |
Ukrainian Antarctic Journal |
container_issue |
1(18) |
container_start_page |
169 |
op_container_end_page |
177 |
_version_ |
1766259564514115584 |