Mass blood survey for malaria: pooling and real-time PCR combined with expert microscopy in north-west Thailand

Abstract Background Asymptomatic carriage of Plasmodium falciparum and Plasmodium vivax is common in both low-and high-transmission settings and represents an important reservoir of infection that needs to be targeted if malaria elimination is to succeed. Methods Mass blood examinations (475 individ...

Full description

Bibliographic Details
Published in:Malaria Journal
Main Authors: Congpuong Kanungnit, SaeJeng Aungkana, Sug-aram Rungniran, Aruncharus Supannee, Darakapong Ampai, Meshnick Steven R, Satimai Wichai
Format: Article in Journal/Newspaper
Language:English
Published: BMC 2012
Subjects:
Online Access:https://doi.org/10.1186/1475-2875-11-288
https://doaj.org/article/ecc7fdb4f96349c29ecde8c82e66b26e
id ftdoajarticles:oai:doaj.org/article:ecc7fdb4f96349c29ecde8c82e66b26e
record_format openpolar
spelling ftdoajarticles:oai:doaj.org/article:ecc7fdb4f96349c29ecde8c82e66b26e 2023-05-15T15:07:11+02:00 Mass blood survey for malaria: pooling and real-time PCR combined with expert microscopy in north-west Thailand Congpuong Kanungnit SaeJeng Aungkana Sug-aram Rungniran Aruncharus Supannee Darakapong Ampai Meshnick Steven R Satimai Wichai 2012-08-01T00:00:00Z https://doi.org/10.1186/1475-2875-11-288 https://doaj.org/article/ecc7fdb4f96349c29ecde8c82e66b26e EN eng BMC http://www.malariajournal.com/content/11/1/288 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-11-288 1475-2875 https://doaj.org/article/ecc7fdb4f96349c29ecde8c82e66b26e Malaria Journal, Vol 11, Iss 1, p 288 (2012) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2012 ftdoajarticles https://doi.org/10.1186/1475-2875-11-288 2022-12-31T07:13:27Z Abstract Background Asymptomatic carriage of Plasmodium falciparum and Plasmodium vivax is common in both low-and high-transmission settings and represents an important reservoir of infection that needs to be targeted if malaria elimination is to succeed. Methods Mass blood examinations (475 individuals) were conducted in two villages in Mae Hong Son, an area of endemic but low-transmission malaria in the north-west of Thailand. The microscopist at the local malaria clinic did not detect any infections. Pools of four samples were screened by real-time PCR; individual members of all of the positive pools were then re-examined by expert microscopy and by a second species-specific PCR reaction. Results Eight subjects were found to be positive by both PCR and expert microscopy and one was found to be positive by PCR alone. The slides contained asexual stage parasites of P. vivax, P. falciparum and Plasmodium malariae, but no gametocytes. The local clinic was notified within two to eight days of the survey. Conclusion A combination of pooling, real-time PCR and expert microscopy provides a feasible approach to identifying and treating asymptomatic malaria infections in a timely manner. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 11 1
institution Open Polar
collection Directory of Open Access Journals: DOAJ Articles
op_collection_id ftdoajarticles
language English
topic Arctic medicine. Tropical medicine
RC955-962
Infectious and parasitic diseases
RC109-216
spellingShingle Arctic medicine. Tropical medicine
RC955-962
Infectious and parasitic diseases
RC109-216
Congpuong Kanungnit
SaeJeng Aungkana
Sug-aram Rungniran
Aruncharus Supannee
Darakapong Ampai
Meshnick Steven R
Satimai Wichai
Mass blood survey for malaria: pooling and real-time PCR combined with expert microscopy in north-west Thailand
topic_facet Arctic medicine. Tropical medicine
RC955-962
Infectious and parasitic diseases
RC109-216
description Abstract Background Asymptomatic carriage of Plasmodium falciparum and Plasmodium vivax is common in both low-and high-transmission settings and represents an important reservoir of infection that needs to be targeted if malaria elimination is to succeed. Methods Mass blood examinations (475 individuals) were conducted in two villages in Mae Hong Son, an area of endemic but low-transmission malaria in the north-west of Thailand. The microscopist at the local malaria clinic did not detect any infections. Pools of four samples were screened by real-time PCR; individual members of all of the positive pools were then re-examined by expert microscopy and by a second species-specific PCR reaction. Results Eight subjects were found to be positive by both PCR and expert microscopy and one was found to be positive by PCR alone. The slides contained asexual stage parasites of P. vivax, P. falciparum and Plasmodium malariae, but no gametocytes. The local clinic was notified within two to eight days of the survey. Conclusion A combination of pooling, real-time PCR and expert microscopy provides a feasible approach to identifying and treating asymptomatic malaria infections in a timely manner.
format Article in Journal/Newspaper
author Congpuong Kanungnit
SaeJeng Aungkana
Sug-aram Rungniran
Aruncharus Supannee
Darakapong Ampai
Meshnick Steven R
Satimai Wichai
author_facet Congpuong Kanungnit
SaeJeng Aungkana
Sug-aram Rungniran
Aruncharus Supannee
Darakapong Ampai
Meshnick Steven R
Satimai Wichai
author_sort Congpuong Kanungnit
title Mass blood survey for malaria: pooling and real-time PCR combined with expert microscopy in north-west Thailand
title_short Mass blood survey for malaria: pooling and real-time PCR combined with expert microscopy in north-west Thailand
title_full Mass blood survey for malaria: pooling and real-time PCR combined with expert microscopy in north-west Thailand
title_fullStr Mass blood survey for malaria: pooling and real-time PCR combined with expert microscopy in north-west Thailand
title_full_unstemmed Mass blood survey for malaria: pooling and real-time PCR combined with expert microscopy in north-west Thailand
title_sort mass blood survey for malaria: pooling and real-time pcr combined with expert microscopy in north-west thailand
publisher BMC
publishDate 2012
url https://doi.org/10.1186/1475-2875-11-288
https://doaj.org/article/ecc7fdb4f96349c29ecde8c82e66b26e
geographic Arctic
geographic_facet Arctic
genre Arctic
genre_facet Arctic
op_source Malaria Journal, Vol 11, Iss 1, p 288 (2012)
op_relation http://www.malariajournal.com/content/11/1/288
https://doaj.org/toc/1475-2875
doi:10.1186/1475-2875-11-288
1475-2875
https://doaj.org/article/ecc7fdb4f96349c29ecde8c82e66b26e
op_doi https://doi.org/10.1186/1475-2875-11-288
container_title Malaria Journal
container_volume 11
container_issue 1
_version_ 1766338736989143040