Mass blood survey for malaria: pooling and real-time PCR combined with expert microscopy in north-west Thailand
Abstract Background Asymptomatic carriage of Plasmodium falciparum and Plasmodium vivax is common in both low-and high-transmission settings and represents an important reservoir of infection that needs to be targeted if malaria elimination is to succeed. Methods Mass blood examinations (475 individ...
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ftdoajarticles:oai:doaj.org/article:ecc7fdb4f96349c29ecde8c82e66b26e 2023-05-15T15:07:11+02:00 Mass blood survey for malaria: pooling and real-time PCR combined with expert microscopy in north-west Thailand Congpuong Kanungnit SaeJeng Aungkana Sug-aram Rungniran Aruncharus Supannee Darakapong Ampai Meshnick Steven R Satimai Wichai 2012-08-01T00:00:00Z https://doi.org/10.1186/1475-2875-11-288 https://doaj.org/article/ecc7fdb4f96349c29ecde8c82e66b26e EN eng BMC http://www.malariajournal.com/content/11/1/288 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-11-288 1475-2875 https://doaj.org/article/ecc7fdb4f96349c29ecde8c82e66b26e Malaria Journal, Vol 11, Iss 1, p 288 (2012) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2012 ftdoajarticles https://doi.org/10.1186/1475-2875-11-288 2022-12-31T07:13:27Z Abstract Background Asymptomatic carriage of Plasmodium falciparum and Plasmodium vivax is common in both low-and high-transmission settings and represents an important reservoir of infection that needs to be targeted if malaria elimination is to succeed. Methods Mass blood examinations (475 individuals) were conducted in two villages in Mae Hong Son, an area of endemic but low-transmission malaria in the north-west of Thailand. The microscopist at the local malaria clinic did not detect any infections. Pools of four samples were screened by real-time PCR; individual members of all of the positive pools were then re-examined by expert microscopy and by a second species-specific PCR reaction. Results Eight subjects were found to be positive by both PCR and expert microscopy and one was found to be positive by PCR alone. The slides contained asexual stage parasites of P. vivax, P. falciparum and Plasmodium malariae, but no gametocytes. The local clinic was notified within two to eight days of the survey. Conclusion A combination of pooling, real-time PCR and expert microscopy provides a feasible approach to identifying and treating asymptomatic malaria infections in a timely manner. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 11 1 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Congpuong Kanungnit SaeJeng Aungkana Sug-aram Rungniran Aruncharus Supannee Darakapong Ampai Meshnick Steven R Satimai Wichai Mass blood survey for malaria: pooling and real-time PCR combined with expert microscopy in north-west Thailand |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Asymptomatic carriage of Plasmodium falciparum and Plasmodium vivax is common in both low-and high-transmission settings and represents an important reservoir of infection that needs to be targeted if malaria elimination is to succeed. Methods Mass blood examinations (475 individuals) were conducted in two villages in Mae Hong Son, an area of endemic but low-transmission malaria in the north-west of Thailand. The microscopist at the local malaria clinic did not detect any infections. Pools of four samples were screened by real-time PCR; individual members of all of the positive pools were then re-examined by expert microscopy and by a second species-specific PCR reaction. Results Eight subjects were found to be positive by both PCR and expert microscopy and one was found to be positive by PCR alone. The slides contained asexual stage parasites of P. vivax, P. falciparum and Plasmodium malariae, but no gametocytes. The local clinic was notified within two to eight days of the survey. Conclusion A combination of pooling, real-time PCR and expert microscopy provides a feasible approach to identifying and treating asymptomatic malaria infections in a timely manner. |
format |
Article in Journal/Newspaper |
author |
Congpuong Kanungnit SaeJeng Aungkana Sug-aram Rungniran Aruncharus Supannee Darakapong Ampai Meshnick Steven R Satimai Wichai |
author_facet |
Congpuong Kanungnit SaeJeng Aungkana Sug-aram Rungniran Aruncharus Supannee Darakapong Ampai Meshnick Steven R Satimai Wichai |
author_sort |
Congpuong Kanungnit |
title |
Mass blood survey for malaria: pooling and real-time PCR combined with expert microscopy in north-west Thailand |
title_short |
Mass blood survey for malaria: pooling and real-time PCR combined with expert microscopy in north-west Thailand |
title_full |
Mass blood survey for malaria: pooling and real-time PCR combined with expert microscopy in north-west Thailand |
title_fullStr |
Mass blood survey for malaria: pooling and real-time PCR combined with expert microscopy in north-west Thailand |
title_full_unstemmed |
Mass blood survey for malaria: pooling and real-time PCR combined with expert microscopy in north-west Thailand |
title_sort |
mass blood survey for malaria: pooling and real-time pcr combined with expert microscopy in north-west thailand |
publisher |
BMC |
publishDate |
2012 |
url |
https://doi.org/10.1186/1475-2875-11-288 https://doaj.org/article/ecc7fdb4f96349c29ecde8c82e66b26e |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 11, Iss 1, p 288 (2012) |
op_relation |
http://www.malariajournal.com/content/11/1/288 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-11-288 1475-2875 https://doaj.org/article/ecc7fdb4f96349c29ecde8c82e66b26e |
op_doi |
https://doi.org/10.1186/1475-2875-11-288 |
container_title |
Malaria Journal |
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11 |
container_issue |
1 |
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1766338736989143040 |