Development of a low-temperature immersion microscopy technique for ice research
Perennial ice can be studied for many purposes, including paleoclimate records or rheological properties. For most of those purposes, the ice microstructure must be studied, often through optical microscopy. The aim of this work is to assess the viability of immersion microscopy for the study of ice...
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Cambridge University Press
2023
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ftdoajarticles:oai:doaj.org/article:ecaef82faa464cca99315e435c18f828 2024-09-09T19:00:58+00:00 Development of a low-temperature immersion microscopy technique for ice research Bittor Muniozguren-Arostegi Patricia Muñoz-Marzagon Sérgio Henrique Faria 2023-07-01T00:00:00Z https://doi.org/10.1017/aog.2023.74 https://doaj.org/article/ecaef82faa464cca99315e435c18f828 EN eng Cambridge University Press https://www.cambridge.org/core/product/identifier/S0260305523000745/type/journal_article https://doaj.org/toc/0260-3055 https://doaj.org/toc/1727-5644 doi:10.1017/aog.2023.74 0260-3055 1727-5644 https://doaj.org/article/ecaef82faa464cca99315e435c18f828 Annals of Glaciology, Vol 64, Pp 133-139 (2023) ice and climate ice dynamics ice physics Meteorology. Climatology QC851-999 article 2023 ftdoajarticles https://doi.org/10.1017/aog.2023.74 2024-08-05T17:49:28Z Perennial ice can be studied for many purposes, including paleoclimate records or rheological properties. For most of those purposes, the ice microstructure must be studied, often through optical microscopy. The aim of this work is to assess the viability of immersion microscopy for the study of ice microstructures. It consists of using an oil between the objective lens and the specimen, to increase image resolution. Immersion microscopy is a technique well-developed for the investigation of diverse materials, but it has so far not been explored for ice research. Here we investigate the challenges and advantages of that technique. The main challenge is related to the selection of the immersion oil itself, which must satisfy a number of criteria, ranging from refractive index and viscosity to toxicity and reactivity. We identify pure silicone oil (dimethicone) as a simple and safe option for immersion microscopy of inner ice structures. Among its advantages, it provides higher resolution (compared to standard ‘dry’ microscopy) and it can be simultaneously used as a long-term coating to prevent undesired sublimation of the ice-sample surfaces. For the observation of surface structures, however, another type of oil with higher refractive index should be used. Article in Journal/Newspaper Annals of Glaciology Directory of Open Access Journals: DOAJ Articles Annals of Glaciology 1 7 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
ice and climate ice dynamics ice physics Meteorology. Climatology QC851-999 |
spellingShingle |
ice and climate ice dynamics ice physics Meteorology. Climatology QC851-999 Bittor Muniozguren-Arostegi Patricia Muñoz-Marzagon Sérgio Henrique Faria Development of a low-temperature immersion microscopy technique for ice research |
topic_facet |
ice and climate ice dynamics ice physics Meteorology. Climatology QC851-999 |
description |
Perennial ice can be studied for many purposes, including paleoclimate records or rheological properties. For most of those purposes, the ice microstructure must be studied, often through optical microscopy. The aim of this work is to assess the viability of immersion microscopy for the study of ice microstructures. It consists of using an oil between the objective lens and the specimen, to increase image resolution. Immersion microscopy is a technique well-developed for the investigation of diverse materials, but it has so far not been explored for ice research. Here we investigate the challenges and advantages of that technique. The main challenge is related to the selection of the immersion oil itself, which must satisfy a number of criteria, ranging from refractive index and viscosity to toxicity and reactivity. We identify pure silicone oil (dimethicone) as a simple and safe option for immersion microscopy of inner ice structures. Among its advantages, it provides higher resolution (compared to standard ‘dry’ microscopy) and it can be simultaneously used as a long-term coating to prevent undesired sublimation of the ice-sample surfaces. For the observation of surface structures, however, another type of oil with higher refractive index should be used. |
format |
Article in Journal/Newspaper |
author |
Bittor Muniozguren-Arostegi Patricia Muñoz-Marzagon Sérgio Henrique Faria |
author_facet |
Bittor Muniozguren-Arostegi Patricia Muñoz-Marzagon Sérgio Henrique Faria |
author_sort |
Bittor Muniozguren-Arostegi |
title |
Development of a low-temperature immersion microscopy technique for ice research |
title_short |
Development of a low-temperature immersion microscopy technique for ice research |
title_full |
Development of a low-temperature immersion microscopy technique for ice research |
title_fullStr |
Development of a low-temperature immersion microscopy technique for ice research |
title_full_unstemmed |
Development of a low-temperature immersion microscopy technique for ice research |
title_sort |
development of a low-temperature immersion microscopy technique for ice research |
publisher |
Cambridge University Press |
publishDate |
2023 |
url |
https://doi.org/10.1017/aog.2023.74 https://doaj.org/article/ecaef82faa464cca99315e435c18f828 |
genre |
Annals of Glaciology |
genre_facet |
Annals of Glaciology |
op_source |
Annals of Glaciology, Vol 64, Pp 133-139 (2023) |
op_relation |
https://www.cambridge.org/core/product/identifier/S0260305523000745/type/journal_article https://doaj.org/toc/0260-3055 https://doaj.org/toc/1727-5644 doi:10.1017/aog.2023.74 0260-3055 1727-5644 https://doaj.org/article/ecaef82faa464cca99315e435c18f828 |
op_doi |
https://doi.org/10.1017/aog.2023.74 |
container_title |
Annals of Glaciology |
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1 |
op_container_end_page |
7 |
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1809942927709831168 |