Loop-Mediated Isothermal Amplification Test for Trypanosoma gambiense Group 1 with Stem Primers: A Molecular Xenomonitoring Test for Sleeping Sickness
The World Health Organization has targeted Human African Trypanosomiasis (HAT) for elimination by 2020 with zero incidence by 2030. To achieve and sustain this goal, accurate and easy-to-deploy diagnostic tests for Gambian trypanosomiasis which accounts for over 98% of reported cases will play a cru...
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ftdoajarticles:oai:doaj.org/article:ec7af4b9a91e4fda982acbb09f916a99 2024-09-09T19:26:26+00:00 Loop-Mediated Isothermal Amplification Test for Trypanosoma gambiense Group 1 with Stem Primers: A Molecular Xenomonitoring Test for Sleeping Sickness Zablon K. Njiru Cecilia K. Mbae Gitonga N. Mburugu 2017-01-01T00:00:00Z https://doi.org/10.1155/2017/8630708 https://doaj.org/article/ec7af4b9a91e4fda982acbb09f916a99 EN eng Wiley http://dx.doi.org/10.1155/2017/8630708 https://doaj.org/toc/1687-9686 https://doaj.org/toc/1687-9694 1687-9686 1687-9694 doi:10.1155/2017/8630708 https://doaj.org/article/ec7af4b9a91e4fda982acbb09f916a99 Journal of Tropical Medicine, Vol 2017 (2017) Arctic medicine. Tropical medicine RC955-962 article 2017 ftdoajarticles https://doi.org/10.1155/2017/8630708 2024-08-05T17:48:36Z The World Health Organization has targeted Human African Trypanosomiasis (HAT) for elimination by 2020 with zero incidence by 2030. To achieve and sustain this goal, accurate and easy-to-deploy diagnostic tests for Gambian trypanosomiasis which accounts for over 98% of reported cases will play a crucial role. Most needed will be tools for surveillance of pathogen in vectors (xenomonitoring) since population screening tests are readily available. The development of new tests is expensive and takes a long time while incremental improvement of existing technologies that have potential for xenomonitoring may offer a shorter pathway to tools for HAT surveillance. We have investigated the effect of including a second set of reaction accelerating primers (stem primers) to the standard T. brucei gambiense LAMP test format. The new test format was analyzed with and without outer primers. Amplification was carried out using Rotorgene 6000 and the portable ESE Quant amplification unit capable of real-time data output. The stem LAMP formats indicated shorter time to results (~8 min), were 10–100-fold more sensitive, and indicated higher diagnostic sensitivity and accuracy compared to the standard LAMP test. It was possible to confirm the predicted product using ESE melt curves demonstrating the potential of combining LAMP and real-time technologies as possible tool for HAT molecular xenomonitoring. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Tropical Medicine 2017 1 7 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 |
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Arctic medicine. Tropical medicine RC955-962 Zablon K. Njiru Cecilia K. Mbae Gitonga N. Mburugu Loop-Mediated Isothermal Amplification Test for Trypanosoma gambiense Group 1 with Stem Primers: A Molecular Xenomonitoring Test for Sleeping Sickness |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 |
description |
The World Health Organization has targeted Human African Trypanosomiasis (HAT) for elimination by 2020 with zero incidence by 2030. To achieve and sustain this goal, accurate and easy-to-deploy diagnostic tests for Gambian trypanosomiasis which accounts for over 98% of reported cases will play a crucial role. Most needed will be tools for surveillance of pathogen in vectors (xenomonitoring) since population screening tests are readily available. The development of new tests is expensive and takes a long time while incremental improvement of existing technologies that have potential for xenomonitoring may offer a shorter pathway to tools for HAT surveillance. We have investigated the effect of including a second set of reaction accelerating primers (stem primers) to the standard T. brucei gambiense LAMP test format. The new test format was analyzed with and without outer primers. Amplification was carried out using Rotorgene 6000 and the portable ESE Quant amplification unit capable of real-time data output. The stem LAMP formats indicated shorter time to results (~8 min), were 10–100-fold more sensitive, and indicated higher diagnostic sensitivity and accuracy compared to the standard LAMP test. It was possible to confirm the predicted product using ESE melt curves demonstrating the potential of combining LAMP and real-time technologies as possible tool for HAT molecular xenomonitoring. |
format |
Article in Journal/Newspaper |
author |
Zablon K. Njiru Cecilia K. Mbae Gitonga N. Mburugu |
author_facet |
Zablon K. Njiru Cecilia K. Mbae Gitonga N. Mburugu |
author_sort |
Zablon K. Njiru |
title |
Loop-Mediated Isothermal Amplification Test for Trypanosoma gambiense Group 1 with Stem Primers: A Molecular Xenomonitoring Test for Sleeping Sickness |
title_short |
Loop-Mediated Isothermal Amplification Test for Trypanosoma gambiense Group 1 with Stem Primers: A Molecular Xenomonitoring Test for Sleeping Sickness |
title_full |
Loop-Mediated Isothermal Amplification Test for Trypanosoma gambiense Group 1 with Stem Primers: A Molecular Xenomonitoring Test for Sleeping Sickness |
title_fullStr |
Loop-Mediated Isothermal Amplification Test for Trypanosoma gambiense Group 1 with Stem Primers: A Molecular Xenomonitoring Test for Sleeping Sickness |
title_full_unstemmed |
Loop-Mediated Isothermal Amplification Test for Trypanosoma gambiense Group 1 with Stem Primers: A Molecular Xenomonitoring Test for Sleeping Sickness |
title_sort |
loop-mediated isothermal amplification test for trypanosoma gambiense group 1 with stem primers: a molecular xenomonitoring test for sleeping sickness |
publisher |
Wiley |
publishDate |
2017 |
url |
https://doi.org/10.1155/2017/8630708 https://doaj.org/article/ec7af4b9a91e4fda982acbb09f916a99 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Tropical Medicine, Vol 2017 (2017) |
op_relation |
http://dx.doi.org/10.1155/2017/8630708 https://doaj.org/toc/1687-9686 https://doaj.org/toc/1687-9694 1687-9686 1687-9694 doi:10.1155/2017/8630708 https://doaj.org/article/ec7af4b9a91e4fda982acbb09f916a99 |
op_doi |
https://doi.org/10.1155/2017/8630708 |
container_title |
Journal of Tropical Medicine |
container_volume |
2017 |
container_start_page |
1 |
op_container_end_page |
7 |
_version_ |
1809896048126066688 |