In vivo transcriptional profiling of Plasmodium falciparum
Abstract Background Both host and pathogen factors contribute to disease outcome in Plasmodium falciparum infection. The feasibility of studying the P. falciparum in vivo transcriptome to understand parasite transcriptional response while it resides in the human host is presented. Methods A custom m...
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ftdoajarticles:oai:doaj.org/article:ec73661e164a4e48a0efa3dbdd0fd225 2023-05-15T15:06:15+02:00 In vivo transcriptional profiling of Plasmodium falciparum Sultan Ali Mboup Soulyemane Ndir Omar Zhou Yingyao Fang Xuemin Sarr Ousmane Le Roch Karine G Daily Johanna P Winzeler Elizabeth A Wirth Dyann F 2004-08-01T00:00:00Z https://doi.org/10.1186/1475-2875-3-30 https://doaj.org/article/ec73661e164a4e48a0efa3dbdd0fd225 EN eng BMC http://www.malariajournal.com/content/3/1/30 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-3-30 1475-2875 https://doaj.org/article/ec73661e164a4e48a0efa3dbdd0fd225 Malaria Journal, Vol 3, Iss 1, p 30 (2004) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2004 ftdoajarticles https://doi.org/10.1186/1475-2875-3-30 2022-12-31T08:50:27Z Abstract Background Both host and pathogen factors contribute to disease outcome in Plasmodium falciparum infection. The feasibility of studying the P. falciparum in vivo transcriptome to understand parasite transcriptional response while it resides in the human host is presented. Methods A custom made oligonucleotide array with probes based on the P. falciparum 3D7 laboratory strain chromosome 2 sequence was used to detect in vivo P. falciparum transcripts. This study analyzed transcripts from total RNA derived from small blood samples of P. falciparum infected patients and compared the in vivo expression profile to the in vitro cultivated 3D7 strain transcriptome. Results The data demonstrated that in vivo transcription can be studied from a small blood sample, despite the abundance of human RNA. The in vivo transcriptome is similar to the 3D7 ring stage transcriptome, but there are significant differences in genes encoding a sexual stage antigen and surface proteins. Conclusions Whole genome transcription analysis of P. falciparum can be carried out successfully and further studies in selected patient cohorts may provide insight into parasite in vivo biology and defense against host immunity. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 3 1 30 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Sultan Ali Mboup Soulyemane Ndir Omar Zhou Yingyao Fang Xuemin Sarr Ousmane Le Roch Karine G Daily Johanna P Winzeler Elizabeth A Wirth Dyann F In vivo transcriptional profiling of Plasmodium falciparum |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Both host and pathogen factors contribute to disease outcome in Plasmodium falciparum infection. The feasibility of studying the P. falciparum in vivo transcriptome to understand parasite transcriptional response while it resides in the human host is presented. Methods A custom made oligonucleotide array with probes based on the P. falciparum 3D7 laboratory strain chromosome 2 sequence was used to detect in vivo P. falciparum transcripts. This study analyzed transcripts from total RNA derived from small blood samples of P. falciparum infected patients and compared the in vivo expression profile to the in vitro cultivated 3D7 strain transcriptome. Results The data demonstrated that in vivo transcription can be studied from a small blood sample, despite the abundance of human RNA. The in vivo transcriptome is similar to the 3D7 ring stage transcriptome, but there are significant differences in genes encoding a sexual stage antigen and surface proteins. Conclusions Whole genome transcription analysis of P. falciparum can be carried out successfully and further studies in selected patient cohorts may provide insight into parasite in vivo biology and defense against host immunity. |
format |
Article in Journal/Newspaper |
author |
Sultan Ali Mboup Soulyemane Ndir Omar Zhou Yingyao Fang Xuemin Sarr Ousmane Le Roch Karine G Daily Johanna P Winzeler Elizabeth A Wirth Dyann F |
author_facet |
Sultan Ali Mboup Soulyemane Ndir Omar Zhou Yingyao Fang Xuemin Sarr Ousmane Le Roch Karine G Daily Johanna P Winzeler Elizabeth A Wirth Dyann F |
author_sort |
Sultan Ali |
title |
In vivo transcriptional profiling of Plasmodium falciparum |
title_short |
In vivo transcriptional profiling of Plasmodium falciparum |
title_full |
In vivo transcriptional profiling of Plasmodium falciparum |
title_fullStr |
In vivo transcriptional profiling of Plasmodium falciparum |
title_full_unstemmed |
In vivo transcriptional profiling of Plasmodium falciparum |
title_sort |
in vivo transcriptional profiling of plasmodium falciparum |
publisher |
BMC |
publishDate |
2004 |
url |
https://doi.org/10.1186/1475-2875-3-30 https://doaj.org/article/ec73661e164a4e48a0efa3dbdd0fd225 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 3, Iss 1, p 30 (2004) |
op_relation |
http://www.malariajournal.com/content/3/1/30 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-3-30 1475-2875 https://doaj.org/article/ec73661e164a4e48a0efa3dbdd0fd225 |
op_doi |
https://doi.org/10.1186/1475-2875-3-30 |
container_title |
Malaria Journal |
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3 |
container_issue |
1 |
container_start_page |
30 |
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1766337893975982080 |