Recombinant Expression and Characterization of a Novel Thermo-Alkaline Lipase with Increased Solvent Stability from the Antarctic Thermophilic Bacterium Geobacillus sp. ID17

• Published in: International Journal of Molecular Sciences
• Main Authors: Diego Salas-Bruggink, Hardy Guzmán, Giannina Espina, Jenny M. Blamey
• Format: Article in Journal/Newspaper
• Language: English
• Published: MDPI AG 2024
• Subjects: esterase; protein purification; biocatalyst; biotechnology; extremophile; extremozyme; Biology (General); QH301-705.5; Chemistry; QD1-999; Antarctic; Deception Island; The Antarctic; Antarc*; Antarctica
• Online Access: https://doi.org/10.3390/ijms25147928; https://doaj.org/article/ec6edc6eb8dc41dcb58093a7c9b4e686

Lipases are enzymes that hydrolyze long-chain carboxylic esters, and in the presence of organic solvents, they catalyze organic synthesis reactions. However, the use of solvents in these processes often results in enzyme denaturation, leading to a reduction in enzymatic activity. Consequently, there...