Ultrastructural study on the morphological changes to male worms of Schistosoma mansoni after in vitro exposure to allicin
INTRODUCTION: Garlic has a wide range of actions, including antibacterial, antiviral, antifungal, antiprotozoal and anthelmintic actions. This antiparasitic activity has been attributed to allicin, which is the main constituent of garlic. The present study aimed to investigate the in vitro activity...
Published in: | Revista da Sociedade Brasileira de Medicina Tropical |
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Sociedade Brasileira de Medicina Tropical (SBMT)
2011
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Online Access: | https://doi.org/10.1590/s0037-86822011005000023 https://doaj.org/article/ec404fb16e84496fb6dd61dc758e7645 |
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ftdoajarticles:oai:doaj.org/article:ec404fb16e84496fb6dd61dc758e7645 2023-05-15T15:11:01+02:00 Ultrastructural study on the morphological changes to male worms of Schistosoma mansoni after in vitro exposure to allicin Caliandra Maria Bezerra Luna Lima Francisca Inês de Sousa Freitas Liana Clébia Soares Lima de Morais Marília Gabriela dos Santos Cavalcanti Lânia Ferreira da Silva Rafael José Ribeiro Padilha Constância Gayoso Simões Barbosa Fábio André Brayner dos Santos Luiz Carlos Alves Margareth de Fátima Formiga Melo Diniz 2011-06-01T00:00:00Z https://doi.org/10.1590/s0037-86822011005000023 https://doaj.org/article/ec404fb16e84496fb6dd61dc758e7645 EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822011000300013&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/s0037-86822011005000023 https://doaj.org/article/ec404fb16e84496fb6dd61dc758e7645 Revista da Sociedade Brasileira de Medicina Tropical, Vol 44, Iss 3, Pp 327-330 (2011) Alho Schistosoma mansoni Microscopia eletrônica de varredura Arctic medicine. Tropical medicine RC955-962 article 2011 ftdoajarticles https://doi.org/10.1590/s0037-86822011005000023 2022-12-31T02:53:12Z INTRODUCTION: Garlic has a wide range of actions, including antibacterial, antiviral, antifungal, antiprotozoal and anthelmintic actions. This antiparasitic activity has been attributed to allicin, which is the main constituent of garlic. The present study aimed to investigate the in vitro activity of allicin on the tegument of adult Schistosoma mansoni worms using scanning electron microscopy. METHODS: Swiss Webster mice were infected with S. mansoni cercariae (100 per mouse) and sacrificed 50 days later to acquire the adult worms. These worms were collected by perfusion and placed in RPMI medium 1,640 at 37°C before transferring to RPMI media containing 0 (control), 5, 10, 15 and 20mg/mL of allicin, where they were incubated for 2h. The worms were fixed in 2.5% glutaraldehyde solution, washed twice, post-fixed in osmium tetroxide, washed twice and then dehydrated with ascending grades of ethanol. The samples were air-dried, mounted on stubs, gold coated in an ion sputtering unit and viewed using a scanning electron microscope. RESULTS: A concentration of 5mg/mL caused wrinkling in the tegument; a concentration of 10mg/mL resulted in changes to tubercles and loss or modification of spines. With 15 and 20mg/mL increasing damage to the tegument could be seen, such as vesicle formation and the presence of ulcers. CONCLUSIONS: These findings demonstrate the effect of allicin on adult S. mansoni worms and indicate that most of the changes occur at concentrations greater than that normally indicated for treatment. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista da Sociedade Brasileira de Medicina Tropical 44 3 327 330 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Alho Schistosoma mansoni Microscopia eletrônica de varredura Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
Alho Schistosoma mansoni Microscopia eletrônica de varredura Arctic medicine. Tropical medicine RC955-962 Caliandra Maria Bezerra Luna Lima Francisca Inês de Sousa Freitas Liana Clébia Soares Lima de Morais Marília Gabriela dos Santos Cavalcanti Lânia Ferreira da Silva Rafael José Ribeiro Padilha Constância Gayoso Simões Barbosa Fábio André Brayner dos Santos Luiz Carlos Alves Margareth de Fátima Formiga Melo Diniz Ultrastructural study on the morphological changes to male worms of Schistosoma mansoni after in vitro exposure to allicin |
topic_facet |
Alho Schistosoma mansoni Microscopia eletrônica de varredura Arctic medicine. Tropical medicine RC955-962 |
description |
INTRODUCTION: Garlic has a wide range of actions, including antibacterial, antiviral, antifungal, antiprotozoal and anthelmintic actions. This antiparasitic activity has been attributed to allicin, which is the main constituent of garlic. The present study aimed to investigate the in vitro activity of allicin on the tegument of adult Schistosoma mansoni worms using scanning electron microscopy. METHODS: Swiss Webster mice were infected with S. mansoni cercariae (100 per mouse) and sacrificed 50 days later to acquire the adult worms. These worms were collected by perfusion and placed in RPMI medium 1,640 at 37°C before transferring to RPMI media containing 0 (control), 5, 10, 15 and 20mg/mL of allicin, where they were incubated for 2h. The worms were fixed in 2.5% glutaraldehyde solution, washed twice, post-fixed in osmium tetroxide, washed twice and then dehydrated with ascending grades of ethanol. The samples were air-dried, mounted on stubs, gold coated in an ion sputtering unit and viewed using a scanning electron microscope. RESULTS: A concentration of 5mg/mL caused wrinkling in the tegument; a concentration of 10mg/mL resulted in changes to tubercles and loss or modification of spines. With 15 and 20mg/mL increasing damage to the tegument could be seen, such as vesicle formation and the presence of ulcers. CONCLUSIONS: These findings demonstrate the effect of allicin on adult S. mansoni worms and indicate that most of the changes occur at concentrations greater than that normally indicated for treatment. |
format |
Article in Journal/Newspaper |
author |
Caliandra Maria Bezerra Luna Lima Francisca Inês de Sousa Freitas Liana Clébia Soares Lima de Morais Marília Gabriela dos Santos Cavalcanti Lânia Ferreira da Silva Rafael José Ribeiro Padilha Constância Gayoso Simões Barbosa Fábio André Brayner dos Santos Luiz Carlos Alves Margareth de Fátima Formiga Melo Diniz |
author_facet |
Caliandra Maria Bezerra Luna Lima Francisca Inês de Sousa Freitas Liana Clébia Soares Lima de Morais Marília Gabriela dos Santos Cavalcanti Lânia Ferreira da Silva Rafael José Ribeiro Padilha Constância Gayoso Simões Barbosa Fábio André Brayner dos Santos Luiz Carlos Alves Margareth de Fátima Formiga Melo Diniz |
author_sort |
Caliandra Maria Bezerra Luna Lima |
title |
Ultrastructural study on the morphological changes to male worms of Schistosoma mansoni after in vitro exposure to allicin |
title_short |
Ultrastructural study on the morphological changes to male worms of Schistosoma mansoni after in vitro exposure to allicin |
title_full |
Ultrastructural study on the morphological changes to male worms of Schistosoma mansoni after in vitro exposure to allicin |
title_fullStr |
Ultrastructural study on the morphological changes to male worms of Schistosoma mansoni after in vitro exposure to allicin |
title_full_unstemmed |
Ultrastructural study on the morphological changes to male worms of Schistosoma mansoni after in vitro exposure to allicin |
title_sort |
ultrastructural study on the morphological changes to male worms of schistosoma mansoni after in vitro exposure to allicin |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
publishDate |
2011 |
url |
https://doi.org/10.1590/s0037-86822011005000023 https://doaj.org/article/ec404fb16e84496fb6dd61dc758e7645 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 44, Iss 3, Pp 327-330 (2011) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822011000300013&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/s0037-86822011005000023 https://doaj.org/article/ec404fb16e84496fb6dd61dc758e7645 |
op_doi |
https://doi.org/10.1590/s0037-86822011005000023 |
container_title |
Revista da Sociedade Brasileira de Medicina Tropical |
container_volume |
44 |
container_issue |
3 |
container_start_page |
327 |
op_container_end_page |
330 |
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1766341933258506240 |