Fungal and fungal-like diversity in marine sediments from the maritime Antarctic assessed using DNA metabarcoding
Abstract We assessed the fungal and fungal-like sequence diversity present in marine sediments obtained in the vicinity of the South Shetland Islands (Southern Ocean) using DNA metabarcoding through high-throughput sequencing (HTS). A total of 193,436 DNA reads were detected in sediment obtained fro...
Published in: | Scientific Reports |
---|---|
Main Authors: | , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Nature Portfolio
2022
|
Subjects: | |
Online Access: | https://doi.org/10.1038/s41598-022-25310-2 https://doaj.org/article/ebd8227409404f959c03f964d6055a97 |
id |
ftdoajarticles:oai:doaj.org/article:ebd8227409404f959c03f964d6055a97 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:ebd8227409404f959c03f964d6055a97 2023-05-15T14:01:43+02:00 Fungal and fungal-like diversity in marine sediments from the maritime Antarctic assessed using DNA metabarcoding Mayanne Karla da Silva Láuren Machado Drumond de Souza Rosemary Vieira Arthur Ayres Neto Fabyano A. C. Lopes Fábio S. de Oliveira Peter Convey Micheline Carvalho-Silva Alysson Wagner Fernandes Duarte Paulo E. A. S. Câmara Luiz Henrique Rosa 2022-12-01T00:00:00Z https://doi.org/10.1038/s41598-022-25310-2 https://doaj.org/article/ebd8227409404f959c03f964d6055a97 EN eng Nature Portfolio https://doi.org/10.1038/s41598-022-25310-2 https://doaj.org/toc/2045-2322 doi:10.1038/s41598-022-25310-2 2045-2322 https://doaj.org/article/ebd8227409404f959c03f964d6055a97 Scientific Reports, Vol 12, Iss 1, Pp 1-10 (2022) Medicine R Science Q article 2022 ftdoajarticles https://doi.org/10.1038/s41598-022-25310-2 2022-12-30T19:34:58Z Abstract We assessed the fungal and fungal-like sequence diversity present in marine sediments obtained in the vicinity of the South Shetland Islands (Southern Ocean) using DNA metabarcoding through high-throughput sequencing (HTS). A total of 193,436 DNA reads were detected in sediment obtained from three locations: Walker Bay (Livingston Island) at 52 m depth (48,112 reads), Whalers Bay (Deception Island) at 151 m (104,704) and English Strait at 404 m (40,620). The DNA sequence reads were assigned to 133 distinct fungal amplicon sequence variants (ASVs) representing the phyla Ascomycota, Basidiomycota, Mortierellomycota, Chytridiomycota, Glomeromycota, Monoblepharomycota, Mucoromycota and Rozellomycota and the fungal-like Straminopila. Thelebolus balaustiformis, Pseudogymnoascus sp., Fungi sp. 1, Ciliophora sp., Agaricomycetes sp. and Chaetoceros sp. were the dominant assigned taxa. Thirty-eight fungal ASVs could only be assigned to higher taxonomic levels, and may represent taxa not currently included in the available databases or represent new taxa and/or new records for Antarctica. The total fungal community displayed high indices of diversity, richness and moderate to low dominance. However, diversity and taxa distribution varied across the three sampling sites. In Walker Bay, unidentified fungi were dominant in the sequence assemblage. Whalers Bay sediment was dominated by Antarctic endemic and cold-adapted taxa. Sediment from English Strait was dominated by Ciliophora sp. and Chaetoceros sp. These fungal assemblages were dominated by saprotrophic, plant and animal pathogenic and symbiotic taxa. The detection of an apparently rich and diverse fungal community in these marine sediments reinforces the need for further studies to characterize their richness, functional ecology and potential biotechnological applications. Article in Journal/Newspaper Antarc* Antarctic Antarctica Deception Island Livingston Island South Shetland Islands Southern Ocean Walker Bay Directory of Open Access Journals: DOAJ Articles Antarctic Southern Ocean South Shetland Islands Deception Island ENVELOPE(-60.633,-60.633,-62.950,-62.950) Livingston Island ENVELOPE(-60.500,-60.500,-62.600,-62.600) Whalers Bay ENVELOPE(69.917,69.917,-49.100,-49.100) Walker Bay ENVELOPE(-60.700,-60.700,-62.633,-62.633) English Strait ENVELOPE(-59.633,-59.633,-62.450,-62.450) Scientific Reports 12 1 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Medicine R Science Q |
spellingShingle |
Medicine R Science Q Mayanne Karla da Silva Láuren Machado Drumond de Souza Rosemary Vieira Arthur Ayres Neto Fabyano A. C. Lopes Fábio S. de Oliveira Peter Convey Micheline Carvalho-Silva Alysson Wagner Fernandes Duarte Paulo E. A. S. Câmara Luiz Henrique Rosa Fungal and fungal-like diversity in marine sediments from the maritime Antarctic assessed using DNA metabarcoding |
topic_facet |
Medicine R Science Q |
description |
Abstract We assessed the fungal and fungal-like sequence diversity present in marine sediments obtained in the vicinity of the South Shetland Islands (Southern Ocean) using DNA metabarcoding through high-throughput sequencing (HTS). A total of 193,436 DNA reads were detected in sediment obtained from three locations: Walker Bay (Livingston Island) at 52 m depth (48,112 reads), Whalers Bay (Deception Island) at 151 m (104,704) and English Strait at 404 m (40,620). The DNA sequence reads were assigned to 133 distinct fungal amplicon sequence variants (ASVs) representing the phyla Ascomycota, Basidiomycota, Mortierellomycota, Chytridiomycota, Glomeromycota, Monoblepharomycota, Mucoromycota and Rozellomycota and the fungal-like Straminopila. Thelebolus balaustiformis, Pseudogymnoascus sp., Fungi sp. 1, Ciliophora sp., Agaricomycetes sp. and Chaetoceros sp. were the dominant assigned taxa. Thirty-eight fungal ASVs could only be assigned to higher taxonomic levels, and may represent taxa not currently included in the available databases or represent new taxa and/or new records for Antarctica. The total fungal community displayed high indices of diversity, richness and moderate to low dominance. However, diversity and taxa distribution varied across the three sampling sites. In Walker Bay, unidentified fungi were dominant in the sequence assemblage. Whalers Bay sediment was dominated by Antarctic endemic and cold-adapted taxa. Sediment from English Strait was dominated by Ciliophora sp. and Chaetoceros sp. These fungal assemblages were dominated by saprotrophic, plant and animal pathogenic and symbiotic taxa. The detection of an apparently rich and diverse fungal community in these marine sediments reinforces the need for further studies to characterize their richness, functional ecology and potential biotechnological applications. |
format |
Article in Journal/Newspaper |
author |
Mayanne Karla da Silva Láuren Machado Drumond de Souza Rosemary Vieira Arthur Ayres Neto Fabyano A. C. Lopes Fábio S. de Oliveira Peter Convey Micheline Carvalho-Silva Alysson Wagner Fernandes Duarte Paulo E. A. S. Câmara Luiz Henrique Rosa |
author_facet |
Mayanne Karla da Silva Láuren Machado Drumond de Souza Rosemary Vieira Arthur Ayres Neto Fabyano A. C. Lopes Fábio S. de Oliveira Peter Convey Micheline Carvalho-Silva Alysson Wagner Fernandes Duarte Paulo E. A. S. Câmara Luiz Henrique Rosa |
author_sort |
Mayanne Karla da Silva |
title |
Fungal and fungal-like diversity in marine sediments from the maritime Antarctic assessed using DNA metabarcoding |
title_short |
Fungal and fungal-like diversity in marine sediments from the maritime Antarctic assessed using DNA metabarcoding |
title_full |
Fungal and fungal-like diversity in marine sediments from the maritime Antarctic assessed using DNA metabarcoding |
title_fullStr |
Fungal and fungal-like diversity in marine sediments from the maritime Antarctic assessed using DNA metabarcoding |
title_full_unstemmed |
Fungal and fungal-like diversity in marine sediments from the maritime Antarctic assessed using DNA metabarcoding |
title_sort |
fungal and fungal-like diversity in marine sediments from the maritime antarctic assessed using dna metabarcoding |
publisher |
Nature Portfolio |
publishDate |
2022 |
url |
https://doi.org/10.1038/s41598-022-25310-2 https://doaj.org/article/ebd8227409404f959c03f964d6055a97 |
long_lat |
ENVELOPE(-60.633,-60.633,-62.950,-62.950) ENVELOPE(-60.500,-60.500,-62.600,-62.600) ENVELOPE(69.917,69.917,-49.100,-49.100) ENVELOPE(-60.700,-60.700,-62.633,-62.633) ENVELOPE(-59.633,-59.633,-62.450,-62.450) |
geographic |
Antarctic Southern Ocean South Shetland Islands Deception Island Livingston Island Whalers Bay Walker Bay English Strait |
geographic_facet |
Antarctic Southern Ocean South Shetland Islands Deception Island Livingston Island Whalers Bay Walker Bay English Strait |
genre |
Antarc* Antarctic Antarctica Deception Island Livingston Island South Shetland Islands Southern Ocean Walker Bay |
genre_facet |
Antarc* Antarctic Antarctica Deception Island Livingston Island South Shetland Islands Southern Ocean Walker Bay |
op_source |
Scientific Reports, Vol 12, Iss 1, Pp 1-10 (2022) |
op_relation |
https://doi.org/10.1038/s41598-022-25310-2 https://doaj.org/toc/2045-2322 doi:10.1038/s41598-022-25310-2 2045-2322 https://doaj.org/article/ebd8227409404f959c03f964d6055a97 |
op_doi |
https://doi.org/10.1038/s41598-022-25310-2 |
container_title |
Scientific Reports |
container_volume |
12 |
container_issue |
1 |
_version_ |
1766271762599772160 |