Comparative analysis of European bat lyssavirus 1 pathogenicity in the mouse model.
European bat lyssavirus 1 is responsible for most bat rabies cases in Europe. Although EBLV-1 isolates display a high degree of sequence identity, different sublineages exist. In individual isolates various insertions and deletions have been identified, with unknown impact on viral replication and p...
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ftdoajarticles:oai:doaj.org/article:ebb9014b4a9d4fbd9bbc8bdb96628dd2 2023-05-15T15:07:20+02:00 Comparative analysis of European bat lyssavirus 1 pathogenicity in the mouse model. Elisa Eggerbauer Florian Pfaff Stefan Finke Dirk Höper Martin Beer Thomas C Mettenleiter Tobias Nolden Jens-Peter Teifke Thomas Müller Conrad M Freuling 2017-06-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0005668 https://doaj.org/article/ebb9014b4a9d4fbd9bbc8bdb96628dd2 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5491315?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005668 https://doaj.org/article/ebb9014b4a9d4fbd9bbc8bdb96628dd2 PLoS Neglected Tropical Diseases, Vol 11, Iss 6, p e0005668 (2017) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2017 ftdoajarticles https://doi.org/10.1371/journal.pntd.0005668 2022-12-30T21:59:56Z European bat lyssavirus 1 is responsible for most bat rabies cases in Europe. Although EBLV-1 isolates display a high degree of sequence identity, different sublineages exist. In individual isolates various insertions and deletions have been identified, with unknown impact on viral replication and pathogenicity. In order to assess whether different genetic features of EBLV-1 isolates correlate with phenotypic changes, different EBLV-1 variants were compared for pathogenicity in the mouse model. Groups of three mice were infected intracranially (i.c.) with 102 TCID50/ml and groups of six mice were infected intramuscularly (i.m.) with 105 TCID50/ml and 102 TCID50/ml as well as intranasally (i.n.) with 102 TCID50/ml. Significant differences in survival following i.m. inoculation with low doses as well as i.n. inoculation were observed. Also, striking variations in incubation periods following i.c. inoculation and i.m. inoculation with high doses were seen. Hereby, the clinical picture differed between general symptoms, spasms and aggressiveness depending on the inoculation route. Immunohistochemistry of mouse brains showed that the virus distribution in the brain depended on the inoculation route. In conclusion, different EBLV-1 isolates differ in pathogenicity indicating variation which is not reflected in studies of single isolates. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 11 6 e0005668 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Elisa Eggerbauer Florian Pfaff Stefan Finke Dirk Höper Martin Beer Thomas C Mettenleiter Tobias Nolden Jens-Peter Teifke Thomas Müller Conrad M Freuling Comparative analysis of European bat lyssavirus 1 pathogenicity in the mouse model. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
European bat lyssavirus 1 is responsible for most bat rabies cases in Europe. Although EBLV-1 isolates display a high degree of sequence identity, different sublineages exist. In individual isolates various insertions and deletions have been identified, with unknown impact on viral replication and pathogenicity. In order to assess whether different genetic features of EBLV-1 isolates correlate with phenotypic changes, different EBLV-1 variants were compared for pathogenicity in the mouse model. Groups of three mice were infected intracranially (i.c.) with 102 TCID50/ml and groups of six mice were infected intramuscularly (i.m.) with 105 TCID50/ml and 102 TCID50/ml as well as intranasally (i.n.) with 102 TCID50/ml. Significant differences in survival following i.m. inoculation with low doses as well as i.n. inoculation were observed. Also, striking variations in incubation periods following i.c. inoculation and i.m. inoculation with high doses were seen. Hereby, the clinical picture differed between general symptoms, spasms and aggressiveness depending on the inoculation route. Immunohistochemistry of mouse brains showed that the virus distribution in the brain depended on the inoculation route. In conclusion, different EBLV-1 isolates differ in pathogenicity indicating variation which is not reflected in studies of single isolates. |
format |
Article in Journal/Newspaper |
author |
Elisa Eggerbauer Florian Pfaff Stefan Finke Dirk Höper Martin Beer Thomas C Mettenleiter Tobias Nolden Jens-Peter Teifke Thomas Müller Conrad M Freuling |
author_facet |
Elisa Eggerbauer Florian Pfaff Stefan Finke Dirk Höper Martin Beer Thomas C Mettenleiter Tobias Nolden Jens-Peter Teifke Thomas Müller Conrad M Freuling |
author_sort |
Elisa Eggerbauer |
title |
Comparative analysis of European bat lyssavirus 1 pathogenicity in the mouse model. |
title_short |
Comparative analysis of European bat lyssavirus 1 pathogenicity in the mouse model. |
title_full |
Comparative analysis of European bat lyssavirus 1 pathogenicity in the mouse model. |
title_fullStr |
Comparative analysis of European bat lyssavirus 1 pathogenicity in the mouse model. |
title_full_unstemmed |
Comparative analysis of European bat lyssavirus 1 pathogenicity in the mouse model. |
title_sort |
comparative analysis of european bat lyssavirus 1 pathogenicity in the mouse model. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2017 |
url |
https://doi.org/10.1371/journal.pntd.0005668 https://doaj.org/article/ebb9014b4a9d4fbd9bbc8bdb96628dd2 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 11, Iss 6, p e0005668 (2017) |
op_relation |
http://europepmc.org/articles/PMC5491315?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005668 https://doaj.org/article/ebb9014b4a9d4fbd9bbc8bdb96628dd2 |
op_doi |
https://doi.org/10.1371/journal.pntd.0005668 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
11 |
container_issue |
6 |
container_start_page |
e0005668 |
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