Echinococcus granulosus sensu lato GENOTYPES IN DOMESTIC LIVESTOCK AND HUMANS IN GOLESTAN PROVINCE, IRAN
Cystic echinococcosis (CE) is a globally parasitic zoonosis caused by larval stages of Echinococcus granulosus. This study investigated E. granulosus genotypes isolated from livestock and humans in the Golestan province, northern Iran, southeast of the Caspian sea, using partial sequencing data of t...
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ftdoajarticles:oai:doaj.org/article:ebb42f10b62046c4ae0f655e509c496f 2024-09-09T19:26:23+00:00 Echinococcus granulosus sensu lato GENOTYPES IN DOMESTIC LIVESTOCK AND HUMANS IN GOLESTAN PROVINCE, IRAN Mitra SHARBATKHORI Asal TANZIFI Sima ROSTAMI Masoomeh ROSTAMI Majid FASIHI HARANDI 2016-01-01T00:00:00Z https://doi.org/10.1590/S1678-9946201658038 https://doaj.org/article/ebb42f10b62046c4ae0f655e509c496f EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652016005000228&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/S1678-9946201658038 https://doaj.org/article/ebb42f10b62046c4ae0f655e509c496f Revista do Instituto de Medicina Tropical de São Paulo, Vol 58, Iss 0 (2016) Echinococcus granulosus Genotyping cox1 nad1 Iran Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2016 ftdoajarticles https://doi.org/10.1590/S1678-9946201658038 2024-08-05T17:49:30Z Cystic echinococcosis (CE) is a globally parasitic zoonosis caused by larval stages of Echinococcus granulosus. This study investigated E. granulosus genotypes isolated from livestock and humans in the Golestan province, northern Iran, southeast of the Caspian sea, using partial sequencing data of the cytochrome c oxidase subunit 1 (cox1) and NADH dehydrogenase 1 (nad1) mitochondrial genes. Seventy E. granulosus isolates were collected from animals in slaughterhouses: 18 isolates from sheep, 40 from cattle, nine from camels, two from buffaloes and one from a goat, along with four human isolates (formalin-fixed, paraffin-embedded tissues) from CE patients of provincial hospitals. All isolates were successfully analysed by PCR amplification and sequencing. The sequence analysis found four E. granulosus genotypes among the 74 CE isolates: G1 (78.3%), G2 (2.7%), G3 (15%) and G6 (4%). The G1-G3 complex genotype was found in all of the sheep, goat, cattle and buffalo isolates. Among the nine camel isolates, the frequency of G1-G3 and G6 genotypes were 66.7% and 33.3%, respectively. All four human CE isolates belonged to E. granulosus sensu stricto. This study reports the first occurrence of the G2 genotype in cattle from Iran and confirms the previously reported G3 genotype in camels in the same country. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista do Instituto de Medicina Tropical de São Paulo 58 0 |
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English |
topic |
Echinococcus granulosus Genotyping cox1 nad1 Iran Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Echinococcus granulosus Genotyping cox1 nad1 Iran Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Mitra SHARBATKHORI Asal TANZIFI Sima ROSTAMI Masoomeh ROSTAMI Majid FASIHI HARANDI Echinococcus granulosus sensu lato GENOTYPES IN DOMESTIC LIVESTOCK AND HUMANS IN GOLESTAN PROVINCE, IRAN |
topic_facet |
Echinococcus granulosus Genotyping cox1 nad1 Iran Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Cystic echinococcosis (CE) is a globally parasitic zoonosis caused by larval stages of Echinococcus granulosus. This study investigated E. granulosus genotypes isolated from livestock and humans in the Golestan province, northern Iran, southeast of the Caspian sea, using partial sequencing data of the cytochrome c oxidase subunit 1 (cox1) and NADH dehydrogenase 1 (nad1) mitochondrial genes. Seventy E. granulosus isolates were collected from animals in slaughterhouses: 18 isolates from sheep, 40 from cattle, nine from camels, two from buffaloes and one from a goat, along with four human isolates (formalin-fixed, paraffin-embedded tissues) from CE patients of provincial hospitals. All isolates were successfully analysed by PCR amplification and sequencing. The sequence analysis found four E. granulosus genotypes among the 74 CE isolates: G1 (78.3%), G2 (2.7%), G3 (15%) and G6 (4%). The G1-G3 complex genotype was found in all of the sheep, goat, cattle and buffalo isolates. Among the nine camel isolates, the frequency of G1-G3 and G6 genotypes were 66.7% and 33.3%, respectively. All four human CE isolates belonged to E. granulosus sensu stricto. This study reports the first occurrence of the G2 genotype in cattle from Iran and confirms the previously reported G3 genotype in camels in the same country. |
format |
Article in Journal/Newspaper |
author |
Mitra SHARBATKHORI Asal TANZIFI Sima ROSTAMI Masoomeh ROSTAMI Majid FASIHI HARANDI |
author_facet |
Mitra SHARBATKHORI Asal TANZIFI Sima ROSTAMI Masoomeh ROSTAMI Majid FASIHI HARANDI |
author_sort |
Mitra SHARBATKHORI |
title |
Echinococcus granulosus sensu lato GENOTYPES IN DOMESTIC LIVESTOCK AND HUMANS IN GOLESTAN PROVINCE, IRAN |
title_short |
Echinococcus granulosus sensu lato GENOTYPES IN DOMESTIC LIVESTOCK AND HUMANS IN GOLESTAN PROVINCE, IRAN |
title_full |
Echinococcus granulosus sensu lato GENOTYPES IN DOMESTIC LIVESTOCK AND HUMANS IN GOLESTAN PROVINCE, IRAN |
title_fullStr |
Echinococcus granulosus sensu lato GENOTYPES IN DOMESTIC LIVESTOCK AND HUMANS IN GOLESTAN PROVINCE, IRAN |
title_full_unstemmed |
Echinococcus granulosus sensu lato GENOTYPES IN DOMESTIC LIVESTOCK AND HUMANS IN GOLESTAN PROVINCE, IRAN |
title_sort |
echinococcus granulosus sensu lato genotypes in domestic livestock and humans in golestan province, iran |
publisher |
Universidade de São Paulo (USP) |
publishDate |
2016 |
url |
https://doi.org/10.1590/S1678-9946201658038 https://doaj.org/article/ebb42f10b62046c4ae0f655e509c496f |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 58, Iss 0 (2016) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652016005000228&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/S1678-9946201658038 https://doaj.org/article/ebb42f10b62046c4ae0f655e509c496f |
op_doi |
https://doi.org/10.1590/S1678-9946201658038 |
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Revista do Instituto de Medicina Tropical de São Paulo |
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58 |
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