The microneme proteins CTRP and SOAP are not essential for Plasmodium berghei ookinete to oocyst transformation in vitro in a cell free system
Abstract Background Two Plasmodium berghei ookinete micronemal proteins, circumsporozoite and TRAP related protein (CTRP) and secreted ookinete adhesive protein (SOAP) both interact with the basal lamina component laminin. Following gene disruption studies it has been proposed that, apart from their...
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ftdoajarticles:oai:doaj.org/article:eb9d4be5cbed4fb6afe595a87443abde 2023-05-15T15:06:15+02:00 The microneme proteins CTRP and SOAP are not essential for Plasmodium berghei ookinete to oocyst transformation in vitro in a cell free system Hurd Hilary Underhill Ann Nacer Adéla 2008-05-01T00:00:00Z https://doi.org/10.1186/1475-2875-7-82 https://doaj.org/article/eb9d4be5cbed4fb6afe595a87443abde EN eng BMC http://www.malariajournal.com/content/7/1/82 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-7-82 1475-2875 https://doaj.org/article/eb9d4be5cbed4fb6afe595a87443abde Malaria Journal, Vol 7, Iss 1, p 82 (2008) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2008 ftdoajarticles https://doi.org/10.1186/1475-2875-7-82 2022-12-31T08:50:27Z Abstract Background Two Plasmodium berghei ookinete micronemal proteins, circumsporozoite and TRAP related protein (CTRP) and secreted ookinete adhesive protein (SOAP) both interact with the basal lamina component laminin. Following gene disruption studies it has been proposed that, apart from their role in motility, these proteins may be required for interactions leading to ookinete-to-oocyst transformation. Methods CTRP and SOAP null mutant P. berghei ookinetes were compared to P. berghei ANKA wild-type for their ability to transform and grow in vitro . To confirm in vitro findings for P . berghei CTRP-KO ookinetes were injected into the haemocoel of An opheles gambiae female mosquitoes. Results Transformation, growth, and viability were comparable for the gene disrupted and wild-type parasites. P . berghei CTRP-KO ookinetes were able to transform into oocysts in the haemocoel of An . gambiae mosquitoes. Conclusion Neither CTRP nor SOAP is required for parasite transformation in vitro . By-passing the midgut lumen allows for the transformation of P . berghei CTRP-KO ookinetes suggesting that it is not required for transformation in vivo . Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 7 1 82 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Hurd Hilary Underhill Ann Nacer Adéla The microneme proteins CTRP and SOAP are not essential for Plasmodium berghei ookinete to oocyst transformation in vitro in a cell free system |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Two Plasmodium berghei ookinete micronemal proteins, circumsporozoite and TRAP related protein (CTRP) and secreted ookinete adhesive protein (SOAP) both interact with the basal lamina component laminin. Following gene disruption studies it has been proposed that, apart from their role in motility, these proteins may be required for interactions leading to ookinete-to-oocyst transformation. Methods CTRP and SOAP null mutant P. berghei ookinetes were compared to P. berghei ANKA wild-type for their ability to transform and grow in vitro . To confirm in vitro findings for P . berghei CTRP-KO ookinetes were injected into the haemocoel of An opheles gambiae female mosquitoes. Results Transformation, growth, and viability were comparable for the gene disrupted and wild-type parasites. P . berghei CTRP-KO ookinetes were able to transform into oocysts in the haemocoel of An . gambiae mosquitoes. Conclusion Neither CTRP nor SOAP is required for parasite transformation in vitro . By-passing the midgut lumen allows for the transformation of P . berghei CTRP-KO ookinetes suggesting that it is not required for transformation in vivo . |
format |
Article in Journal/Newspaper |
author |
Hurd Hilary Underhill Ann Nacer Adéla |
author_facet |
Hurd Hilary Underhill Ann Nacer Adéla |
author_sort |
Hurd Hilary |
title |
The microneme proteins CTRP and SOAP are not essential for Plasmodium berghei ookinete to oocyst transformation in vitro in a cell free system |
title_short |
The microneme proteins CTRP and SOAP are not essential for Plasmodium berghei ookinete to oocyst transformation in vitro in a cell free system |
title_full |
The microneme proteins CTRP and SOAP are not essential for Plasmodium berghei ookinete to oocyst transformation in vitro in a cell free system |
title_fullStr |
The microneme proteins CTRP and SOAP are not essential for Plasmodium berghei ookinete to oocyst transformation in vitro in a cell free system |
title_full_unstemmed |
The microneme proteins CTRP and SOAP are not essential for Plasmodium berghei ookinete to oocyst transformation in vitro in a cell free system |
title_sort |
microneme proteins ctrp and soap are not essential for plasmodium berghei ookinete to oocyst transformation in vitro in a cell free system |
publisher |
BMC |
publishDate |
2008 |
url |
https://doi.org/10.1186/1475-2875-7-82 https://doaj.org/article/eb9d4be5cbed4fb6afe595a87443abde |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 7, Iss 1, p 82 (2008) |
op_relation |
http://www.malariajournal.com/content/7/1/82 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-7-82 1475-2875 https://doaj.org/article/eb9d4be5cbed4fb6afe595a87443abde |
op_doi |
https://doi.org/10.1186/1475-2875-7-82 |
container_title |
Malaria Journal |
container_volume |
7 |
container_issue |
1 |
container_start_page |
82 |
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1766337894136414208 |