Atomic Force Microscopy of host cell-amastigote interaction in cutaneous leishmaniasis
Abstract: Natural infection produced by the entry of promastigotes into the skin cells induces cutaneous leishmaniasis disease and the Interaction between phagocytic cells and different strains of Leishmania determine the course of disease in the mammalian host. In this study the cutaneous lesions f...
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Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales
2004
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ftdoajarticles:oai:doaj.org/article:eb8ae88dee594ab1b56beb8eeef09709 2023-10-01T03:54:20+02:00 Atomic Force Microscopy of host cell-amastigote interaction in cutaneous leishmaniasis Lugo de Yarbuh A Colasante C Alarcón M Moreno E 2004-12-01T00:00:00Z https://doaj.org/article/eb8ae88dee594ab1b56beb8eeef09709 EN ES eng spa Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales https://drive.google.com/file/d/1jGtqnIcyQ28wRw69boA4pEzSuE7edFlP/view https://doaj.org/toc/0075-5222 https://doaj.org/toc/2477-9628 0075-5222 2477-9628 https://doaj.org/article/eb8ae88dee594ab1b56beb8eeef09709 Kasmera, Vol 32, Iss 2, Pp 127-136 (2004) Microscopía de fuerza atómica Leishmania garnhami lesiones cutáneas amastigotes macrófagos linfocitos imagen 3D Atomic force microscopy amastigo- te macrophage lymphocyte 3D image Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2004 ftdoajarticles 2023-09-03T00:36:46Z Abstract: Natural infection produced by the entry of promastigotes into the skin cells induces cutaneous leishmaniasis disease and the Interaction between phagocytic cells and different strains of Leishmania determine the course of disease in the mammalian host. In this study the cutaneous lesions from hamsters infected with the L. garnhami were pressed on a microscope slide, allowed to dry and methanol-fixed in order to be ultrastructurally analyzed using atomic force microscopy (AFM). Free amastigotes showed their nucleus, kinetoplast and a depression of 5 microns, corresponding to the refractil organelle characteristic of this Leishmania. Parasites inside macrophages and lymphocytes and the topographical relationship with the host cytoplasm was also observed. Parasite-host-cell interaction revealed different membrane contact. The amastigote-macrophage contact is established by small macrophage filopodio associated with its cytoplasmic reduction near the contact site of 800 nm. The amastigote-lymphocyte contact shows fusion-like behavior between the two membranes without any cell specialization. The cutaneous lesion studied with AFM allowed observation with high resolution the close contact established between the parasite and its host cells as well as details of the fine structure of the amastigotes, applying a simple and rapid tissue preparation. Resumen: La infección natural producida por la entrada de promastigotes en la piel produce leishmaniasis cutánea y la interacción entre las células fagocíticas y las diferentes cepas de Leishmania determina el curso de la enfermedad en el hospedador vertebrado. En este estudio las lesiones cutáneas de hamsteres infectados con L. garnhami fueron frotadas sobre un portaobjeto, secadas a temperatura ambiente y fijadas con metanol para analizarlas ultraestructuralmente usando un microscopio de fuerza atómica (AFM). Los amastigotes mostraron el núcleo, kinetoplasto y una depresión de 5µ, correspondiente a la organela refráctil característica de esta especie. Los ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Entrada ENVELOPE(-60.552,-60.552,-62.998,-62.998) |
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Directory of Open Access Journals: DOAJ Articles |
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language |
English Spanish |
topic |
Microscopía de fuerza atómica Leishmania garnhami lesiones cutáneas amastigotes macrófagos linfocitos imagen 3D Atomic force microscopy amastigo- te macrophage lymphocyte 3D image Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Microscopía de fuerza atómica Leishmania garnhami lesiones cutáneas amastigotes macrófagos linfocitos imagen 3D Atomic force microscopy amastigo- te macrophage lymphocyte 3D image Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Lugo de Yarbuh A Colasante C Alarcón M Moreno E Atomic Force Microscopy of host cell-amastigote interaction in cutaneous leishmaniasis |
topic_facet |
Microscopía de fuerza atómica Leishmania garnhami lesiones cutáneas amastigotes macrófagos linfocitos imagen 3D Atomic force microscopy amastigo- te macrophage lymphocyte 3D image Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Abstract: Natural infection produced by the entry of promastigotes into the skin cells induces cutaneous leishmaniasis disease and the Interaction between phagocytic cells and different strains of Leishmania determine the course of disease in the mammalian host. In this study the cutaneous lesions from hamsters infected with the L. garnhami were pressed on a microscope slide, allowed to dry and methanol-fixed in order to be ultrastructurally analyzed using atomic force microscopy (AFM). Free amastigotes showed their nucleus, kinetoplast and a depression of 5 microns, corresponding to the refractil organelle characteristic of this Leishmania. Parasites inside macrophages and lymphocytes and the topographical relationship with the host cytoplasm was also observed. Parasite-host-cell interaction revealed different membrane contact. The amastigote-macrophage contact is established by small macrophage filopodio associated with its cytoplasmic reduction near the contact site of 800 nm. The amastigote-lymphocyte contact shows fusion-like behavior between the two membranes without any cell specialization. The cutaneous lesion studied with AFM allowed observation with high resolution the close contact established between the parasite and its host cells as well as details of the fine structure of the amastigotes, applying a simple and rapid tissue preparation. Resumen: La infección natural producida por la entrada de promastigotes en la piel produce leishmaniasis cutánea y la interacción entre las células fagocíticas y las diferentes cepas de Leishmania determina el curso de la enfermedad en el hospedador vertebrado. En este estudio las lesiones cutáneas de hamsteres infectados con L. garnhami fueron frotadas sobre un portaobjeto, secadas a temperatura ambiente y fijadas con metanol para analizarlas ultraestructuralmente usando un microscopio de fuerza atómica (AFM). Los amastigotes mostraron el núcleo, kinetoplasto y una depresión de 5µ, correspondiente a la organela refráctil característica de esta especie. Los ... |
format |
Article in Journal/Newspaper |
author |
Lugo de Yarbuh A Colasante C Alarcón M Moreno E |
author_facet |
Lugo de Yarbuh A Colasante C Alarcón M Moreno E |
author_sort |
Lugo de Yarbuh A |
title |
Atomic Force Microscopy of host cell-amastigote interaction in cutaneous leishmaniasis |
title_short |
Atomic Force Microscopy of host cell-amastigote interaction in cutaneous leishmaniasis |
title_full |
Atomic Force Microscopy of host cell-amastigote interaction in cutaneous leishmaniasis |
title_fullStr |
Atomic Force Microscopy of host cell-amastigote interaction in cutaneous leishmaniasis |
title_full_unstemmed |
Atomic Force Microscopy of host cell-amastigote interaction in cutaneous leishmaniasis |
title_sort |
atomic force microscopy of host cell-amastigote interaction in cutaneous leishmaniasis |
publisher |
Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales |
publishDate |
2004 |
url |
https://doaj.org/article/eb8ae88dee594ab1b56beb8eeef09709 |
long_lat |
ENVELOPE(-60.552,-60.552,-62.998,-62.998) |
geographic |
Arctic Entrada |
geographic_facet |
Arctic Entrada |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Kasmera, Vol 32, Iss 2, Pp 127-136 (2004) |
op_relation |
https://drive.google.com/file/d/1jGtqnIcyQ28wRw69boA4pEzSuE7edFlP/view https://doaj.org/toc/0075-5222 https://doaj.org/toc/2477-9628 0075-5222 2477-9628 https://doaj.org/article/eb8ae88dee594ab1b56beb8eeef09709 |
_version_ |
1778521838482096128 |