Enterotoxin and Biofilm Production in Clinical Isolates of Methicillin-Resistant Staphylococcus aureus
S. aureus has become a public health problem, due to the difficulty of treating infections caused by methicillin-resistant S. aureus (MRSA). The purpose of this research was to determine the production of enterotoxins A, B, C and D and the production of biofilm in clinical isolates of MRSA. Fifty MR...
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Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales
2014
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ftdoajarticles:oai:doaj.org/article:eb78217179164b63ba4bae95c9b270f4 2023-10-01T03:54:06+02:00 Enterotoxin and Biofilm Production in Clinical Isolates of Methicillin-Resistant Staphylococcus aureus Yeiny Ávila R. Messaria Ginestre P. Kutchynskaya Valero L. Maribel Castellano G. Sonia Romero A. Alfredo López Gresleida Rincón V. Lisette Sandrea T. 2014-08-01T00:00:00Z https://doaj.org/article/eb78217179164b63ba4bae95c9b270f4 EN ES eng spa Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales https://produccioncientificaluz.org/index.php/kasmera/article/view/19498 https://doaj.org/toc/0075-5222 https://doaj.org/toc/2477-9628 0075-5222 2477-9628 https://doaj.org/article/eb78217179164b63ba4bae95c9b270f4 Kasmera, Vol 42, Iss 2, Pp 105-115 (2014) S. Aureus Resistente a Meticilina Enteroto Xinas Biofilm Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2014 ftdoajarticles 2023-09-03T00:47:40Z S. aureus has become a public health problem, due to the difficulty of treating infections caused by methicillin-resistant S. aureus (MRSA). The purpose of this research was to determine the production of enterotoxins A, B, C and D and the production of biofilm in clinical isolates of MRSA. Fifty MRSA strains isolated from different types of clinical samples were studied. Detection of enterotoxins was carried out using the technique of reversed phase agglutination, while biofilm production was studied through two tests: Congo red agar and the microplate cell culture method. Enterotoxin production was observed in 9 strains (18%); enterotoxin D (64%) was the most prevalent, followed by B (27%) and A (9%). A significant association was shown between enterotoxin production capacity and the type of sample that came from the strain. Biofilm production was found in 30% and 98% of the strains using the Congo red Agar and microplate cell culture methods, respectively. A correlation of both trials was observed in only 15 strains (30%). It was shown that the microplate cell culture method is more effective for detecting biofilm production in S. aureus strains. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic |
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Directory of Open Access Journals: DOAJ Articles |
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English Spanish |
topic |
S. Aureus Resistente a Meticilina Enteroto Xinas Biofilm Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
S. Aureus Resistente a Meticilina Enteroto Xinas Biofilm Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Yeiny Ávila R. Messaria Ginestre P. Kutchynskaya Valero L. Maribel Castellano G. Sonia Romero A. Alfredo López Gresleida Rincón V. Lisette Sandrea T. Enterotoxin and Biofilm Production in Clinical Isolates of Methicillin-Resistant Staphylococcus aureus |
topic_facet |
S. Aureus Resistente a Meticilina Enteroto Xinas Biofilm Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
S. aureus has become a public health problem, due to the difficulty of treating infections caused by methicillin-resistant S. aureus (MRSA). The purpose of this research was to determine the production of enterotoxins A, B, C and D and the production of biofilm in clinical isolates of MRSA. Fifty MRSA strains isolated from different types of clinical samples were studied. Detection of enterotoxins was carried out using the technique of reversed phase agglutination, while biofilm production was studied through two tests: Congo red agar and the microplate cell culture method. Enterotoxin production was observed in 9 strains (18%); enterotoxin D (64%) was the most prevalent, followed by B (27%) and A (9%). A significant association was shown between enterotoxin production capacity and the type of sample that came from the strain. Biofilm production was found in 30% and 98% of the strains using the Congo red Agar and microplate cell culture methods, respectively. A correlation of both trials was observed in only 15 strains (30%). It was shown that the microplate cell culture method is more effective for detecting biofilm production in S. aureus strains. |
format |
Article in Journal/Newspaper |
author |
Yeiny Ávila R. Messaria Ginestre P. Kutchynskaya Valero L. Maribel Castellano G. Sonia Romero A. Alfredo López Gresleida Rincón V. Lisette Sandrea T. |
author_facet |
Yeiny Ávila R. Messaria Ginestre P. Kutchynskaya Valero L. Maribel Castellano G. Sonia Romero A. Alfredo López Gresleida Rincón V. Lisette Sandrea T. |
author_sort |
Yeiny Ávila R. |
title |
Enterotoxin and Biofilm Production in Clinical Isolates of Methicillin-Resistant Staphylococcus aureus |
title_short |
Enterotoxin and Biofilm Production in Clinical Isolates of Methicillin-Resistant Staphylococcus aureus |
title_full |
Enterotoxin and Biofilm Production in Clinical Isolates of Methicillin-Resistant Staphylococcus aureus |
title_fullStr |
Enterotoxin and Biofilm Production in Clinical Isolates of Methicillin-Resistant Staphylococcus aureus |
title_full_unstemmed |
Enterotoxin and Biofilm Production in Clinical Isolates of Methicillin-Resistant Staphylococcus aureus |
title_sort |
enterotoxin and biofilm production in clinical isolates of methicillin-resistant staphylococcus aureus |
publisher |
Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales |
publishDate |
2014 |
url |
https://doaj.org/article/eb78217179164b63ba4bae95c9b270f4 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Kasmera, Vol 42, Iss 2, Pp 105-115 (2014) |
op_relation |
https://produccioncientificaluz.org/index.php/kasmera/article/view/19498 https://doaj.org/toc/0075-5222 https://doaj.org/toc/2477-9628 0075-5222 2477-9628 https://doaj.org/article/eb78217179164b63ba4bae95c9b270f4 |
_version_ |
1778521441161969664 |