Neurocryptococcosis: diagnosis by PCR method Neurocriptococose: diagnóstico por PCR
Cryptococcus neoformans detection was optimized using PCR technique with the objective of application in the clinical laboratory diagnosis. The amplification area was ITS and 5,6S which encodes the ribosomal RNA (rRNA). A total of 72 cerebrospinal fluid (CSF) samples were used, obtained from cases w...
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Universidade de São Paulo (USP)
2004
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ftdoajarticles:oai:doaj.org/article:eb7384377c5b49298327d0e19a47f27e 2024-09-09T19:27:29+00:00 Neurocryptococcosis: diagnosis by PCR method Neurocriptococose: diagnóstico por PCR Regina Célia Paschoal Mário Hiroyuki Hirata Rosário Crespo Hirata Márcia de Souza Carvalho Melhem Amanda Latercia Tranches Dias Claudete Rodrigues Paula 2004-08-01T00:00:00Z https://doi.org/10.1590/S0036-46652004000400006 https://doaj.org/article/eb7384377c5b49298327d0e19a47f27e EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652004000400006 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46652004000400006 0036-4665 1678-9946 https://doaj.org/article/eb7384377c5b49298327d0e19a47f27e Revista do Instituto de Medicina Tropical de São Paulo, Vol 46, Iss 4, Pp 203-207 (2004) Cryptococcus neoformans PCR Diagnostic methods AIDS Cryptococcosis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2004 ftdoajarticles https://doi.org/10.1590/S0036-46652004000400006 2024-08-05T17:49:30Z Cryptococcus neoformans detection was optimized using PCR technique with the objective of application in the clinical laboratory diagnosis. The amplification area was ITS and 5,6S which encodes the ribosomal RNA (rRNA). A total of 72 cerebrospinal fluid (CSF) samples were used, obtained from cases with and without AIDS. The patients had cryptococcal meningitis (n = 56) and meningitis caused by other agents (n = 16). The results demonstrated that PCR test had the highest sensitivity rates, superior to culture (85.7%) and to India ink test (76.8%). PCR was found to be sensitive in detecting 1 cell/mL and highly specific since it did not amplify other fungal DNA. The comparative analysis of the methods showed that PCR is more sensitive and specific and is applicable as an important laboratorial resource for neurocryptococcosis diagnosis. A detecção de Cryptococcus neoformans em líquor foi otimizada pela técnica de PCR. A amplificação foi realizada nas áreas ITS e 5,6S do RNA ribossomal (rRNA). Foram estudados 72 líquors obtidos de casos de pacientes com e sem AIDS. Os pacientes eram portadores de meningite criptocócica (n = 56) e meningite ocasionada por outros agentes (n = 16). Os resultados demonstraram que a técnica tem alta sensibilidade, superior a cultura (85,7%) e ao teste da tinta da china (76,8%). A técnica de PCR pode detectar 1 célula/mL de líquor e é altamente específica. A análise comparativa dos três métodos, tinta da china, cultura e PCR, demonstrou que o último é muito mais sensível e específico, podendo ser aplicável como importante recurso laboratorial no diagnóstico da neurocriptococose. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Alta Arctic Revista do Instituto de Medicina Tropical de São Paulo 46 4 203 207 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Cryptococcus neoformans PCR Diagnostic methods AIDS Cryptococcosis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Cryptococcus neoformans PCR Diagnostic methods AIDS Cryptococcosis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Regina Célia Paschoal Mário Hiroyuki Hirata Rosário Crespo Hirata Márcia de Souza Carvalho Melhem Amanda Latercia Tranches Dias Claudete Rodrigues Paula Neurocryptococcosis: diagnosis by PCR method Neurocriptococose: diagnóstico por PCR |
topic_facet |
Cryptococcus neoformans PCR Diagnostic methods AIDS Cryptococcosis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Cryptococcus neoformans detection was optimized using PCR technique with the objective of application in the clinical laboratory diagnosis. The amplification area was ITS and 5,6S which encodes the ribosomal RNA (rRNA). A total of 72 cerebrospinal fluid (CSF) samples were used, obtained from cases with and without AIDS. The patients had cryptococcal meningitis (n = 56) and meningitis caused by other agents (n = 16). The results demonstrated that PCR test had the highest sensitivity rates, superior to culture (85.7%) and to India ink test (76.8%). PCR was found to be sensitive in detecting 1 cell/mL and highly specific since it did not amplify other fungal DNA. The comparative analysis of the methods showed that PCR is more sensitive and specific and is applicable as an important laboratorial resource for neurocryptococcosis diagnosis. A detecção de Cryptococcus neoformans em líquor foi otimizada pela técnica de PCR. A amplificação foi realizada nas áreas ITS e 5,6S do RNA ribossomal (rRNA). Foram estudados 72 líquors obtidos de casos de pacientes com e sem AIDS. Os pacientes eram portadores de meningite criptocócica (n = 56) e meningite ocasionada por outros agentes (n = 16). Os resultados demonstraram que a técnica tem alta sensibilidade, superior a cultura (85,7%) e ao teste da tinta da china (76,8%). A técnica de PCR pode detectar 1 célula/mL de líquor e é altamente específica. A análise comparativa dos três métodos, tinta da china, cultura e PCR, demonstrou que o último é muito mais sensível e específico, podendo ser aplicável como importante recurso laboratorial no diagnóstico da neurocriptococose. |
format |
Article in Journal/Newspaper |
author |
Regina Célia Paschoal Mário Hiroyuki Hirata Rosário Crespo Hirata Márcia de Souza Carvalho Melhem Amanda Latercia Tranches Dias Claudete Rodrigues Paula |
author_facet |
Regina Célia Paschoal Mário Hiroyuki Hirata Rosário Crespo Hirata Márcia de Souza Carvalho Melhem Amanda Latercia Tranches Dias Claudete Rodrigues Paula |
author_sort |
Regina Célia Paschoal |
title |
Neurocryptococcosis: diagnosis by PCR method Neurocriptococose: diagnóstico por PCR |
title_short |
Neurocryptococcosis: diagnosis by PCR method Neurocriptococose: diagnóstico por PCR |
title_full |
Neurocryptococcosis: diagnosis by PCR method Neurocriptococose: diagnóstico por PCR |
title_fullStr |
Neurocryptococcosis: diagnosis by PCR method Neurocriptococose: diagnóstico por PCR |
title_full_unstemmed |
Neurocryptococcosis: diagnosis by PCR method Neurocriptococose: diagnóstico por PCR |
title_sort |
neurocryptococcosis: diagnosis by pcr method neurocriptococose: diagnóstico por pcr |
publisher |
Universidade de São Paulo (USP) |
publishDate |
2004 |
url |
https://doi.org/10.1590/S0036-46652004000400006 https://doaj.org/article/eb7384377c5b49298327d0e19a47f27e |
geographic |
Alta Arctic |
geographic_facet |
Alta Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 46, Iss 4, Pp 203-207 (2004) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652004000400006 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46652004000400006 0036-4665 1678-9946 https://doaj.org/article/eb7384377c5b49298327d0e19a47f27e |
op_doi |
https://doi.org/10.1590/S0036-46652004000400006 |
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Revista do Instituto de Medicina Tropical de São Paulo |
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46 |
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4 |
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203 |
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207 |
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