Evaluation of the geographical utility of Eastern Russell's viper (Daboia siamensis) antivenom from Thailand and an assessment of its protective effects against venom-induced nephrotoxicity.
Background Daboia siamensis (Eastern Russell's viper) is a medically important snake species found widely distributed across Southeast Asia. Envenomings by this species can result in systemic coagulopathy, local tissue injury and/or renal failure. While administration of specific antivenom is a...
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ftdoajarticles:oai:doaj.org/article:eb6bfd63d36741d3b74b054c9c135be3 2023-05-15T15:15:27+02:00 Evaluation of the geographical utility of Eastern Russell's viper (Daboia siamensis) antivenom from Thailand and an assessment of its protective effects against venom-induced nephrotoxicity. Janeyuth Chaisakul Jaffer Alsolaiss Mongkon Charoenpitakchai Kulachet Wiwatwarayos Nattapon Sookprasert Robert A Harrison Narongsak Chaiyabutr Lawan Chanhome Choo Hock Tan Nicholas R Casewell 2019-10-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0007338 https://doaj.org/article/eb6bfd63d36741d3b74b054c9c135be3 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0007338 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007338 https://doaj.org/article/eb6bfd63d36741d3b74b054c9c135be3 PLoS Neglected Tropical Diseases, Vol 13, Iss 10, p e0007338 (2019) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2019 ftdoajarticles https://doi.org/10.1371/journal.pntd.0007338 2022-12-31T16:26:56Z Background Daboia siamensis (Eastern Russell's viper) is a medically important snake species found widely distributed across Southeast Asia. Envenomings by this species can result in systemic coagulopathy, local tissue injury and/or renal failure. While administration of specific antivenom is an effective treatment for Russell's viper envenomings, the availability of, and access to, geographically-appropriate antivenom remains problematic in many rural areas. In this study, we determined the binding and neutralizing capability of antivenoms manufactured by the Thai Red Cross in Thailand against D. siamensis venoms from four geographical locales: Myanmar, Taiwan, China and Thailand. Methodology/principle findings The D. siamensis monovalent antivenom displayed extensive recognition and binding to proteins found in D. siamensis venom, irrespective of the geographical origin of those venoms. Similar immunological characteristics were observed with the Hemato Polyvalent antivenom, which also uses D. siamensis venom as an immunogen, but binding levels were dramatically reduced when using comparator monovalent antivenoms manufactured against different snake species. A similar pattern was observed when investigating neutralization of coagulopathy, with the procoagulant action of all four geographical venom variants neutralized by both the D. siamensis monovalent and the Hemato Polyvalent antivenoms, while the comparator monovalent antivenoms were ineffective. These in vitro findings translated into therapeutic efficacy in vivo, as the D. siamensis monovalent antivenom was found to effectively protect against the lethal effects of all four geographical venom variants preclinically. Assessments of in vivo nephrotoxicity revealed that D. siamensis venom (700 μg/kg) significantly increased plasma creatinine and blood urea nitrogen levels in anaesthetised rats. The intravenous administration of D. siamensis monovalent antivenom at three times higher than the recommended scaled therapeutic dose, prior to and 1 h after the ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 13 10 e0007338 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Janeyuth Chaisakul Jaffer Alsolaiss Mongkon Charoenpitakchai Kulachet Wiwatwarayos Nattapon Sookprasert Robert A Harrison Narongsak Chaiyabutr Lawan Chanhome Choo Hock Tan Nicholas R Casewell Evaluation of the geographical utility of Eastern Russell's viper (Daboia siamensis) antivenom from Thailand and an assessment of its protective effects against venom-induced nephrotoxicity. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Background Daboia siamensis (Eastern Russell's viper) is a medically important snake species found widely distributed across Southeast Asia. Envenomings by this species can result in systemic coagulopathy, local tissue injury and/or renal failure. While administration of specific antivenom is an effective treatment for Russell's viper envenomings, the availability of, and access to, geographically-appropriate antivenom remains problematic in many rural areas. In this study, we determined the binding and neutralizing capability of antivenoms manufactured by the Thai Red Cross in Thailand against D. siamensis venoms from four geographical locales: Myanmar, Taiwan, China and Thailand. Methodology/principle findings The D. siamensis monovalent antivenom displayed extensive recognition and binding to proteins found in D. siamensis venom, irrespective of the geographical origin of those venoms. Similar immunological characteristics were observed with the Hemato Polyvalent antivenom, which also uses D. siamensis venom as an immunogen, but binding levels were dramatically reduced when using comparator monovalent antivenoms manufactured against different snake species. A similar pattern was observed when investigating neutralization of coagulopathy, with the procoagulant action of all four geographical venom variants neutralized by both the D. siamensis monovalent and the Hemato Polyvalent antivenoms, while the comparator monovalent antivenoms were ineffective. These in vitro findings translated into therapeutic efficacy in vivo, as the D. siamensis monovalent antivenom was found to effectively protect against the lethal effects of all four geographical venom variants preclinically. Assessments of in vivo nephrotoxicity revealed that D. siamensis venom (700 μg/kg) significantly increased plasma creatinine and blood urea nitrogen levels in anaesthetised rats. The intravenous administration of D. siamensis monovalent antivenom at three times higher than the recommended scaled therapeutic dose, prior to and 1 h after the ... |
format |
Article in Journal/Newspaper |
author |
Janeyuth Chaisakul Jaffer Alsolaiss Mongkon Charoenpitakchai Kulachet Wiwatwarayos Nattapon Sookprasert Robert A Harrison Narongsak Chaiyabutr Lawan Chanhome Choo Hock Tan Nicholas R Casewell |
author_facet |
Janeyuth Chaisakul Jaffer Alsolaiss Mongkon Charoenpitakchai Kulachet Wiwatwarayos Nattapon Sookprasert Robert A Harrison Narongsak Chaiyabutr Lawan Chanhome Choo Hock Tan Nicholas R Casewell |
author_sort |
Janeyuth Chaisakul |
title |
Evaluation of the geographical utility of Eastern Russell's viper (Daboia siamensis) antivenom from Thailand and an assessment of its protective effects against venom-induced nephrotoxicity. |
title_short |
Evaluation of the geographical utility of Eastern Russell's viper (Daboia siamensis) antivenom from Thailand and an assessment of its protective effects against venom-induced nephrotoxicity. |
title_full |
Evaluation of the geographical utility of Eastern Russell's viper (Daboia siamensis) antivenom from Thailand and an assessment of its protective effects against venom-induced nephrotoxicity. |
title_fullStr |
Evaluation of the geographical utility of Eastern Russell's viper (Daboia siamensis) antivenom from Thailand and an assessment of its protective effects against venom-induced nephrotoxicity. |
title_full_unstemmed |
Evaluation of the geographical utility of Eastern Russell's viper (Daboia siamensis) antivenom from Thailand and an assessment of its protective effects against venom-induced nephrotoxicity. |
title_sort |
evaluation of the geographical utility of eastern russell's viper (daboia siamensis) antivenom from thailand and an assessment of its protective effects against venom-induced nephrotoxicity. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2019 |
url |
https://doi.org/10.1371/journal.pntd.0007338 https://doaj.org/article/eb6bfd63d36741d3b74b054c9c135be3 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 13, Iss 10, p e0007338 (2019) |
op_relation |
https://doi.org/10.1371/journal.pntd.0007338 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007338 https://doaj.org/article/eb6bfd63d36741d3b74b054c9c135be3 |
op_doi |
https://doi.org/10.1371/journal.pntd.0007338 |
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PLOS Neglected Tropical Diseases |
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13 |
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10 |
container_start_page |
e0007338 |
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