Composite beads of silica gel, alginate and poly(aspartic acid) for the immobilization of a lipase enzyme
Silica gel/alginate/poly(aspartic acid) composite beads were prepared for immobilization of lipase B enzyme from Candida antarctica (CaLB). CaLB was first adsorbed on functionalized mesoporous silica gel particles, which were then entrapped in the interpenetrating network of thiolated poly(aspartic...
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Budapest University of Technology
2019
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ftdoajarticles:oai:doaj.org/article:eb015ad0ea694330b3b47c14f79633fb 2023-05-15T13:45:41+02:00 Composite beads of silica gel, alginate and poly(aspartic acid) for the immobilization of a lipase enzyme E. Krisch D. Balogh-Weiser J. Klimko B. Gyarmati K. Laszlo L. Poppe A. Szilagyi 2019-06-01T00:00:00Z https://doi.org/10.3144/expresspolymlett.2019.43 https://doaj.org/article/eb015ad0ea694330b3b47c14f79633fb EN eng Budapest University of Technology http://www.expresspolymlett.com/letolt.php?file=EPL-0009532&mi=cd https://doaj.org/toc/1788-618X 1788-618X doi:10.3144/expresspolymlett.2019.43 https://doaj.org/article/eb015ad0ea694330b3b47c14f79633fb eXPRESS Polymer Letters, Vol 13, Iss 6, Pp 512-523 (2019) Polymer composites Biodegradable polymers Biocatalysis Enzyme Kinetic resolution Materials of engineering and construction. Mechanics of materials TA401-492 Chemical technology TP1-1185 article 2019 ftdoajarticles https://doi.org/10.3144/expresspolymlett.2019.43 2022-12-31T08:49:58Z Silica gel/alginate/poly(aspartic acid) composite beads were prepared for immobilization of lipase B enzyme from Candida antarctica (CaLB). CaLB was first adsorbed on functionalized mesoporous silica gel particles, which were then entrapped in the interpenetrating network of thiolated poly(aspartic acid) and alginate, cross-linked by zinc ions. Finally, the beads were chemically stabilized by poly(ethylene glycol) diglycidyl ether, a bisepoxide cross-linker. In this manner, spherical biocatalysts with a diameter of 3–4 mm were prepared and their biocatalytic performance was tested by kinetic resolution of racemic 1-phenylethanol. The activity of CaLB in the beads was comparable to that of CaLB physically adsorbed on silica gel particles. The composite beads were easy to recover after use and no loss of biocatalytic activity was observed even after five test reaction cycles. Furthermore, the CaLB in the composite beads showed sufficient thermal stability up to 90 °C, contrary to CaLB adsorbed only on silica gel particles. Article in Journal/Newspaper Antarc* Antarctica Directory of Open Access Journals: DOAJ Articles Express Polymer Letters 13 6 512 523 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Polymer composites Biodegradable polymers Biocatalysis Enzyme Kinetic resolution Materials of engineering and construction. Mechanics of materials TA401-492 Chemical technology TP1-1185 |
spellingShingle |
Polymer composites Biodegradable polymers Biocatalysis Enzyme Kinetic resolution Materials of engineering and construction. Mechanics of materials TA401-492 Chemical technology TP1-1185 E. Krisch D. Balogh-Weiser J. Klimko B. Gyarmati K. Laszlo L. Poppe A. Szilagyi Composite beads of silica gel, alginate and poly(aspartic acid) for the immobilization of a lipase enzyme |
topic_facet |
Polymer composites Biodegradable polymers Biocatalysis Enzyme Kinetic resolution Materials of engineering and construction. Mechanics of materials TA401-492 Chemical technology TP1-1185 |
description |
Silica gel/alginate/poly(aspartic acid) composite beads were prepared for immobilization of lipase B enzyme from Candida antarctica (CaLB). CaLB was first adsorbed on functionalized mesoporous silica gel particles, which were then entrapped in the interpenetrating network of thiolated poly(aspartic acid) and alginate, cross-linked by zinc ions. Finally, the beads were chemically stabilized by poly(ethylene glycol) diglycidyl ether, a bisepoxide cross-linker. In this manner, spherical biocatalysts with a diameter of 3–4 mm were prepared and their biocatalytic performance was tested by kinetic resolution of racemic 1-phenylethanol. The activity of CaLB in the beads was comparable to that of CaLB physically adsorbed on silica gel particles. The composite beads were easy to recover after use and no loss of biocatalytic activity was observed even after five test reaction cycles. Furthermore, the CaLB in the composite beads showed sufficient thermal stability up to 90 °C, contrary to CaLB adsorbed only on silica gel particles. |
format |
Article in Journal/Newspaper |
author |
E. Krisch D. Balogh-Weiser J. Klimko B. Gyarmati K. Laszlo L. Poppe A. Szilagyi |
author_facet |
E. Krisch D. Balogh-Weiser J. Klimko B. Gyarmati K. Laszlo L. Poppe A. Szilagyi |
author_sort |
E. Krisch |
title |
Composite beads of silica gel, alginate and poly(aspartic acid) for the immobilization of a lipase enzyme |
title_short |
Composite beads of silica gel, alginate and poly(aspartic acid) for the immobilization of a lipase enzyme |
title_full |
Composite beads of silica gel, alginate and poly(aspartic acid) for the immobilization of a lipase enzyme |
title_fullStr |
Composite beads of silica gel, alginate and poly(aspartic acid) for the immobilization of a lipase enzyme |
title_full_unstemmed |
Composite beads of silica gel, alginate and poly(aspartic acid) for the immobilization of a lipase enzyme |
title_sort |
composite beads of silica gel, alginate and poly(aspartic acid) for the immobilization of a lipase enzyme |
publisher |
Budapest University of Technology |
publishDate |
2019 |
url |
https://doi.org/10.3144/expresspolymlett.2019.43 https://doaj.org/article/eb015ad0ea694330b3b47c14f79633fb |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
eXPRESS Polymer Letters, Vol 13, Iss 6, Pp 512-523 (2019) |
op_relation |
http://www.expresspolymlett.com/letolt.php?file=EPL-0009532&mi=cd https://doaj.org/toc/1788-618X 1788-618X doi:10.3144/expresspolymlett.2019.43 https://doaj.org/article/eb015ad0ea694330b3b47c14f79633fb |
op_doi |
https://doi.org/10.3144/expresspolymlett.2019.43 |
container_title |
Express Polymer Letters |
container_volume |
13 |
container_issue |
6 |
container_start_page |
512 |
op_container_end_page |
523 |
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1766229745719050240 |