Amplicon-Metagenomic Analysis of Fungi from Antarctic Terrestrial Habitats
In cold environments such as polar regions, microorganisms play important ecological roles, and most of our knowledge about them comes from studies of cultivable microorganisms. Metagenomic technologies are powerful tools that can give a more comprehensive assessment of microbial communities, and th...
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ftdoajarticles:oai:doaj.org/article:eafac21749fb4ea89f9ab4bc68d0c645 2023-05-15T13:52:04+02:00 Amplicon-Metagenomic Analysis of Fungi from Antarctic Terrestrial Habitats Marcelo Baeza Salvador Barahona Jennifer Alcaíno Víctor Cifuentes 2017-11-01T00:00:00Z https://doi.org/10.3389/fmicb.2017.02235 https://doaj.org/article/eafac21749fb4ea89f9ab4bc68d0c645 EN eng Frontiers Media S.A. http://journal.frontiersin.org/article/10.3389/fmicb.2017.02235/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2017.02235 https://doaj.org/article/eafac21749fb4ea89f9ab4bc68d0c645 Frontiers in Microbiology, Vol 8 (2017) Antarctic microeukaryotes amplicon-metagenome Antarctic fungi Antarctic diatoms cold-terrestrial microeukaryotes Microbiology QR1-502 article 2017 ftdoajarticles https://doi.org/10.3389/fmicb.2017.02235 2022-12-31T13:07:27Z In cold environments such as polar regions, microorganisms play important ecological roles, and most of our knowledge about them comes from studies of cultivable microorganisms. Metagenomic technologies are powerful tools that can give a more comprehensive assessment of microbial communities, and the amplification of rDNA followed by next-generation sequencing has given good results in studies aimed particularly at environmental microorganisms. Culture-independent studies of microbiota in terrestrial habitats of Antarctica, which is considered the driest, coldest climate on Earth, are increasing and indicate that micro-diversity is much higher than previously thought. In this work, the microbial diversity of terrestrial habitats including eight islands of the South Shetland Archipelago, two islands on the Antarctic Peninsula and Union Glacier, was studied by amplicon-metagenome analysis. Molecular analysis of the studied localities clustered together the islands of the South Shetland Archipelago, except Greenwich Island, and separated them from the Litchfield and Lagotellerie islands and Union Glacier, which is in agreement with the latitudinal difference among them. Among fungi, 87 genera and 123 species were found, of which species belonging to 37 fungal genera not previously cultivated from Antarctica were detected. Phylogenetic analysis, including the closest BLAST-hit sequences, clustered fungi in 11 classes being the most represented Lecanoromycetes and Eurotiomycetes. Article in Journal/Newspaper Antarc* Antarctic Antarctic Peninsula Antarctica Greenwich Island Union Glacier Directory of Open Access Journals: DOAJ Articles Antarctic Antarctic Peninsula Greenwich Greenwich Island ENVELOPE(-59.783,-59.783,-62.517,-62.517) Lagotellerie ENVELOPE(-67.402,-67.402,-67.886,-67.886) Litchfield ENVELOPE(-64.100,-64.100,-64.783,-64.783) The Antarctic Union Glacier ENVELOPE(-82.500,-82.500,-79.750,-79.750) Frontiers in Microbiology 8 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Antarctic microeukaryotes amplicon-metagenome Antarctic fungi Antarctic diatoms cold-terrestrial microeukaryotes Microbiology QR1-502 |
spellingShingle |
Antarctic microeukaryotes amplicon-metagenome Antarctic fungi Antarctic diatoms cold-terrestrial microeukaryotes Microbiology QR1-502 Marcelo Baeza Salvador Barahona Jennifer Alcaíno Víctor Cifuentes Amplicon-Metagenomic Analysis of Fungi from Antarctic Terrestrial Habitats |
topic_facet |
Antarctic microeukaryotes amplicon-metagenome Antarctic fungi Antarctic diatoms cold-terrestrial microeukaryotes Microbiology QR1-502 |
description |
In cold environments such as polar regions, microorganisms play important ecological roles, and most of our knowledge about them comes from studies of cultivable microorganisms. Metagenomic technologies are powerful tools that can give a more comprehensive assessment of microbial communities, and the amplification of rDNA followed by next-generation sequencing has given good results in studies aimed particularly at environmental microorganisms. Culture-independent studies of microbiota in terrestrial habitats of Antarctica, which is considered the driest, coldest climate on Earth, are increasing and indicate that micro-diversity is much higher than previously thought. In this work, the microbial diversity of terrestrial habitats including eight islands of the South Shetland Archipelago, two islands on the Antarctic Peninsula and Union Glacier, was studied by amplicon-metagenome analysis. Molecular analysis of the studied localities clustered together the islands of the South Shetland Archipelago, except Greenwich Island, and separated them from the Litchfield and Lagotellerie islands and Union Glacier, which is in agreement with the latitudinal difference among them. Among fungi, 87 genera and 123 species were found, of which species belonging to 37 fungal genera not previously cultivated from Antarctica were detected. Phylogenetic analysis, including the closest BLAST-hit sequences, clustered fungi in 11 classes being the most represented Lecanoromycetes and Eurotiomycetes. |
format |
Article in Journal/Newspaper |
author |
Marcelo Baeza Salvador Barahona Jennifer Alcaíno Víctor Cifuentes |
author_facet |
Marcelo Baeza Salvador Barahona Jennifer Alcaíno Víctor Cifuentes |
author_sort |
Marcelo Baeza |
title |
Amplicon-Metagenomic Analysis of Fungi from Antarctic Terrestrial Habitats |
title_short |
Amplicon-Metagenomic Analysis of Fungi from Antarctic Terrestrial Habitats |
title_full |
Amplicon-Metagenomic Analysis of Fungi from Antarctic Terrestrial Habitats |
title_fullStr |
Amplicon-Metagenomic Analysis of Fungi from Antarctic Terrestrial Habitats |
title_full_unstemmed |
Amplicon-Metagenomic Analysis of Fungi from Antarctic Terrestrial Habitats |
title_sort |
amplicon-metagenomic analysis of fungi from antarctic terrestrial habitats |
publisher |
Frontiers Media S.A. |
publishDate |
2017 |
url |
https://doi.org/10.3389/fmicb.2017.02235 https://doaj.org/article/eafac21749fb4ea89f9ab4bc68d0c645 |
long_lat |
ENVELOPE(-59.783,-59.783,-62.517,-62.517) ENVELOPE(-67.402,-67.402,-67.886,-67.886) ENVELOPE(-64.100,-64.100,-64.783,-64.783) ENVELOPE(-82.500,-82.500,-79.750,-79.750) |
geographic |
Antarctic Antarctic Peninsula Greenwich Greenwich Island Lagotellerie Litchfield The Antarctic Union Glacier |
geographic_facet |
Antarctic Antarctic Peninsula Greenwich Greenwich Island Lagotellerie Litchfield The Antarctic Union Glacier |
genre |
Antarc* Antarctic Antarctic Peninsula Antarctica Greenwich Island Union Glacier |
genre_facet |
Antarc* Antarctic Antarctic Peninsula Antarctica Greenwich Island Union Glacier |
op_source |
Frontiers in Microbiology, Vol 8 (2017) |
op_relation |
http://journal.frontiersin.org/article/10.3389/fmicb.2017.02235/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2017.02235 https://doaj.org/article/eafac21749fb4ea89f9ab4bc68d0c645 |
op_doi |
https://doi.org/10.3389/fmicb.2017.02235 |
container_title |
Frontiers in Microbiology |
container_volume |
8 |
_version_ |
1766256306030641152 |