Comparative analysis of the susceptibility of Aedes aegypti and Japanese Aedes albopictus to all dengue virus serotypes
Abstract Background Dengue fever, caused by the dengue virus (DENV), is the most common viral infection transmitted by Aedes mosquitoes (mainly Ae. aegypti and Ae. albopictus) worldwide. Aedes aegypti is not currently established in Japan, and Ae. albopictus is the primary vector mosquito for DENV i...
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ftdoajarticles:oai:doaj.org/article:eae820eb2140407db7d88189ebb173a2 2023-12-10T09:46:24+01:00 Comparative analysis of the susceptibility of Aedes aegypti and Japanese Aedes albopictus to all dengue virus serotypes Daisuke Kobayashi Izumi Kai Astri Nur Faizah Meng Ling Moi Shigeru Tajima Tomohiko Takasaki Toshinori Sasaki Haruhiko Isawa 2023-11-01T00:00:00Z https://doi.org/10.1186/s41182-023-00553-5 https://doaj.org/article/eae820eb2140407db7d88189ebb173a2 EN eng BMC https://doi.org/10.1186/s41182-023-00553-5 https://doaj.org/toc/1349-4147 doi:10.1186/s41182-023-00553-5 1349-4147 https://doaj.org/article/eae820eb2140407db7d88189ebb173a2 Tropical Medicine and Health, Vol 51, Iss 1, Pp 1-9 (2023) Dengue DENV DENV-1 Mosquito Aedes Aedes albopictus Arctic medicine. Tropical medicine RC955-962 article 2023 ftdoajarticles https://doi.org/10.1186/s41182-023-00553-5 2023-11-12T01:40:37Z Abstract Background Dengue fever, caused by the dengue virus (DENV), is the most common viral infection transmitted by Aedes mosquitoes (mainly Ae. aegypti and Ae. albopictus) worldwide. Aedes aegypti is not currently established in Japan, and Ae. albopictus is the primary vector mosquito for DENV in the country, but knowledge of its viral susceptibility is limited. Therefore, we aimed to clarify the status of DENV susceptibility by comparing the infection and dissemination dynamics of Japanese Ae. albopictus to all known DENV serotypes with those of Ae. aegypti. Methods After propagation of each DENV serotype in Vero cells, the culture supernatants were mixed with defibrinated rabbit blood and adenosine triphosphate, and the mixture was artificially blood-sucked by two colonies of Ae. albopictus from Japan and one colony of Ae. aegypti from a dengue-endemic country (Vietnam). After 14 days of sucking, the mosquito body was divided into two parts (thorax/abdomen and head/wings/legs) and total RNA was extracted from each sample. DENV RNA was detected in these extracted RNA samples using a quantitative RT-PCR method specific for each DENV serotype, and infection and dissemination rates were analyzed. Results The Japanese Ae. albopictus colonies were susceptible to all DENV serotypes. Its infection and dissemination rates were significantly lower than those of Ae. aegypti. However, the number of DENV RNA copies in Ae. albopictus was almost not significantly different from that in Ae. aegypti. Furthermore, Japanese Ae. albopictus differed widely in their susceptibility to each DENV serotype. Conclusions In Japanese Ae. albopictus, once DENV overcame the midgut infection barrier, the efficiency of subsequent propagation and dissemination of the virus in the mosquito body was comparable to that of Ae. aegypti. Based on the results of this study and previous dengue outbreak trends, Ae. albopictus is predicted to be highly compatible with DENV-1, suggesting that this serotype poses a high risk for future epidemics in ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Tropical Medicine and Health 51 1 |
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Directory of Open Access Journals: DOAJ Articles |
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Dengue DENV DENV-1 Mosquito Aedes Aedes albopictus Arctic medicine. Tropical medicine RC955-962 |
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Dengue DENV DENV-1 Mosquito Aedes Aedes albopictus Arctic medicine. Tropical medicine RC955-962 Daisuke Kobayashi Izumi Kai Astri Nur Faizah Meng Ling Moi Shigeru Tajima Tomohiko Takasaki Toshinori Sasaki Haruhiko Isawa Comparative analysis of the susceptibility of Aedes aegypti and Japanese Aedes albopictus to all dengue virus serotypes |
topic_facet |
Dengue DENV DENV-1 Mosquito Aedes Aedes albopictus Arctic medicine. Tropical medicine RC955-962 |
description |
Abstract Background Dengue fever, caused by the dengue virus (DENV), is the most common viral infection transmitted by Aedes mosquitoes (mainly Ae. aegypti and Ae. albopictus) worldwide. Aedes aegypti is not currently established in Japan, and Ae. albopictus is the primary vector mosquito for DENV in the country, but knowledge of its viral susceptibility is limited. Therefore, we aimed to clarify the status of DENV susceptibility by comparing the infection and dissemination dynamics of Japanese Ae. albopictus to all known DENV serotypes with those of Ae. aegypti. Methods After propagation of each DENV serotype in Vero cells, the culture supernatants were mixed with defibrinated rabbit blood and adenosine triphosphate, and the mixture was artificially blood-sucked by two colonies of Ae. albopictus from Japan and one colony of Ae. aegypti from a dengue-endemic country (Vietnam). After 14 days of sucking, the mosquito body was divided into two parts (thorax/abdomen and head/wings/legs) and total RNA was extracted from each sample. DENV RNA was detected in these extracted RNA samples using a quantitative RT-PCR method specific for each DENV serotype, and infection and dissemination rates were analyzed. Results The Japanese Ae. albopictus colonies were susceptible to all DENV serotypes. Its infection and dissemination rates were significantly lower than those of Ae. aegypti. However, the number of DENV RNA copies in Ae. albopictus was almost not significantly different from that in Ae. aegypti. Furthermore, Japanese Ae. albopictus differed widely in their susceptibility to each DENV serotype. Conclusions In Japanese Ae. albopictus, once DENV overcame the midgut infection barrier, the efficiency of subsequent propagation and dissemination of the virus in the mosquito body was comparable to that of Ae. aegypti. Based on the results of this study and previous dengue outbreak trends, Ae. albopictus is predicted to be highly compatible with DENV-1, suggesting that this serotype poses a high risk for future epidemics in ... |
format |
Article in Journal/Newspaper |
author |
Daisuke Kobayashi Izumi Kai Astri Nur Faizah Meng Ling Moi Shigeru Tajima Tomohiko Takasaki Toshinori Sasaki Haruhiko Isawa |
author_facet |
Daisuke Kobayashi Izumi Kai Astri Nur Faizah Meng Ling Moi Shigeru Tajima Tomohiko Takasaki Toshinori Sasaki Haruhiko Isawa |
author_sort |
Daisuke Kobayashi |
title |
Comparative analysis of the susceptibility of Aedes aegypti and Japanese Aedes albopictus to all dengue virus serotypes |
title_short |
Comparative analysis of the susceptibility of Aedes aegypti and Japanese Aedes albopictus to all dengue virus serotypes |
title_full |
Comparative analysis of the susceptibility of Aedes aegypti and Japanese Aedes albopictus to all dengue virus serotypes |
title_fullStr |
Comparative analysis of the susceptibility of Aedes aegypti and Japanese Aedes albopictus to all dengue virus serotypes |
title_full_unstemmed |
Comparative analysis of the susceptibility of Aedes aegypti and Japanese Aedes albopictus to all dengue virus serotypes |
title_sort |
comparative analysis of the susceptibility of aedes aegypti and japanese aedes albopictus to all dengue virus serotypes |
publisher |
BMC |
publishDate |
2023 |
url |
https://doi.org/10.1186/s41182-023-00553-5 https://doaj.org/article/eae820eb2140407db7d88189ebb173a2 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Tropical Medicine and Health, Vol 51, Iss 1, Pp 1-9 (2023) |
op_relation |
https://doi.org/10.1186/s41182-023-00553-5 https://doaj.org/toc/1349-4147 doi:10.1186/s41182-023-00553-5 1349-4147 https://doaj.org/article/eae820eb2140407db7d88189ebb173a2 |
op_doi |
https://doi.org/10.1186/s41182-023-00553-5 |
container_title |
Tropical Medicine and Health |
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51 |
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1 |
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1784889771384897536 |