Genetic variation of TLR-4 , TLR-9 and TIRAP genes in Iranian malaria patients
Abstract Background Toll-like receptors (TLRs) recognize pathogen-associated molecular patterns and their activation leads to the induction of effector genes involving inflammatory cytokines that may have contribute to controlling parasite growth and disease pathogenesis. The current immunogenetic s...
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ftdoajarticles:oai:doaj.org/article:eacadae29e9b4a1786b09638abaeb77e 2023-05-15T15:13:09+02:00 Genetic variation of TLR-4 , TLR-9 and TIRAP genes in Iranian malaria patients Mehrizi Akram A Pirahmadi Sakineh Zakeri Sedigheh Djadid Navid D 2011-04-01T00:00:00Z https://doi.org/10.1186/1475-2875-10-77 https://doaj.org/article/eacadae29e9b4a1786b09638abaeb77e EN eng BMC http://www.malariajournal.com/content/10/1/77 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-10-77 1475-2875 https://doaj.org/article/eacadae29e9b4a1786b09638abaeb77e Malaria Journal, Vol 10, Iss 1, p 77 (2011) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2011 ftdoajarticles https://doi.org/10.1186/1475-2875-10-77 2022-12-31T00:28:39Z Abstract Background Toll-like receptors (TLRs) recognize pathogen-associated molecular patterns and their activation leads to the induction of effector genes involving inflammatory cytokines that may have contribute to controlling parasite growth and disease pathogenesis. The current immunogenetic study was designed to analyse the key components of innate immunity, TLRs and TIRAP (Toll-interleukin-1 receptor domain-containing adaptor protein), also known as MAL (MYD88 adaptor-like), in Iranian patients with mild malaria. Methods The tlr - 4 (D299G and T399I), tlr-9 (T-1486C and T-1237C) and tirap (S180L) genes were assessed in 640 Baluchi individuals (320 Plasmodium falciparum -infected and 320 non-infected, median age of 28 years) from malaria-endemic regions using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) methods. Results Common tlr-4 SNPs and promoter SNPs of tlr-9 were distributed among P. falciparum -infected and non-infected groups ( P > 0.05) that showed no association of these variants with mild clinical manifestation. The comparison of the tirap S180L genotypes between patients with mild malaria and those healthy individuals showed that the frequency of heterozygosity was significantly higher in infected than non-infected individuals (33.8 vs. 25.6; OR, 1.479; 95% CI, 1.051-2.081; P = 0.024). The result also revealed a significant association of tirap S180L ( P < 0.05) with development of mild malaria, which is common in Baluchi populations, who are living in malaria hypoendemic region of Iran but not in African populations (0%-6%). Conclusion These data point towards the need for addressing the exact role of TLRs in contributing to human genetic factors in malaria susceptibility/resistance/severity within different malaria settings in the world. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 10 1 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Mehrizi Akram A Pirahmadi Sakineh Zakeri Sedigheh Djadid Navid D Genetic variation of TLR-4 , TLR-9 and TIRAP genes in Iranian malaria patients |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Toll-like receptors (TLRs) recognize pathogen-associated molecular patterns and their activation leads to the induction of effector genes involving inflammatory cytokines that may have contribute to controlling parasite growth and disease pathogenesis. The current immunogenetic study was designed to analyse the key components of innate immunity, TLRs and TIRAP (Toll-interleukin-1 receptor domain-containing adaptor protein), also known as MAL (MYD88 adaptor-like), in Iranian patients with mild malaria. Methods The tlr - 4 (D299G and T399I), tlr-9 (T-1486C and T-1237C) and tirap (S180L) genes were assessed in 640 Baluchi individuals (320 Plasmodium falciparum -infected and 320 non-infected, median age of 28 years) from malaria-endemic regions using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) methods. Results Common tlr-4 SNPs and promoter SNPs of tlr-9 were distributed among P. falciparum -infected and non-infected groups ( P > 0.05) that showed no association of these variants with mild clinical manifestation. The comparison of the tirap S180L genotypes between patients with mild malaria and those healthy individuals showed that the frequency of heterozygosity was significantly higher in infected than non-infected individuals (33.8 vs. 25.6; OR, 1.479; 95% CI, 1.051-2.081; P = 0.024). The result also revealed a significant association of tirap S180L ( P < 0.05) with development of mild malaria, which is common in Baluchi populations, who are living in malaria hypoendemic region of Iran but not in African populations (0%-6%). Conclusion These data point towards the need for addressing the exact role of TLRs in contributing to human genetic factors in malaria susceptibility/resistance/severity within different malaria settings in the world. |
format |
Article in Journal/Newspaper |
author |
Mehrizi Akram A Pirahmadi Sakineh Zakeri Sedigheh Djadid Navid D |
author_facet |
Mehrizi Akram A Pirahmadi Sakineh Zakeri Sedigheh Djadid Navid D |
author_sort |
Mehrizi Akram A |
title |
Genetic variation of TLR-4 , TLR-9 and TIRAP genes in Iranian malaria patients |
title_short |
Genetic variation of TLR-4 , TLR-9 and TIRAP genes in Iranian malaria patients |
title_full |
Genetic variation of TLR-4 , TLR-9 and TIRAP genes in Iranian malaria patients |
title_fullStr |
Genetic variation of TLR-4 , TLR-9 and TIRAP genes in Iranian malaria patients |
title_full_unstemmed |
Genetic variation of TLR-4 , TLR-9 and TIRAP genes in Iranian malaria patients |
title_sort |
genetic variation of tlr-4 , tlr-9 and tirap genes in iranian malaria patients |
publisher |
BMC |
publishDate |
2011 |
url |
https://doi.org/10.1186/1475-2875-10-77 https://doaj.org/article/eacadae29e9b4a1786b09638abaeb77e |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 10, Iss 1, p 77 (2011) |
op_relation |
http://www.malariajournal.com/content/10/1/77 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-10-77 1475-2875 https://doaj.org/article/eacadae29e9b4a1786b09638abaeb77e |
op_doi |
https://doi.org/10.1186/1475-2875-10-77 |
container_title |
Malaria Journal |
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10 |
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1 |
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1766343742152769536 |