Insights into the prokaryotic communities of the abyssal-hadal benthic-boundary layer of the Kuril Kamchatka Trench
Abstract Background The Kuril–Kamchatka Trench (maximum depth 9604 m), located in the NW Pacific Ocean, is among the top seven deepest hadal trenches. The work aimed to investigate the unexplored abyssal-hadal prokaryotic communities of this fascinating, but underrated environment. Results As for th...
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ftdoajarticles:oai:doaj.org/article:eab8c856a80447c5b0ce43541476a22a 2023-09-05T13:20:45+02:00 Insights into the prokaryotic communities of the abyssal-hadal benthic-boundary layer of the Kuril Kamchatka Trench Susanna Gorrasi Andrea Franzetti Angelika Brandt Ulrike Minzlaff Marcella Pasqualetti Massimiliano Fenice 2023-08-01T00:00:00Z https://doi.org/10.1186/s40793-023-00522-9 https://doaj.org/article/eab8c856a80447c5b0ce43541476a22a EN eng BMC https://doi.org/10.1186/s40793-023-00522-9 https://doaj.org/toc/2524-6372 doi:10.1186/s40793-023-00522-9 2524-6372 https://doaj.org/article/eab8c856a80447c5b0ce43541476a22a Environmental Microbiome, Vol 18, Iss 1, Pp 1-18 (2023) Bacterial communities Archaeal communities Deep Sea Abyssal-hadal zone Kuril Kamchatka Trench 16S rRNA gene amplicon sequencing Environmental sciences GE1-350 Microbiology QR1-502 article 2023 ftdoajarticles https://doi.org/10.1186/s40793-023-00522-9 2023-08-13T00:40:35Z Abstract Background The Kuril–Kamchatka Trench (maximum depth 9604 m), located in the NW Pacific Ocean, is among the top seven deepest hadal trenches. The work aimed to investigate the unexplored abyssal-hadal prokaryotic communities of this fascinating, but underrated environment. Results As for the bacterial communities, we found that Proteobacteria (56.1–74.5%), Bacteroidetes (6.5–19.1%), and Actinobacteria (0.9–16.1%) were the most represented bacterial phyla over all samples. Thaumarchaeota (52.9–91.1%) was the most abundant phylum in the archaeal communities. The archaeal diversity was highly represented by the ammonia-oxidizing Nitrosopumilus, and the potential hydrocarbon-degrading bacteria Acinetobacter, Zhongshania, and Colwellia were the main bacterial genera. The α-diversity analysis evidenced that both prokaryotic communities were characterized by low evenness, as indicated by the high Gini index values (> 0.9). The β-diversity analysis (Redundancy Analysis) indicated that, as expected, the depth significantly affected the structure of the prokaryotic communities. The co-occurrence network revealed seven prokaryotic groups that covaried across the abyssal-hadal zone of the Kuril–Kamchatka Trench. Among them, the main group included the most abundant archaeal and bacterial OTUs (Nitrosopumilus OTU A2 and OTU A1; Acinetobacter OTU B1), which were ubiquitous across the trench. Conclusions This manuscript represents the first attempt to characterize the prokaryotic communities of the KKT abyssal-hadal zone. Our results reveal that the most abundant prokaryotes harbored by the abyssal-hadal zone of Kuril–Kamchatka Trench were chemolithotrophic archaea and heterotrophic bacteria, which did not show a distinctive pattern distribution according to depth. In particular, Acinetobacter, Zhongshania, and Colwellia (potential hydrocarbon degraders) were the main bacterial genera, and Nitrosopumilus (ammonia oxidizer) was the dominant representative of the archaeal diversity. Article in Journal/Newspaper Kamchatka Directory of Open Access Journals: DOAJ Articles Pacific Environmental Microbiome 18 1 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Bacterial communities Archaeal communities Deep Sea Abyssal-hadal zone Kuril Kamchatka Trench 16S rRNA gene amplicon sequencing Environmental sciences GE1-350 Microbiology QR1-502 |
spellingShingle |
Bacterial communities Archaeal communities Deep Sea Abyssal-hadal zone Kuril Kamchatka Trench 16S rRNA gene amplicon sequencing Environmental sciences GE1-350 Microbiology QR1-502 Susanna Gorrasi Andrea Franzetti Angelika Brandt Ulrike Minzlaff Marcella Pasqualetti Massimiliano Fenice Insights into the prokaryotic communities of the abyssal-hadal benthic-boundary layer of the Kuril Kamchatka Trench |
topic_facet |
Bacterial communities Archaeal communities Deep Sea Abyssal-hadal zone Kuril Kamchatka Trench 16S rRNA gene amplicon sequencing Environmental sciences GE1-350 Microbiology QR1-502 |
description |
Abstract Background The Kuril–Kamchatka Trench (maximum depth 9604 m), located in the NW Pacific Ocean, is among the top seven deepest hadal trenches. The work aimed to investigate the unexplored abyssal-hadal prokaryotic communities of this fascinating, but underrated environment. Results As for the bacterial communities, we found that Proteobacteria (56.1–74.5%), Bacteroidetes (6.5–19.1%), and Actinobacteria (0.9–16.1%) were the most represented bacterial phyla over all samples. Thaumarchaeota (52.9–91.1%) was the most abundant phylum in the archaeal communities. The archaeal diversity was highly represented by the ammonia-oxidizing Nitrosopumilus, and the potential hydrocarbon-degrading bacteria Acinetobacter, Zhongshania, and Colwellia were the main bacterial genera. The α-diversity analysis evidenced that both prokaryotic communities were characterized by low evenness, as indicated by the high Gini index values (> 0.9). The β-diversity analysis (Redundancy Analysis) indicated that, as expected, the depth significantly affected the structure of the prokaryotic communities. The co-occurrence network revealed seven prokaryotic groups that covaried across the abyssal-hadal zone of the Kuril–Kamchatka Trench. Among them, the main group included the most abundant archaeal and bacterial OTUs (Nitrosopumilus OTU A2 and OTU A1; Acinetobacter OTU B1), which were ubiquitous across the trench. Conclusions This manuscript represents the first attempt to characterize the prokaryotic communities of the KKT abyssal-hadal zone. Our results reveal that the most abundant prokaryotes harbored by the abyssal-hadal zone of Kuril–Kamchatka Trench were chemolithotrophic archaea and heterotrophic bacteria, which did not show a distinctive pattern distribution according to depth. In particular, Acinetobacter, Zhongshania, and Colwellia (potential hydrocarbon degraders) were the main bacterial genera, and Nitrosopumilus (ammonia oxidizer) was the dominant representative of the archaeal diversity. |
format |
Article in Journal/Newspaper |
author |
Susanna Gorrasi Andrea Franzetti Angelika Brandt Ulrike Minzlaff Marcella Pasqualetti Massimiliano Fenice |
author_facet |
Susanna Gorrasi Andrea Franzetti Angelika Brandt Ulrike Minzlaff Marcella Pasqualetti Massimiliano Fenice |
author_sort |
Susanna Gorrasi |
title |
Insights into the prokaryotic communities of the abyssal-hadal benthic-boundary layer of the Kuril Kamchatka Trench |
title_short |
Insights into the prokaryotic communities of the abyssal-hadal benthic-boundary layer of the Kuril Kamchatka Trench |
title_full |
Insights into the prokaryotic communities of the abyssal-hadal benthic-boundary layer of the Kuril Kamchatka Trench |
title_fullStr |
Insights into the prokaryotic communities of the abyssal-hadal benthic-boundary layer of the Kuril Kamchatka Trench |
title_full_unstemmed |
Insights into the prokaryotic communities of the abyssal-hadal benthic-boundary layer of the Kuril Kamchatka Trench |
title_sort |
insights into the prokaryotic communities of the abyssal-hadal benthic-boundary layer of the kuril kamchatka trench |
publisher |
BMC |
publishDate |
2023 |
url |
https://doi.org/10.1186/s40793-023-00522-9 https://doaj.org/article/eab8c856a80447c5b0ce43541476a22a |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Kamchatka |
genre_facet |
Kamchatka |
op_source |
Environmental Microbiome, Vol 18, Iss 1, Pp 1-18 (2023) |
op_relation |
https://doi.org/10.1186/s40793-023-00522-9 https://doaj.org/toc/2524-6372 doi:10.1186/s40793-023-00522-9 2524-6372 https://doaj.org/article/eab8c856a80447c5b0ce43541476a22a |
op_doi |
https://doi.org/10.1186/s40793-023-00522-9 |
container_title |
Environmental Microbiome |
container_volume |
18 |
container_issue |
1 |
_version_ |
1776201388456411136 |