Genome and transcriptome analyses provide insight into the euryhaline adaptation mechanism of Crassostrea gigas.
BACKGROUND: The Pacific oyster, Crassostrea gigas, has developed special mechanisms to regulate its osmotic balance to adapt to fluctuations of salinities in coastal zones. To understand the oyster's euryhaline adaptation, we analyzed salt stress effectors metabolism pathways under different sa...
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ftdoajarticles:oai:doaj.org/article:eaa2f8c729a841a2a79e0b822740651c 2023-05-15T15:58:20+02:00 Genome and transcriptome analyses provide insight into the euryhaline adaptation mechanism of Crassostrea gigas. Jie Meng Qihui Zhu Linlin Zhang Chunyan Li Li Li Zhicai She Baoyu Huang Guofan Zhang 2013-01-01T00:00:00Z https://doi.org/10.1371/journal.pone.0058563 https://doaj.org/article/eaa2f8c729a841a2a79e0b822740651c EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3595286?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0058563 https://doaj.org/article/eaa2f8c729a841a2a79e0b822740651c PLoS ONE, Vol 8, Iss 3, p e58563 (2013) Medicine R Science Q article 2013 ftdoajarticles https://doi.org/10.1371/journal.pone.0058563 2022-12-31T13:19:48Z BACKGROUND: The Pacific oyster, Crassostrea gigas, has developed special mechanisms to regulate its osmotic balance to adapt to fluctuations of salinities in coastal zones. To understand the oyster's euryhaline adaptation, we analyzed salt stress effectors metabolism pathways under different salinities (salt 5, 10, 15, 20, 25, 30 and 40 for 7 days) using transcriptome data, physiology experiment and quantitative real-time PCR. RESULTS: Transcriptome data uncovered 189, 480, 207 and 80 marker genes for monitoring physiology status of oysters and the environment conditions. Three known salt stress effectors (involving ion channels, aquaporins and free amino acids) were examined. The analysis of ion channels and aquaporins indicated that 7 days long-term salt stress inhibited voltage-gated Na(+)/K(+) channel and aquaporin but increased calcium-activated K(+) channel and Ca(2+) channel. As the most important category of osmotic stress effector, we analyzed the oyster FAAs metabolism pathways (including taurine, glycine, alanine, beta-alanine, proline and arginine) and explained FAAs functional mechanism for oyster low salinity adaptation. FAAs metabolism key enzyme genes displayed expression differentiation in low salinity adapted individuals comparing with control which further indicated that FAAs played important roles for oyster salinity adaptation. A global metabolic pathway analysis (iPath) of oyster expanded genes displayed a co-expansion of FAAs metabolism in C. gigas compared with seven other species, suggesting oyster's powerful ability regarding FAAs metabolism, allowing it to adapt to fluctuating salinities, which may be one important mechanism underlying euryhaline adaption in oyster. Additionally, using transcriptome data analysis, we uncovered salt stress transduction networks in C. gigas. CONCLUSIONS: Our results represented oyster salt stress effectors functional mechanisms under salt stress conditions and explained the expansion of FAAs metabolism pathways as the most important effectors for oyster ... Article in Journal/Newspaper Crassostrea gigas Pacific oyster Directory of Open Access Journals: DOAJ Articles Pacific PLoS ONE 8 3 e58563 |
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Directory of Open Access Journals: DOAJ Articles |
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language |
English |
topic |
Medicine R Science Q |
spellingShingle |
Medicine R Science Q Jie Meng Qihui Zhu Linlin Zhang Chunyan Li Li Li Zhicai She Baoyu Huang Guofan Zhang Genome and transcriptome analyses provide insight into the euryhaline adaptation mechanism of Crassostrea gigas. |
topic_facet |
Medicine R Science Q |
description |
BACKGROUND: The Pacific oyster, Crassostrea gigas, has developed special mechanisms to regulate its osmotic balance to adapt to fluctuations of salinities in coastal zones. To understand the oyster's euryhaline adaptation, we analyzed salt stress effectors metabolism pathways under different salinities (salt 5, 10, 15, 20, 25, 30 and 40 for 7 days) using transcriptome data, physiology experiment and quantitative real-time PCR. RESULTS: Transcriptome data uncovered 189, 480, 207 and 80 marker genes for monitoring physiology status of oysters and the environment conditions. Three known salt stress effectors (involving ion channels, aquaporins and free amino acids) were examined. The analysis of ion channels and aquaporins indicated that 7 days long-term salt stress inhibited voltage-gated Na(+)/K(+) channel and aquaporin but increased calcium-activated K(+) channel and Ca(2+) channel. As the most important category of osmotic stress effector, we analyzed the oyster FAAs metabolism pathways (including taurine, glycine, alanine, beta-alanine, proline and arginine) and explained FAAs functional mechanism for oyster low salinity adaptation. FAAs metabolism key enzyme genes displayed expression differentiation in low salinity adapted individuals comparing with control which further indicated that FAAs played important roles for oyster salinity adaptation. A global metabolic pathway analysis (iPath) of oyster expanded genes displayed a co-expansion of FAAs metabolism in C. gigas compared with seven other species, suggesting oyster's powerful ability regarding FAAs metabolism, allowing it to adapt to fluctuating salinities, which may be one important mechanism underlying euryhaline adaption in oyster. Additionally, using transcriptome data analysis, we uncovered salt stress transduction networks in C. gigas. CONCLUSIONS: Our results represented oyster salt stress effectors functional mechanisms under salt stress conditions and explained the expansion of FAAs metabolism pathways as the most important effectors for oyster ... |
format |
Article in Journal/Newspaper |
author |
Jie Meng Qihui Zhu Linlin Zhang Chunyan Li Li Li Zhicai She Baoyu Huang Guofan Zhang |
author_facet |
Jie Meng Qihui Zhu Linlin Zhang Chunyan Li Li Li Zhicai She Baoyu Huang Guofan Zhang |
author_sort |
Jie Meng |
title |
Genome and transcriptome analyses provide insight into the euryhaline adaptation mechanism of Crassostrea gigas. |
title_short |
Genome and transcriptome analyses provide insight into the euryhaline adaptation mechanism of Crassostrea gigas. |
title_full |
Genome and transcriptome analyses provide insight into the euryhaline adaptation mechanism of Crassostrea gigas. |
title_fullStr |
Genome and transcriptome analyses provide insight into the euryhaline adaptation mechanism of Crassostrea gigas. |
title_full_unstemmed |
Genome and transcriptome analyses provide insight into the euryhaline adaptation mechanism of Crassostrea gigas. |
title_sort |
genome and transcriptome analyses provide insight into the euryhaline adaptation mechanism of crassostrea gigas. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2013 |
url |
https://doi.org/10.1371/journal.pone.0058563 https://doaj.org/article/eaa2f8c729a841a2a79e0b822740651c |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
PLoS ONE, Vol 8, Iss 3, p e58563 (2013) |
op_relation |
http://europepmc.org/articles/PMC3595286?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0058563 https://doaj.org/article/eaa2f8c729a841a2a79e0b822740651c |
op_doi |
https://doi.org/10.1371/journal.pone.0058563 |
container_title |
PLoS ONE |
container_volume |
8 |
container_issue |
3 |
container_start_page |
e58563 |
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1766394072076910592 |