Characterization of the differential expressed genes and transcriptomic pathway analysis in the liver of sub-adult red drum (Sciaenops ocellatus) exposed to Deepwater Horizon chemically dispersed oil
The Deepwater Horizon blowout resulted in the second-largest quantity of chemical dispersants used as a countermeasure for an open water oil spill in the Gulf of Mexico. Of which, the efficacy of dispersant as a mitigation strategy and its toxic effects on aquatic fauna remains controversial. To enh...
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ftdoajarticles:oai:doaj.org/article:ea8acbf565ba4c5180849ce3ff10997e 2023-05-15T18:06:01+02:00 Characterization of the differential expressed genes and transcriptomic pathway analysis in the liver of sub-adult red drum (Sciaenops ocellatus) exposed to Deepwater Horizon chemically dispersed oil Tracy A. Sherwood Maria L. Rodgers Andrea M. Tarnecki Dana L. Wetzel 2021-05-01T00:00:00Z https://doi.org/10.1016/j.ecoenv.2021.112098 https://doaj.org/article/ea8acbf565ba4c5180849ce3ff10997e EN eng Elsevier http://www.sciencedirect.com/science/article/pii/S0147651321002098 https://doaj.org/toc/0147-6513 0147-6513 doi:10.1016/j.ecoenv.2021.112098 https://doaj.org/article/ea8acbf565ba4c5180849ce3ff10997e Ecotoxicology and Environmental Safety, Vol 214, Iss , Pp 112098- (2021) Red drum Sciaenops ocellatus Transcriptome RNA-sequencing Dispersed-oil CEWAF Environmental pollution TD172-193.5 Environmental sciences GE1-350 article 2021 ftdoajarticles https://doi.org/10.1016/j.ecoenv.2021.112098 2022-12-31T06:48:34Z The Deepwater Horizon blowout resulted in the second-largest quantity of chemical dispersants used as a countermeasure for an open water oil spill in the Gulf of Mexico. Of which, the efficacy of dispersant as a mitigation strategy and its toxic effects on aquatic fauna remains controversial. To enhance our understanding of potential sub-lethal effects of exposure to chemically dispersed-oil, sub-adult red drum (Sciaenops ocellatus) were continuously exposed to a Corexit 9500: DWH crude oil chemically enhanced water accommodated fraction (CEWAF) for 3-days and transcriptomic responses were assessed in the liver. Differential expressed gene (DEG) analysis demonstrated that 63 genes were significantly impacted in the CEWAF exposed fish. Of these, 37 were upregulated and 26 downregulated. The upregulated genes were primarily involved in metabolism and oxidative stress, whereas several immune genes were downregulated. Quantitative real-time RT-PCR further confirmed upregulation of cytochrome P450 and glutathione S-transferase, along with downregulation of fucolectin 2 and chemokine C-C motif ligand 20. Ingenuity Pathway Analysis (IPA) predicted 120 pathways significantly altered in the CEWAF exposed red drum. The aryl hydrocarbon receptor pathway was significantly activated, while pathways associated with immune and cellular homeostasis were primarily suppressed. The results of this study indicate that CEWAF exposure significantly affects gene expression and alters signaling of biological pathways important in detoxification, immunity, and normal cellular physiology, which can have potential consequences on organismal fitness. Article in Journal/Newspaper Red drum Sciaenops ocellatus Directory of Open Access Journals: DOAJ Articles Ecotoxicology and Environmental Safety 214 112098 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Red drum Sciaenops ocellatus Transcriptome RNA-sequencing Dispersed-oil CEWAF Environmental pollution TD172-193.5 Environmental sciences GE1-350 |
spellingShingle |
Red drum Sciaenops ocellatus Transcriptome RNA-sequencing Dispersed-oil CEWAF Environmental pollution TD172-193.5 Environmental sciences GE1-350 Tracy A. Sherwood Maria L. Rodgers Andrea M. Tarnecki Dana L. Wetzel Characterization of the differential expressed genes and transcriptomic pathway analysis in the liver of sub-adult red drum (Sciaenops ocellatus) exposed to Deepwater Horizon chemically dispersed oil |
topic_facet |
Red drum Sciaenops ocellatus Transcriptome RNA-sequencing Dispersed-oil CEWAF Environmental pollution TD172-193.5 Environmental sciences GE1-350 |
description |
The Deepwater Horizon blowout resulted in the second-largest quantity of chemical dispersants used as a countermeasure for an open water oil spill in the Gulf of Mexico. Of which, the efficacy of dispersant as a mitigation strategy and its toxic effects on aquatic fauna remains controversial. To enhance our understanding of potential sub-lethal effects of exposure to chemically dispersed-oil, sub-adult red drum (Sciaenops ocellatus) were continuously exposed to a Corexit 9500: DWH crude oil chemically enhanced water accommodated fraction (CEWAF) for 3-days and transcriptomic responses were assessed in the liver. Differential expressed gene (DEG) analysis demonstrated that 63 genes were significantly impacted in the CEWAF exposed fish. Of these, 37 were upregulated and 26 downregulated. The upregulated genes were primarily involved in metabolism and oxidative stress, whereas several immune genes were downregulated. Quantitative real-time RT-PCR further confirmed upregulation of cytochrome P450 and glutathione S-transferase, along with downregulation of fucolectin 2 and chemokine C-C motif ligand 20. Ingenuity Pathway Analysis (IPA) predicted 120 pathways significantly altered in the CEWAF exposed red drum. The aryl hydrocarbon receptor pathway was significantly activated, while pathways associated with immune and cellular homeostasis were primarily suppressed. The results of this study indicate that CEWAF exposure significantly affects gene expression and alters signaling of biological pathways important in detoxification, immunity, and normal cellular physiology, which can have potential consequences on organismal fitness. |
format |
Article in Journal/Newspaper |
author |
Tracy A. Sherwood Maria L. Rodgers Andrea M. Tarnecki Dana L. Wetzel |
author_facet |
Tracy A. Sherwood Maria L. Rodgers Andrea M. Tarnecki Dana L. Wetzel |
author_sort |
Tracy A. Sherwood |
title |
Characterization of the differential expressed genes and transcriptomic pathway analysis in the liver of sub-adult red drum (Sciaenops ocellatus) exposed to Deepwater Horizon chemically dispersed oil |
title_short |
Characterization of the differential expressed genes and transcriptomic pathway analysis in the liver of sub-adult red drum (Sciaenops ocellatus) exposed to Deepwater Horizon chemically dispersed oil |
title_full |
Characterization of the differential expressed genes and transcriptomic pathway analysis in the liver of sub-adult red drum (Sciaenops ocellatus) exposed to Deepwater Horizon chemically dispersed oil |
title_fullStr |
Characterization of the differential expressed genes and transcriptomic pathway analysis in the liver of sub-adult red drum (Sciaenops ocellatus) exposed to Deepwater Horizon chemically dispersed oil |
title_full_unstemmed |
Characterization of the differential expressed genes and transcriptomic pathway analysis in the liver of sub-adult red drum (Sciaenops ocellatus) exposed to Deepwater Horizon chemically dispersed oil |
title_sort |
characterization of the differential expressed genes and transcriptomic pathway analysis in the liver of sub-adult red drum (sciaenops ocellatus) exposed to deepwater horizon chemically dispersed oil |
publisher |
Elsevier |
publishDate |
2021 |
url |
https://doi.org/10.1016/j.ecoenv.2021.112098 https://doaj.org/article/ea8acbf565ba4c5180849ce3ff10997e |
genre |
Red drum Sciaenops ocellatus |
genre_facet |
Red drum Sciaenops ocellatus |
op_source |
Ecotoxicology and Environmental Safety, Vol 214, Iss , Pp 112098- (2021) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S0147651321002098 https://doaj.org/toc/0147-6513 0147-6513 doi:10.1016/j.ecoenv.2021.112098 https://doaj.org/article/ea8acbf565ba4c5180849ce3ff10997e |
op_doi |
https://doi.org/10.1016/j.ecoenv.2021.112098 |
container_title |
Ecotoxicology and Environmental Safety |
container_volume |
214 |
container_start_page |
112098 |
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1766177582270644224 |