Proteomic analysis of adult Ascaris suum fluid compartments and secretory products.
Strategies employed by parasites to establish infections are poorly understood. The host-parasite interface is maintained through a molecular dialog that, among other roles, protects parasites from host immune responses. Parasite excretory/secretory products (ESP) play major roles in this process. U...
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ftdoajarticles:oai:doaj.org/article:ea7bac288037479fb2509a35276e6a19 2023-05-15T15:14:46+02:00 Proteomic analysis of adult Ascaris suum fluid compartments and secretory products. James F Chehayeb Alan P Robertson Richard J Martin Timothy G Geary 2014-06-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0002939 https://doaj.org/article/ea7bac288037479fb2509a35276e6a19 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4046973?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0002939 https://doaj.org/article/ea7bac288037479fb2509a35276e6a19 PLoS Neglected Tropical Diseases, Vol 8, Iss 6, p e2939 (2014) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2014 ftdoajarticles https://doi.org/10.1371/journal.pntd.0002939 2022-12-31T14:37:39Z Strategies employed by parasites to establish infections are poorly understood. The host-parasite interface is maintained through a molecular dialog that, among other roles, protects parasites from host immune responses. Parasite excretory/secretory products (ESP) play major roles in this process. Understanding the biology of protein secretion by parasites and their associated functional processes will enhance our understanding of the roles of ESP in host-parasite interactions.ESP was collected after culturing 10 adult female Ascaris suum. Perienteric fluid (PE) and uterine fluid (UF) were collected directly from adult females by dissection. Using SDS-PAGE coupled with LC-MS/MS, we identified 175, 308 and 274 proteins in ESP, PE and UF, respectively. Although many proteins were shared among the samples, the protein composition of ESP was distinct from PE and UF, whereas PE and UF were highly similar. The distribution of gene ontology (GO) terms for proteins in ESP, PE and UF supports this claim. Comparison of ESP composition in A. suum, Brugia malayi and Heligmosoides polygyrus showed that proteins found in UF were also secreted by males and by larval stages of other species, suggesting that multiple routes of secretion may be used for homologous proteins. ESP composition of nematodes is both phylogeny- and niche-dependent.Analysis of the protein composition of A. suum ESP and UF leads to the conclusion that the excretory-secretory apparatus and uterus are separate routes for protein release. Proteins detected in ESP have distinct patterns of biological functions compared to those in UF. PE is likely to serve as the source of the majority of proteins in UF. This analysis expands our knowledge of the biology of protein secretion from nematodes and will inform new studies on the function of secreted proteins in the orchestration of host-parasite interactions. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 8 6 e2939 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 James F Chehayeb Alan P Robertson Richard J Martin Timothy G Geary Proteomic analysis of adult Ascaris suum fluid compartments and secretory products. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Strategies employed by parasites to establish infections are poorly understood. The host-parasite interface is maintained through a molecular dialog that, among other roles, protects parasites from host immune responses. Parasite excretory/secretory products (ESP) play major roles in this process. Understanding the biology of protein secretion by parasites and their associated functional processes will enhance our understanding of the roles of ESP in host-parasite interactions.ESP was collected after culturing 10 adult female Ascaris suum. Perienteric fluid (PE) and uterine fluid (UF) were collected directly from adult females by dissection. Using SDS-PAGE coupled with LC-MS/MS, we identified 175, 308 and 274 proteins in ESP, PE and UF, respectively. Although many proteins were shared among the samples, the protein composition of ESP was distinct from PE and UF, whereas PE and UF were highly similar. The distribution of gene ontology (GO) terms for proteins in ESP, PE and UF supports this claim. Comparison of ESP composition in A. suum, Brugia malayi and Heligmosoides polygyrus showed that proteins found in UF were also secreted by males and by larval stages of other species, suggesting that multiple routes of secretion may be used for homologous proteins. ESP composition of nematodes is both phylogeny- and niche-dependent.Analysis of the protein composition of A. suum ESP and UF leads to the conclusion that the excretory-secretory apparatus and uterus are separate routes for protein release. Proteins detected in ESP have distinct patterns of biological functions compared to those in UF. PE is likely to serve as the source of the majority of proteins in UF. This analysis expands our knowledge of the biology of protein secretion from nematodes and will inform new studies on the function of secreted proteins in the orchestration of host-parasite interactions. |
format |
Article in Journal/Newspaper |
author |
James F Chehayeb Alan P Robertson Richard J Martin Timothy G Geary |
author_facet |
James F Chehayeb Alan P Robertson Richard J Martin Timothy G Geary |
author_sort |
James F Chehayeb |
title |
Proteomic analysis of adult Ascaris suum fluid compartments and secretory products. |
title_short |
Proteomic analysis of adult Ascaris suum fluid compartments and secretory products. |
title_full |
Proteomic analysis of adult Ascaris suum fluid compartments and secretory products. |
title_fullStr |
Proteomic analysis of adult Ascaris suum fluid compartments and secretory products. |
title_full_unstemmed |
Proteomic analysis of adult Ascaris suum fluid compartments and secretory products. |
title_sort |
proteomic analysis of adult ascaris suum fluid compartments and secretory products. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2014 |
url |
https://doi.org/10.1371/journal.pntd.0002939 https://doaj.org/article/ea7bac288037479fb2509a35276e6a19 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 8, Iss 6, p e2939 (2014) |
op_relation |
http://europepmc.org/articles/PMC4046973?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0002939 https://doaj.org/article/ea7bac288037479fb2509a35276e6a19 |
op_doi |
https://doi.org/10.1371/journal.pntd.0002939 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
8 |
container_issue |
6 |
container_start_page |
e2939 |
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1766345188400168960 |