The Antimicrobial Peptide Cecropin AD Supplement Alleviated Soybean Meal-Induced Intestinal Inflammation, Barrier Damage, and Microbial Dysbiosis in Juvenile Turbot, Scophthalmus maximus
This study aimed to investigate the benefit of dietary cecropin AD (CAD) on the intestinal health of turbot (Scophthalmus maximus) fed diets with a high level of soybean meal. A 12-week feeding trial was conducted with four isonitrogenous and isolipidic diets: a fishmeal-based diet (FM), a diet repl...
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ftdoajarticles:oai:doaj.org/article:ea121e5132524e4f819f2d5347fae97b 2023-05-15T18:15:45+02:00 The Antimicrobial Peptide Cecropin AD Supplement Alleviated Soybean Meal-Induced Intestinal Inflammation, Barrier Damage, and Microbial Dysbiosis in Juvenile Turbot, Scophthalmus maximus Jihong Dai Weihao Ou Guijuan Yu Qinghui Ai Wenbing Zhang Kangsen Mai Yanjiao Zhang 2020-11-01T00:00:00Z https://doi.org/10.3389/fmars.2020.584482 https://doaj.org/article/ea121e5132524e4f819f2d5347fae97b EN eng Frontiers Media S.A. https://www.frontiersin.org/articles/10.3389/fmars.2020.584482/full https://doaj.org/toc/2296-7745 2296-7745 doi:10.3389/fmars.2020.584482 https://doaj.org/article/ea121e5132524e4f819f2d5347fae97b Frontiers in Marine Science, Vol 7 (2020) cecropin AD intestinal inflammation intestinal tight junction intestinal microbiota turbot Science Q General. Including nature conservation geographical distribution QH1-199.5 article 2020 ftdoajarticles https://doi.org/10.3389/fmars.2020.584482 2022-12-31T12:11:54Z This study aimed to investigate the benefit of dietary cecropin AD (CAD) on the intestinal health of turbot (Scophthalmus maximus) fed diets with a high level of soybean meal. A 12-week feeding trial was conducted with four isonitrogenous and isolipidic diets: a fishmeal-based diet (FM), a diet replacing 40% fish meal protein of FM diet with soybean meal protein (SBM), and the SBM diet supplemented with 0.5 g/kg (C1) and 1.0 g/kg (C2) CAD, respectively. The results of histology of distal intestine (DI) showed that turbots fed the SBM diet exhibited distinct symptoms of enteritis. However, fish fed diets with CAD supplementation kept the normal appearance of the DI which was similar to that in FM group. Compared with the SBM group, diet with CAD supplementation significantly decreased the gene expression of intestinal pro-inflammatory cytokines tumor necrosis factor-α (tnf-α), interleukin-1β (il-1β), interferon-gamma (ifn-γ), and nuclear factor-kappa B p65 (nf-κb p65), while up-regulated the gene expression of intestinal tight junction proteins claudin-3, claudin-4, occludin, and zonula occludens-1 (zo-1). Besides, diet C1 shaped the intestinal microbiota profile toward an anti-inflammatory phenotype represented by the increased abundance of Blutia, Firmicutes/Bacteroides ratio, and decreased Prevotellaceae. In conclusion, dietary CAD could positively modulate the intestinal health of turbot from the impairment induced by soybean meal, which expands its application to help fish better adapt to the increasing plant protein level in aquafeed. Article in Journal/Newspaper Scophthalmus maximus Turbot Directory of Open Access Journals: DOAJ Articles Frontiers in Marine Science 7 |
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op_collection_id |
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English |
topic |
cecropin AD intestinal inflammation intestinal tight junction intestinal microbiota turbot Science Q General. Including nature conservation geographical distribution QH1-199.5 |
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cecropin AD intestinal inflammation intestinal tight junction intestinal microbiota turbot Science Q General. Including nature conservation geographical distribution QH1-199.5 Jihong Dai Weihao Ou Guijuan Yu Qinghui Ai Wenbing Zhang Kangsen Mai Yanjiao Zhang The Antimicrobial Peptide Cecropin AD Supplement Alleviated Soybean Meal-Induced Intestinal Inflammation, Barrier Damage, and Microbial Dysbiosis in Juvenile Turbot, Scophthalmus maximus |
topic_facet |
cecropin AD intestinal inflammation intestinal tight junction intestinal microbiota turbot Science Q General. Including nature conservation geographical distribution QH1-199.5 |
description |
This study aimed to investigate the benefit of dietary cecropin AD (CAD) on the intestinal health of turbot (Scophthalmus maximus) fed diets with a high level of soybean meal. A 12-week feeding trial was conducted with four isonitrogenous and isolipidic diets: a fishmeal-based diet (FM), a diet replacing 40% fish meal protein of FM diet with soybean meal protein (SBM), and the SBM diet supplemented with 0.5 g/kg (C1) and 1.0 g/kg (C2) CAD, respectively. The results of histology of distal intestine (DI) showed that turbots fed the SBM diet exhibited distinct symptoms of enteritis. However, fish fed diets with CAD supplementation kept the normal appearance of the DI which was similar to that in FM group. Compared with the SBM group, diet with CAD supplementation significantly decreased the gene expression of intestinal pro-inflammatory cytokines tumor necrosis factor-α (tnf-α), interleukin-1β (il-1β), interferon-gamma (ifn-γ), and nuclear factor-kappa B p65 (nf-κb p65), while up-regulated the gene expression of intestinal tight junction proteins claudin-3, claudin-4, occludin, and zonula occludens-1 (zo-1). Besides, diet C1 shaped the intestinal microbiota profile toward an anti-inflammatory phenotype represented by the increased abundance of Blutia, Firmicutes/Bacteroides ratio, and decreased Prevotellaceae. In conclusion, dietary CAD could positively modulate the intestinal health of turbot from the impairment induced by soybean meal, which expands its application to help fish better adapt to the increasing plant protein level in aquafeed. |
format |
Article in Journal/Newspaper |
author |
Jihong Dai Weihao Ou Guijuan Yu Qinghui Ai Wenbing Zhang Kangsen Mai Yanjiao Zhang |
author_facet |
Jihong Dai Weihao Ou Guijuan Yu Qinghui Ai Wenbing Zhang Kangsen Mai Yanjiao Zhang |
author_sort |
Jihong Dai |
title |
The Antimicrobial Peptide Cecropin AD Supplement Alleviated Soybean Meal-Induced Intestinal Inflammation, Barrier Damage, and Microbial Dysbiosis in Juvenile Turbot, Scophthalmus maximus |
title_short |
The Antimicrobial Peptide Cecropin AD Supplement Alleviated Soybean Meal-Induced Intestinal Inflammation, Barrier Damage, and Microbial Dysbiosis in Juvenile Turbot, Scophthalmus maximus |
title_full |
The Antimicrobial Peptide Cecropin AD Supplement Alleviated Soybean Meal-Induced Intestinal Inflammation, Barrier Damage, and Microbial Dysbiosis in Juvenile Turbot, Scophthalmus maximus |
title_fullStr |
The Antimicrobial Peptide Cecropin AD Supplement Alleviated Soybean Meal-Induced Intestinal Inflammation, Barrier Damage, and Microbial Dysbiosis in Juvenile Turbot, Scophthalmus maximus |
title_full_unstemmed |
The Antimicrobial Peptide Cecropin AD Supplement Alleviated Soybean Meal-Induced Intestinal Inflammation, Barrier Damage, and Microbial Dysbiosis in Juvenile Turbot, Scophthalmus maximus |
title_sort |
antimicrobial peptide cecropin ad supplement alleviated soybean meal-induced intestinal inflammation, barrier damage, and microbial dysbiosis in juvenile turbot, scophthalmus maximus |
publisher |
Frontiers Media S.A. |
publishDate |
2020 |
url |
https://doi.org/10.3389/fmars.2020.584482 https://doaj.org/article/ea121e5132524e4f819f2d5347fae97b |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_source |
Frontiers in Marine Science, Vol 7 (2020) |
op_relation |
https://www.frontiersin.org/articles/10.3389/fmars.2020.584482/full https://doaj.org/toc/2296-7745 2296-7745 doi:10.3389/fmars.2020.584482 https://doaj.org/article/ea121e5132524e4f819f2d5347fae97b |
op_doi |
https://doi.org/10.3389/fmars.2020.584482 |
container_title |
Frontiers in Marine Science |
container_volume |
7 |
_version_ |
1766188966596313088 |