Optimization of the Sybr Green real time PCR for the detection of Human Herpes Virus type 6 (HHV-6) Otimização da PCR em tempo real - Sybr Green para detecção do Herpes Vírus Humano tipo 6 (HHV-6)

• Published in: Revista do Instituto de Medicina Tropical de São Paulo
• Main Authors: Cynthia Liliane Motta do Canto, Laura Massami Sumita, Adriana Freire Machado, Adriana Tateno, Eveline Vieira da Cunha, Clarisse Martins Machado
• Format: Article in Journal/Newspaper
• Language: English
• Published: Universidade de São Paulo (USP) 2008
• Subjects: Human herpes virus 6 (HHV-6); PCR; Real-time PCR; Sybr Green; Arctic medicine. Tropical medicine; RC955-962; Infectious and parasitic diseases; RC109-216; Arctic; Aguda
• Online Access: https://doi.org/10.1590/S0036-46652008000100014; https://doaj.org/article/ea0ea6e185cc4f12969978e4f8d89f5e

HHV-6 is the etiological agent of Exanthem subitum which is considered the sixth most frequent disease in infancy. In immuno-compromised hosts, reactivation of latent HHV-6 infection may cause severe acute disease. We developed a Sybr Green Real Time PCR for HHV-6 and compared the results with neste...