In vitro model of postoncosphere development, and in vivo infection abilities of Taenia solium and Taenia saginata.
Taenia solium is known to cause human cysticercosis while T. saginata does not. Comparative in vitro and in vivo studies on the oncosphere and the postoncospheral (PO) forms of T. solium and T. saginata may help to elucidate why cysticercosis can occur from one and not the other. The aim of this stu...
Published in: | PLOS Neglected Tropical Diseases |
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2019
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ftdoajarticles:oai:doaj.org/article:e9a943025586458b9d593bfc3ac6fbfb 2023-05-15T15:18:40+02:00 In vitro model of postoncosphere development, and in vivo infection abilities of Taenia solium and Taenia saginata. Sandra Palma Nancy Chile Rogger P Carmen-Orozco Grace Trompeter Kayla Fishbeck Virginia Cooper Laura Rapoport Edson G Bernal-Teran Beth J Condori Robert H Gilman Manuela R Verastegui Cysticercosis Working Group in Peru 2019-03-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0007261 https://doaj.org/article/e9a943025586458b9d593bfc3ac6fbfb EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0007261 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007261 https://doaj.org/article/e9a943025586458b9d593bfc3ac6fbfb PLoS Neglected Tropical Diseases, Vol 13, Iss 3, p e0007261 (2019) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2019 ftdoajarticles https://doi.org/10.1371/journal.pntd.0007261 2022-12-31T14:48:49Z Taenia solium is known to cause human cysticercosis while T. saginata does not. Comparative in vitro and in vivo studies on the oncosphere and the postoncospheral (PO) forms of T. solium and T. saginata may help to elucidate why cysticercosis can occur from one and not the other. The aim of this study was to use in vitro culture assays and in vivo models to study the differences in the development of the T. solium and T. saginata oncosphere. Furthermore, this study aimed to evaluate the expression of cytokines and metalloproteinases (MMPs) in human peripheral blood mononuclear cells (PBMCs), which were stimulated by these oncospheres and PO antigens. T. solium and T. saginata activated oncospheres (AO) were cultured in INT-407 and HCT-8 intestinal cells for 180 days. The T. solium began to die while the T. saginata grew for 180 days and developed to cysticerci in INT-407 cells. Rats were inoculated intracranially with AO and PO forms of either T. saginata or T. solium. Rats infected with T. solium AO and PO forms developed neurocysticercosis (NCC), while those infected with the T. saginata did not. Human PMBCs were stimulated with antigens of AO and PO forms of both species, and the production of cytokines and metalloproteinases (MMPs) was measured. The T. solium AO antigen stimulated a higher production of IL-4, IL-5, IL-13, IFN-γ, and IL-2 cytokines compared to T. saginata AO. In the PO form, the T. saginata PO antigen increased the production of IL-4, IL-5, IL-13, IFN-γ, IL-1β, IL-6, IL-10, TNF-α and IL-12 cytokines compared to T. solium, suggesting that this global immune response stimulated by different forms could permit survival or destruction of the parasite depending of their life-cycle stage. Regarding MMPs, T. solium AO antigen stimulated a higher production of MMP-9 compared to T. saginata AO antigen, which may be responsible for altering the permeability of intestinal cells and facilitating breakdown of the blood-brain barrier during the process of invasion of host tissue. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 13 3 e0007261 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Sandra Palma Nancy Chile Rogger P Carmen-Orozco Grace Trompeter Kayla Fishbeck Virginia Cooper Laura Rapoport Edson G Bernal-Teran Beth J Condori Robert H Gilman Manuela R Verastegui Cysticercosis Working Group in Peru In vitro model of postoncosphere development, and in vivo infection abilities of Taenia solium and Taenia saginata. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Taenia solium is known to cause human cysticercosis while T. saginata does not. Comparative in vitro and in vivo studies on the oncosphere and the postoncospheral (PO) forms of T. solium and T. saginata may help to elucidate why cysticercosis can occur from one and not the other. The aim of this study was to use in vitro culture assays and in vivo models to study the differences in the development of the T. solium and T. saginata oncosphere. Furthermore, this study aimed to evaluate the expression of cytokines and metalloproteinases (MMPs) in human peripheral blood mononuclear cells (PBMCs), which were stimulated by these oncospheres and PO antigens. T. solium and T. saginata activated oncospheres (AO) were cultured in INT-407 and HCT-8 intestinal cells for 180 days. The T. solium began to die while the T. saginata grew for 180 days and developed to cysticerci in INT-407 cells. Rats were inoculated intracranially with AO and PO forms of either T. saginata or T. solium. Rats infected with T. solium AO and PO forms developed neurocysticercosis (NCC), while those infected with the T. saginata did not. Human PMBCs were stimulated with antigens of AO and PO forms of both species, and the production of cytokines and metalloproteinases (MMPs) was measured. The T. solium AO antigen stimulated a higher production of IL-4, IL-5, IL-13, IFN-γ, and IL-2 cytokines compared to T. saginata AO. In the PO form, the T. saginata PO antigen increased the production of IL-4, IL-5, IL-13, IFN-γ, IL-1β, IL-6, IL-10, TNF-α and IL-12 cytokines compared to T. solium, suggesting that this global immune response stimulated by different forms could permit survival or destruction of the parasite depending of their life-cycle stage. Regarding MMPs, T. solium AO antigen stimulated a higher production of MMP-9 compared to T. saginata AO antigen, which may be responsible for altering the permeability of intestinal cells and facilitating breakdown of the blood-brain barrier during the process of invasion of host tissue. |
format |
Article in Journal/Newspaper |
author |
Sandra Palma Nancy Chile Rogger P Carmen-Orozco Grace Trompeter Kayla Fishbeck Virginia Cooper Laura Rapoport Edson G Bernal-Teran Beth J Condori Robert H Gilman Manuela R Verastegui Cysticercosis Working Group in Peru |
author_facet |
Sandra Palma Nancy Chile Rogger P Carmen-Orozco Grace Trompeter Kayla Fishbeck Virginia Cooper Laura Rapoport Edson G Bernal-Teran Beth J Condori Robert H Gilman Manuela R Verastegui Cysticercosis Working Group in Peru |
author_sort |
Sandra Palma |
title |
In vitro model of postoncosphere development, and in vivo infection abilities of Taenia solium and Taenia saginata. |
title_short |
In vitro model of postoncosphere development, and in vivo infection abilities of Taenia solium and Taenia saginata. |
title_full |
In vitro model of postoncosphere development, and in vivo infection abilities of Taenia solium and Taenia saginata. |
title_fullStr |
In vitro model of postoncosphere development, and in vivo infection abilities of Taenia solium and Taenia saginata. |
title_full_unstemmed |
In vitro model of postoncosphere development, and in vivo infection abilities of Taenia solium and Taenia saginata. |
title_sort |
in vitro model of postoncosphere development, and in vivo infection abilities of taenia solium and taenia saginata. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2019 |
url |
https://doi.org/10.1371/journal.pntd.0007261 https://doaj.org/article/e9a943025586458b9d593bfc3ac6fbfb |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 13, Iss 3, p e0007261 (2019) |
op_relation |
https://doi.org/10.1371/journal.pntd.0007261 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007261 https://doaj.org/article/e9a943025586458b9d593bfc3ac6fbfb |
op_doi |
https://doi.org/10.1371/journal.pntd.0007261 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
13 |
container_issue |
3 |
container_start_page |
e0007261 |
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1766348861660463104 |