Zika virus disrupts gene expression in human myoblasts and myotubes: Relationship with susceptibility to infection.
The tropism of Zika virus (ZIKV) has been described in the nervous system, blood, placenta, thymus, and skeletal muscle. We investigated the mechanisms of skeletal muscle susceptibility to ZIKV using an in vitro model of human skeletal muscle myogenesis, in which myoblasts differentiate into myotube...
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ftdoajarticles:oai:doaj.org/article:e983be5924664602b8952f9d16616a5c 2023-05-15T15:09:28+02:00 Zika virus disrupts gene expression in human myoblasts and myotubes: Relationship with susceptibility to infection. Ingo Riederer Daniella Arêas Mendes-da-Cruz Guilherme Cordenonsi da Fonseca Mariela Natacha González Otavio Brustolini Cássia Rocha Guilherme Loss Joseane Biso de Carvalho Mariane Talon Menezes Lidiane Menezes Souza Raphael Alexandra Gerber Myrna Cristina Bonaldo Gillian Butler-Browne Vincent Mouly Vinicius Cotta-de-Almeida Wilson Savino Ana Tereza Ribeiro de Vasconcelos 2022-02-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0010166 https://doaj.org/article/e983be5924664602b8952f9d16616a5c EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0010166 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0010166 https://doaj.org/article/e983be5924664602b8952f9d16616a5c PLoS Neglected Tropical Diseases, Vol 16, Iss 2, p e0010166 (2022) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2022 ftdoajarticles https://doi.org/10.1371/journal.pntd.0010166 2022-12-31T03:26:57Z The tropism of Zika virus (ZIKV) has been described in the nervous system, blood, placenta, thymus, and skeletal muscle. We investigated the mechanisms of skeletal muscle susceptibility to ZIKV using an in vitro model of human skeletal muscle myogenesis, in which myoblasts differentiate into myotubes. Myoblasts were permissive to ZIKV infection, generating productive viral particles, while myotubes controlled ZIKV replication. To investigate the underlying mechanisms, we used gene expression profiling. First, we assessed gene changes in myotubes compared with myoblasts in the model without infection. As expected, we observed an increase in genes and pathways related to the contractile muscle system in the myotubes, a reduction in processes linked to proliferation, migration and cytokine production, among others, confirming the myogenic capacity of our system in vitro. A comparison between non-infected and infected myoblasts revealed more than 500 differentially expressed genes (DEGs). In contrast, infected myotubes showed almost 2,000 DEGs, among which we detected genes and pathways highly or exclusively expressed in myotubes, including those related to antiviral and innate immune responses. Such gene modulation could explain our findings showing that ZIKV also invades myotubes but does not replicate in these differentiated cells. In conclusion, we showed that ZIKV largely (but differentially) disrupts gene expression in human myoblasts and myotubes. Identifying genes involved in myotube resistance can shed light on potential antiviral mechanisms against ZIKV infection. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 16 2 e0010166 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Ingo Riederer Daniella Arêas Mendes-da-Cruz Guilherme Cordenonsi da Fonseca Mariela Natacha González Otavio Brustolini Cássia Rocha Guilherme Loss Joseane Biso de Carvalho Mariane Talon Menezes Lidiane Menezes Souza Raphael Alexandra Gerber Myrna Cristina Bonaldo Gillian Butler-Browne Vincent Mouly Vinicius Cotta-de-Almeida Wilson Savino Ana Tereza Ribeiro de Vasconcelos Zika virus disrupts gene expression in human myoblasts and myotubes: Relationship with susceptibility to infection. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
The tropism of Zika virus (ZIKV) has been described in the nervous system, blood, placenta, thymus, and skeletal muscle. We investigated the mechanisms of skeletal muscle susceptibility to ZIKV using an in vitro model of human skeletal muscle myogenesis, in which myoblasts differentiate into myotubes. Myoblasts were permissive to ZIKV infection, generating productive viral particles, while myotubes controlled ZIKV replication. To investigate the underlying mechanisms, we used gene expression profiling. First, we assessed gene changes in myotubes compared with myoblasts in the model without infection. As expected, we observed an increase in genes and pathways related to the contractile muscle system in the myotubes, a reduction in processes linked to proliferation, migration and cytokine production, among others, confirming the myogenic capacity of our system in vitro. A comparison between non-infected and infected myoblasts revealed more than 500 differentially expressed genes (DEGs). In contrast, infected myotubes showed almost 2,000 DEGs, among which we detected genes and pathways highly or exclusively expressed in myotubes, including those related to antiviral and innate immune responses. Such gene modulation could explain our findings showing that ZIKV also invades myotubes but does not replicate in these differentiated cells. In conclusion, we showed that ZIKV largely (but differentially) disrupts gene expression in human myoblasts and myotubes. Identifying genes involved in myotube resistance can shed light on potential antiviral mechanisms against ZIKV infection. |
format |
Article in Journal/Newspaper |
author |
Ingo Riederer Daniella Arêas Mendes-da-Cruz Guilherme Cordenonsi da Fonseca Mariela Natacha González Otavio Brustolini Cássia Rocha Guilherme Loss Joseane Biso de Carvalho Mariane Talon Menezes Lidiane Menezes Souza Raphael Alexandra Gerber Myrna Cristina Bonaldo Gillian Butler-Browne Vincent Mouly Vinicius Cotta-de-Almeida Wilson Savino Ana Tereza Ribeiro de Vasconcelos |
author_facet |
Ingo Riederer Daniella Arêas Mendes-da-Cruz Guilherme Cordenonsi da Fonseca Mariela Natacha González Otavio Brustolini Cássia Rocha Guilherme Loss Joseane Biso de Carvalho Mariane Talon Menezes Lidiane Menezes Souza Raphael Alexandra Gerber Myrna Cristina Bonaldo Gillian Butler-Browne Vincent Mouly Vinicius Cotta-de-Almeida Wilson Savino Ana Tereza Ribeiro de Vasconcelos |
author_sort |
Ingo Riederer |
title |
Zika virus disrupts gene expression in human myoblasts and myotubes: Relationship with susceptibility to infection. |
title_short |
Zika virus disrupts gene expression in human myoblasts and myotubes: Relationship with susceptibility to infection. |
title_full |
Zika virus disrupts gene expression in human myoblasts and myotubes: Relationship with susceptibility to infection. |
title_fullStr |
Zika virus disrupts gene expression in human myoblasts and myotubes: Relationship with susceptibility to infection. |
title_full_unstemmed |
Zika virus disrupts gene expression in human myoblasts and myotubes: Relationship with susceptibility to infection. |
title_sort |
zika virus disrupts gene expression in human myoblasts and myotubes: relationship with susceptibility to infection. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2022 |
url |
https://doi.org/10.1371/journal.pntd.0010166 https://doaj.org/article/e983be5924664602b8952f9d16616a5c |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 16, Iss 2, p e0010166 (2022) |
op_relation |
https://doi.org/10.1371/journal.pntd.0010166 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0010166 https://doaj.org/article/e983be5924664602b8952f9d16616a5c |
op_doi |
https://doi.org/10.1371/journal.pntd.0010166 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
16 |
container_issue |
2 |
container_start_page |
e0010166 |
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1766340661083111424 |