Prion protein gene (PRNP) variation in German and Danish cervids
Abstract The structure of cellular prion proteins encoded by the prion protein gene (PRNP) impacts susceptibility to transmissible spongiform encephalopathies, including chronic wasting disease (CWD) in deer. The recent emergence of CWD in Northern European reindeer (Rangifer tarandus), moose (Alces...
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ftdoajarticles:oai:doaj.org/article:e948b18743cb4d5abc36dd15412c251c 2024-09-09T18:56:33+00:00 Prion protein gene (PRNP) variation in German and Danish cervids Sonja Ernst Agata Piestrzyńska-Kajtoch Jörn Gethmann Małgorzata Natonek-Wiśniewska Balal Sadeghi Miroslaw P. Polak Markus Keller Dolores Gavier-Widén Katayoun Moazami-Goudarzi Fiona Houston Martin H. Groschup Christine Fast 2024-08-01T00:00:00Z https://doi.org/10.1186/s13567-024-01340-8 https://doaj.org/article/e948b18743cb4d5abc36dd15412c251c EN eng BMC https://doi.org/10.1186/s13567-024-01340-8 https://doaj.org/toc/1297-9716 doi:10.1186/s13567-024-01340-8 1297-9716 https://doaj.org/article/e948b18743cb4d5abc36dd15412c251c Veterinary Research, Vol 55, Iss 1, Pp 1-17 (2024) Chronic wasting disease deer PRNP genotype Germany polymorphism Veterinary medicine SF600-1100 article 2024 ftdoajarticles https://doi.org/10.1186/s13567-024-01340-8 2024-08-05T17:50:07Z Abstract The structure of cellular prion proteins encoded by the prion protein gene (PRNP) impacts susceptibility to transmissible spongiform encephalopathies, including chronic wasting disease (CWD) in deer. The recent emergence of CWD in Northern European reindeer (Rangifer tarandus), moose (Alces alces alces) and red deer (Cervus elaphus), in parallel with the outbreak in North America, gives reason to investigate PRNP variation in European deer, to implement risk assessments and adjust CWD management for deer populations under threat. We here report PRNP-sequence data from 911 samples of German red, roe (Capreolus capreolus), sika (Cervus nippon) and fallow deer (Dama dama) as well as additional data from 26 Danish red deer close to the German border and four zoo species not native to Germany. No PRNP sequence variation was observed in roe and fallow deer, as previously described for populations across Europe. In contrast, a broad PRNP variation was detected in red deer, with non-synonymous polymorphisms at codons 98, 226 and 247 as well as synonymous mutations at codons 21, 78, 136 and 185. Moreover, a novel 24 bp deletion within the octapeptide repeat was detected. In summary, 14 genotypes were seen in red deer with significant differences in their geographical distribution and frequencies, including geographical clustering of certain genotypes, suggesting “PRNP-linages” in this species. Based on data from North American CWD and the genotyping results of the European CWD cases, we would predict that large proportions of wild cervids in Europe might be susceptible to CWD once introduced to naive populations. Article in Journal/Newspaper Alces alces Rangifer tarandus Directory of Open Access Journals: DOAJ Articles Veterinary Research 55 1 |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Chronic wasting disease deer PRNP genotype Germany polymorphism Veterinary medicine SF600-1100 |
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Chronic wasting disease deer PRNP genotype Germany polymorphism Veterinary medicine SF600-1100 Sonja Ernst Agata Piestrzyńska-Kajtoch Jörn Gethmann Małgorzata Natonek-Wiśniewska Balal Sadeghi Miroslaw P. Polak Markus Keller Dolores Gavier-Widén Katayoun Moazami-Goudarzi Fiona Houston Martin H. Groschup Christine Fast Prion protein gene (PRNP) variation in German and Danish cervids |
topic_facet |
Chronic wasting disease deer PRNP genotype Germany polymorphism Veterinary medicine SF600-1100 |
description |
Abstract The structure of cellular prion proteins encoded by the prion protein gene (PRNP) impacts susceptibility to transmissible spongiform encephalopathies, including chronic wasting disease (CWD) in deer. The recent emergence of CWD in Northern European reindeer (Rangifer tarandus), moose (Alces alces alces) and red deer (Cervus elaphus), in parallel with the outbreak in North America, gives reason to investigate PRNP variation in European deer, to implement risk assessments and adjust CWD management for deer populations under threat. We here report PRNP-sequence data from 911 samples of German red, roe (Capreolus capreolus), sika (Cervus nippon) and fallow deer (Dama dama) as well as additional data from 26 Danish red deer close to the German border and four zoo species not native to Germany. No PRNP sequence variation was observed in roe and fallow deer, as previously described for populations across Europe. In contrast, a broad PRNP variation was detected in red deer, with non-synonymous polymorphisms at codons 98, 226 and 247 as well as synonymous mutations at codons 21, 78, 136 and 185. Moreover, a novel 24 bp deletion within the octapeptide repeat was detected. In summary, 14 genotypes were seen in red deer with significant differences in their geographical distribution and frequencies, including geographical clustering of certain genotypes, suggesting “PRNP-linages” in this species. Based on data from North American CWD and the genotyping results of the European CWD cases, we would predict that large proportions of wild cervids in Europe might be susceptible to CWD once introduced to naive populations. |
format |
Article in Journal/Newspaper |
author |
Sonja Ernst Agata Piestrzyńska-Kajtoch Jörn Gethmann Małgorzata Natonek-Wiśniewska Balal Sadeghi Miroslaw P. Polak Markus Keller Dolores Gavier-Widén Katayoun Moazami-Goudarzi Fiona Houston Martin H. Groschup Christine Fast |
author_facet |
Sonja Ernst Agata Piestrzyńska-Kajtoch Jörn Gethmann Małgorzata Natonek-Wiśniewska Balal Sadeghi Miroslaw P. Polak Markus Keller Dolores Gavier-Widén Katayoun Moazami-Goudarzi Fiona Houston Martin H. Groschup Christine Fast |
author_sort |
Sonja Ernst |
title |
Prion protein gene (PRNP) variation in German and Danish cervids |
title_short |
Prion protein gene (PRNP) variation in German and Danish cervids |
title_full |
Prion protein gene (PRNP) variation in German and Danish cervids |
title_fullStr |
Prion protein gene (PRNP) variation in German and Danish cervids |
title_full_unstemmed |
Prion protein gene (PRNP) variation in German and Danish cervids |
title_sort |
prion protein gene (prnp) variation in german and danish cervids |
publisher |
BMC |
publishDate |
2024 |
url |
https://doi.org/10.1186/s13567-024-01340-8 https://doaj.org/article/e948b18743cb4d5abc36dd15412c251c |
genre |
Alces alces Rangifer tarandus |
genre_facet |
Alces alces Rangifer tarandus |
op_source |
Veterinary Research, Vol 55, Iss 1, Pp 1-17 (2024) |
op_relation |
https://doi.org/10.1186/s13567-024-01340-8 https://doaj.org/toc/1297-9716 doi:10.1186/s13567-024-01340-8 1297-9716 https://doaj.org/article/e948b18743cb4d5abc36dd15412c251c |
op_doi |
https://doi.org/10.1186/s13567-024-01340-8 |
container_title |
Veterinary Research |
container_volume |
55 |
container_issue |
1 |
_version_ |
1809817878458793984 |