Implementation of a study to examine the persistence of Ebola virus in the body fluids of Ebola virus disease survivors in Sierra Leone: Methodology and lessons learned.
BACKGROUND:The 2013-2016 West African Ebola virus disease epidemic was unprecedented in terms of the number of cases and survivors. Prior to this epidemic there was limited data available on the persistence of Ebola virus in survivors' body fluids and the potential risk of transmission, includi...
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ftdoajarticles:oai:doaj.org/article:e93b2829903046f2abe94a83458b9097 2023-05-15T15:14:40+02:00 Implementation of a study to examine the persistence of Ebola virus in the body fluids of Ebola virus disease survivors in Sierra Leone: Methodology and lessons learned. Gibrilla Fadlu Deen Suzanna L R McDonald Jaclyn E Marrinan Foday R Sesay Elizabeth Ervin Anna E Thorson Wenbo Xu Ute Ströher Patricia Ongpin Neetu Abad Archchun Ariyarajah Tasneem Malik Hongtu Liu Christine Ross Kara N Durski Philippe Gaillard Oliver Morgan Pierre Formenty Barbara Knust Nathalie Broutet Foday Sahr Sierra Leone Ebola Virus Persistence Study Group 2017-09-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0005723 https://doaj.org/article/e93b2829903046f2abe94a83458b9097 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5593174?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005723 https://doaj.org/article/e93b2829903046f2abe94a83458b9097 PLoS Neglected Tropical Diseases, Vol 11, Iss 9, p e0005723 (2017) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2017 ftdoajarticles https://doi.org/10.1371/journal.pntd.0005723 2022-12-31T09:35:55Z BACKGROUND:The 2013-2016 West African Ebola virus disease epidemic was unprecedented in terms of the number of cases and survivors. Prior to this epidemic there was limited data available on the persistence of Ebola virus in survivors' body fluids and the potential risk of transmission, including sexual transmission. METHODOLOGY/PRINCIPAL FINDINGS:Given the urgent need to determine the persistence of Ebola virus in survivors' body fluids, an observational cohort study was designed and implemented during the epidemic response operation in Sierra Leone. This publication describes study implementation methodology and the key lessons learned. Challenges encountered during implementation included unforeseen duration of follow-up, complexity of interpreting and communicating laboratory results to survivors, and the urgency of translating research findings into public health practice. Strong community engagement helped rapidly implement the study during the epidemic. The study was conducted in two phases. The first phase was initiated within five months of initial protocol discussions and assessed persistence of Ebola virus in semen of 100 adult men. The second phase assessed the persistence of virus in multiple body fluids (semen or vaginal fluid, menstrual blood, breast milk, and urine, rectal fluid, sweat, saliva, tears), of 120 men and 120 women. CONCLUSION/SIGNIFICANCE:Data from this study informed national and global guidelines in real time and demonstrated the need to implement semen testing programs among Ebola virus disease survivors. The lessons learned and study tools developed accelerated the implementation of such programs in Ebola virus disease affected countries, and also informed studies examining persistence of Zika virus. Research is a vital component of the public health response to an epidemic of a poorly characterized disease. Adequate resources should be rapidly made available to answer critical research questions, in order to better inform response efforts. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 11 9 e0005723 |
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Directory of Open Access Journals: DOAJ Articles |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Gibrilla Fadlu Deen Suzanna L R McDonald Jaclyn E Marrinan Foday R Sesay Elizabeth Ervin Anna E Thorson Wenbo Xu Ute Ströher Patricia Ongpin Neetu Abad Archchun Ariyarajah Tasneem Malik Hongtu Liu Christine Ross Kara N Durski Philippe Gaillard Oliver Morgan Pierre Formenty Barbara Knust Nathalie Broutet Foday Sahr Sierra Leone Ebola Virus Persistence Study Group Implementation of a study to examine the persistence of Ebola virus in the body fluids of Ebola virus disease survivors in Sierra Leone: Methodology and lessons learned. |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND:The 2013-2016 West African Ebola virus disease epidemic was unprecedented in terms of the number of cases and survivors. Prior to this epidemic there was limited data available on the persistence of Ebola virus in survivors' body fluids and the potential risk of transmission, including sexual transmission. METHODOLOGY/PRINCIPAL FINDINGS:Given the urgent need to determine the persistence of Ebola virus in survivors' body fluids, an observational cohort study was designed and implemented during the epidemic response operation in Sierra Leone. This publication describes study implementation methodology and the key lessons learned. Challenges encountered during implementation included unforeseen duration of follow-up, complexity of interpreting and communicating laboratory results to survivors, and the urgency of translating research findings into public health practice. Strong community engagement helped rapidly implement the study during the epidemic. The study was conducted in two phases. The first phase was initiated within five months of initial protocol discussions and assessed persistence of Ebola virus in semen of 100 adult men. The second phase assessed the persistence of virus in multiple body fluids (semen or vaginal fluid, menstrual blood, breast milk, and urine, rectal fluid, sweat, saliva, tears), of 120 men and 120 women. CONCLUSION/SIGNIFICANCE:Data from this study informed national and global guidelines in real time and demonstrated the need to implement semen testing programs among Ebola virus disease survivors. The lessons learned and study tools developed accelerated the implementation of such programs in Ebola virus disease affected countries, and also informed studies examining persistence of Zika virus. Research is a vital component of the public health response to an epidemic of a poorly characterized disease. Adequate resources should be rapidly made available to answer critical research questions, in order to better inform response efforts. |
format |
Article in Journal/Newspaper |
author |
Gibrilla Fadlu Deen Suzanna L R McDonald Jaclyn E Marrinan Foday R Sesay Elizabeth Ervin Anna E Thorson Wenbo Xu Ute Ströher Patricia Ongpin Neetu Abad Archchun Ariyarajah Tasneem Malik Hongtu Liu Christine Ross Kara N Durski Philippe Gaillard Oliver Morgan Pierre Formenty Barbara Knust Nathalie Broutet Foday Sahr Sierra Leone Ebola Virus Persistence Study Group |
author_facet |
Gibrilla Fadlu Deen Suzanna L R McDonald Jaclyn E Marrinan Foday R Sesay Elizabeth Ervin Anna E Thorson Wenbo Xu Ute Ströher Patricia Ongpin Neetu Abad Archchun Ariyarajah Tasneem Malik Hongtu Liu Christine Ross Kara N Durski Philippe Gaillard Oliver Morgan Pierre Formenty Barbara Knust Nathalie Broutet Foday Sahr Sierra Leone Ebola Virus Persistence Study Group |
author_sort |
Gibrilla Fadlu Deen |
title |
Implementation of a study to examine the persistence of Ebola virus in the body fluids of Ebola virus disease survivors in Sierra Leone: Methodology and lessons learned. |
title_short |
Implementation of a study to examine the persistence of Ebola virus in the body fluids of Ebola virus disease survivors in Sierra Leone: Methodology and lessons learned. |
title_full |
Implementation of a study to examine the persistence of Ebola virus in the body fluids of Ebola virus disease survivors in Sierra Leone: Methodology and lessons learned. |
title_fullStr |
Implementation of a study to examine the persistence of Ebola virus in the body fluids of Ebola virus disease survivors in Sierra Leone: Methodology and lessons learned. |
title_full_unstemmed |
Implementation of a study to examine the persistence of Ebola virus in the body fluids of Ebola virus disease survivors in Sierra Leone: Methodology and lessons learned. |
title_sort |
implementation of a study to examine the persistence of ebola virus in the body fluids of ebola virus disease survivors in sierra leone: methodology and lessons learned. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2017 |
url |
https://doi.org/10.1371/journal.pntd.0005723 https://doaj.org/article/e93b2829903046f2abe94a83458b9097 |
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Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 11, Iss 9, p e0005723 (2017) |
op_relation |
http://europepmc.org/articles/PMC5593174?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005723 https://doaj.org/article/e93b2829903046f2abe94a83458b9097 |
op_doi |
https://doi.org/10.1371/journal.pntd.0005723 |
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PLOS Neglected Tropical Diseases |
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11 |
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9 |
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e0005723 |
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