Strong in combination: Polyphasic approach enhances arguments for cold‐assigned cyanobacterial endemism
Abstract Cyanobacteria of biological soil crusts (BSCs) represent an important part of circumpolar and Alpine ecosystems, serve as indicators for ecological condition and climate change, and function as ecosystem engineers by soil stabilization or carbon and nitrogen input. The characterization of c...
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ftdoajarticles:oai:doaj.org/article:e927ddea0f674398ad6931c4965317e4 2023-05-15T13:46:45+02:00 Strong in combination: Polyphasic approach enhances arguments for cold‐assigned cyanobacterial endemism Patrick Jung Laura Briegel‐Williams Michael Schermer Burkhard Büdel 2019-05-01T00:00:00Z https://doi.org/10.1002/mbo3.729 https://doaj.org/article/e927ddea0f674398ad6931c4965317e4 EN eng Wiley https://doi.org/10.1002/mbo3.729 https://doaj.org/toc/2045-8827 2045-8827 doi:10.1002/mbo3.729 https://doaj.org/article/e927ddea0f674398ad6931c4965317e4 MicrobiologyOpen, Vol 8, Iss 5, Pp n/a-n/a (2019) Antarctica Arctic biogeography biological soil crusts cyanobacteria denaturing gradient gel electrophoresis Microbiology QR1-502 article 2019 ftdoajarticles https://doi.org/10.1002/mbo3.729 2022-12-31T01:55:36Z Abstract Cyanobacteria of biological soil crusts (BSCs) represent an important part of circumpolar and Alpine ecosystems, serve as indicators for ecological condition and climate change, and function as ecosystem engineers by soil stabilization or carbon and nitrogen input. The characterization of cyanobacteria from both polar regions remains extremely important to understand geographic distribution patterns and community compositions. This study is the first of its kind revealing the efficiency of combining denaturing gradient gel electrophoresis (DGGE), light microscopy and culture‐based 16S rRNA gene sequencing, applied to polar and Alpine cyanobacteria dominated BSCs. This study aimed to show the living proportion of cyanobacteria as an extension to previously published meta‐transcriptome data of the same study sites. Molecular fingerprints showed a distinct clustering of cyanobacterial communities with a close relationship between Arctic and Alpine populations, which differed from those found in Antarctica. Species richness and diversity supported these results, which were also confirmed by microscopic investigations of living cyanobacteria from the BSCs. Isolate‐based sequencing corroborated these trends as cold biome clades were assigned, which included a potentially new Arctic clade of Oculatella. Thus, our results contribute to the debate regarding biogeography of cyanobacteria of cold biomes. Article in Journal/Newspaper Antarc* Antarctica Arctic Climate change Directory of Open Access Journals: DOAJ Articles Arctic MicrobiologyOpen 8 5 e00729 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Antarctica Arctic biogeography biological soil crusts cyanobacteria denaturing gradient gel electrophoresis Microbiology QR1-502 |
spellingShingle |
Antarctica Arctic biogeography biological soil crusts cyanobacteria denaturing gradient gel electrophoresis Microbiology QR1-502 Patrick Jung Laura Briegel‐Williams Michael Schermer Burkhard Büdel Strong in combination: Polyphasic approach enhances arguments for cold‐assigned cyanobacterial endemism |
topic_facet |
Antarctica Arctic biogeography biological soil crusts cyanobacteria denaturing gradient gel electrophoresis Microbiology QR1-502 |
description |
Abstract Cyanobacteria of biological soil crusts (BSCs) represent an important part of circumpolar and Alpine ecosystems, serve as indicators for ecological condition and climate change, and function as ecosystem engineers by soil stabilization or carbon and nitrogen input. The characterization of cyanobacteria from both polar regions remains extremely important to understand geographic distribution patterns and community compositions. This study is the first of its kind revealing the efficiency of combining denaturing gradient gel electrophoresis (DGGE), light microscopy and culture‐based 16S rRNA gene sequencing, applied to polar and Alpine cyanobacteria dominated BSCs. This study aimed to show the living proportion of cyanobacteria as an extension to previously published meta‐transcriptome data of the same study sites. Molecular fingerprints showed a distinct clustering of cyanobacterial communities with a close relationship between Arctic and Alpine populations, which differed from those found in Antarctica. Species richness and diversity supported these results, which were also confirmed by microscopic investigations of living cyanobacteria from the BSCs. Isolate‐based sequencing corroborated these trends as cold biome clades were assigned, which included a potentially new Arctic clade of Oculatella. Thus, our results contribute to the debate regarding biogeography of cyanobacteria of cold biomes. |
format |
Article in Journal/Newspaper |
author |
Patrick Jung Laura Briegel‐Williams Michael Schermer Burkhard Büdel |
author_facet |
Patrick Jung Laura Briegel‐Williams Michael Schermer Burkhard Büdel |
author_sort |
Patrick Jung |
title |
Strong in combination: Polyphasic approach enhances arguments for cold‐assigned cyanobacterial endemism |
title_short |
Strong in combination: Polyphasic approach enhances arguments for cold‐assigned cyanobacterial endemism |
title_full |
Strong in combination: Polyphasic approach enhances arguments for cold‐assigned cyanobacterial endemism |
title_fullStr |
Strong in combination: Polyphasic approach enhances arguments for cold‐assigned cyanobacterial endemism |
title_full_unstemmed |
Strong in combination: Polyphasic approach enhances arguments for cold‐assigned cyanobacterial endemism |
title_sort |
strong in combination: polyphasic approach enhances arguments for cold‐assigned cyanobacterial endemism |
publisher |
Wiley |
publishDate |
2019 |
url |
https://doi.org/10.1002/mbo3.729 https://doaj.org/article/e927ddea0f674398ad6931c4965317e4 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Antarc* Antarctica Arctic Climate change |
genre_facet |
Antarc* Antarctica Arctic Climate change |
op_source |
MicrobiologyOpen, Vol 8, Iss 5, Pp n/a-n/a (2019) |
op_relation |
https://doi.org/10.1002/mbo3.729 https://doaj.org/toc/2045-8827 2045-8827 doi:10.1002/mbo3.729 https://doaj.org/article/e927ddea0f674398ad6931c4965317e4 |
op_doi |
https://doi.org/10.1002/mbo3.729 |
container_title |
MicrobiologyOpen |
container_volume |
8 |
container_issue |
5 |
container_start_page |
e00729 |
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1766245169461460992 |