Enhanced cytotoxic effect on human lung carcinoma cell line (A549) by gold nanoparticles synthesized from Justicia adhatoda leaf extract
Objective: To synthesize bio-inspired gold nanoparticles (AuNPs) using the leaf extract of Justicia adhatoda and evaluate the anti-cancer activity on human lung cancer cell line (A549). Methods: Synthesis of AuNPs was done using an aqueous leaf extract of Justicia adhatoda as a green route. The bio-...
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ftdoajarticles:oai:doaj.org/article:e8a9601de79a4d6ea6fa396760137689 2023-05-15T15:13:16+02:00 Enhanced cytotoxic effect on human lung carcinoma cell line (A549) by gold nanoparticles synthesized from Justicia adhatoda leaf extract D Latha P Prabu C Arulvasu R Manikandan S Sampurnam V Narayanan 2018-01-01T00:00:00Z https://doi.org/10.4103/2221-1691.245969 https://doaj.org/article/e8a9601de79a4d6ea6fa396760137689 EN eng Wolters Kluwer Medknow Publications http://www.apjtb.org/article.asp?issn=2221-1691;year=2018;volume=8;issue=11;spage=540;epage=547;aulast=Latha https://doaj.org/toc/2221-1691 2221-1691 doi:10.4103/2221-1691.245969 https://doaj.org/article/e8a9601de79a4d6ea6fa396760137689 Asian Pacific Journal of Tropical Biomedicine, Vol 8, Iss 11, Pp 540-547 (2018) justicia adhatoda leaf extract aunps cytotoxicity a549 cell line Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2018 ftdoajarticles https://doi.org/10.4103/2221-1691.245969 2022-12-31T04:16:53Z Objective: To synthesize bio-inspired gold nanoparticles (AuNPs) using the leaf extract of Justicia adhatoda and evaluate the anti-cancer activity on human lung cancer cell line (A549). Methods: Synthesis of AuNPs was done using an aqueous leaf extract of Justicia adhatoda as a green route. The bio-synthesized AuNPs were confirmed and characterized by using various spectral studies such as UV-Vis spectrum, Scanning Electron Microscope with EDAX, Transmission Electron Microscope, Fourier Transmission Infrared Spectroscope analysis and Surface Enhanced Raman Spectroscopy. The cell viability was determined by MTT reduction assay. In addition, cytomorphology and the nuclear morphological study of A549 cell line was observed under fluorescence microscope. Results: UV-Vis spectrum showed surface plasmon resonance peak at 547 nm, scanning electron microscope and transmission electron microscope studies showed the monodispersed spherical shape and its average size in the range of 40.1 nm was noticed. Fourier Transmission Infrared Spectroscope analysis confirmed that the C=O group of amino acids of proteins had strong ability to bind with the surface of nanoparticle. Interestingly, our results also demonstrated inhibited proliferation of A549 cell line by MTT (IC50 value: 80 μg/mL). Cell morphology was observed and cell death was caused by apoptosis as revealed by propidium iodide staining. Conclusions: The current study proves the anticancer potential of bio-synthesized AuNPs. Thus, synthesized AuNPs can be used for the treatment of human lung cancer cell (A549) and it can be exploited for drug delivery in future. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 8 11 540 |
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justicia adhatoda leaf extract aunps cytotoxicity a549 cell line Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
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justicia adhatoda leaf extract aunps cytotoxicity a549 cell line Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 D Latha P Prabu C Arulvasu R Manikandan S Sampurnam V Narayanan Enhanced cytotoxic effect on human lung carcinoma cell line (A549) by gold nanoparticles synthesized from Justicia adhatoda leaf extract |
topic_facet |
justicia adhatoda leaf extract aunps cytotoxicity a549 cell line Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To synthesize bio-inspired gold nanoparticles (AuNPs) using the leaf extract of Justicia adhatoda and evaluate the anti-cancer activity on human lung cancer cell line (A549). Methods: Synthesis of AuNPs was done using an aqueous leaf extract of Justicia adhatoda as a green route. The bio-synthesized AuNPs were confirmed and characterized by using various spectral studies such as UV-Vis spectrum, Scanning Electron Microscope with EDAX, Transmission Electron Microscope, Fourier Transmission Infrared Spectroscope analysis and Surface Enhanced Raman Spectroscopy. The cell viability was determined by MTT reduction assay. In addition, cytomorphology and the nuclear morphological study of A549 cell line was observed under fluorescence microscope. Results: UV-Vis spectrum showed surface plasmon resonance peak at 547 nm, scanning electron microscope and transmission electron microscope studies showed the monodispersed spherical shape and its average size in the range of 40.1 nm was noticed. Fourier Transmission Infrared Spectroscope analysis confirmed that the C=O group of amino acids of proteins had strong ability to bind with the surface of nanoparticle. Interestingly, our results also demonstrated inhibited proliferation of A549 cell line by MTT (IC50 value: 80 μg/mL). Cell morphology was observed and cell death was caused by apoptosis as revealed by propidium iodide staining. Conclusions: The current study proves the anticancer potential of bio-synthesized AuNPs. Thus, synthesized AuNPs can be used for the treatment of human lung cancer cell (A549) and it can be exploited for drug delivery in future. |
format |
Article in Journal/Newspaper |
author |
D Latha P Prabu C Arulvasu R Manikandan S Sampurnam V Narayanan |
author_facet |
D Latha P Prabu C Arulvasu R Manikandan S Sampurnam V Narayanan |
author_sort |
D Latha |
title |
Enhanced cytotoxic effect on human lung carcinoma cell line (A549) by gold nanoparticles synthesized from Justicia adhatoda leaf extract |
title_short |
Enhanced cytotoxic effect on human lung carcinoma cell line (A549) by gold nanoparticles synthesized from Justicia adhatoda leaf extract |
title_full |
Enhanced cytotoxic effect on human lung carcinoma cell line (A549) by gold nanoparticles synthesized from Justicia adhatoda leaf extract |
title_fullStr |
Enhanced cytotoxic effect on human lung carcinoma cell line (A549) by gold nanoparticles synthesized from Justicia adhatoda leaf extract |
title_full_unstemmed |
Enhanced cytotoxic effect on human lung carcinoma cell line (A549) by gold nanoparticles synthesized from Justicia adhatoda leaf extract |
title_sort |
enhanced cytotoxic effect on human lung carcinoma cell line (a549) by gold nanoparticles synthesized from justicia adhatoda leaf extract |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2018 |
url |
https://doi.org/10.4103/2221-1691.245969 https://doaj.org/article/e8a9601de79a4d6ea6fa396760137689 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 8, Iss 11, Pp 540-547 (2018) |
op_relation |
http://www.apjtb.org/article.asp?issn=2221-1691;year=2018;volume=8;issue=11;spage=540;epage=547;aulast=Latha https://doaj.org/toc/2221-1691 2221-1691 doi:10.4103/2221-1691.245969 https://doaj.org/article/e8a9601de79a4d6ea6fa396760137689 |
op_doi |
https://doi.org/10.4103/2221-1691.245969 |
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Asian Pacific Journal of Tropical Biomedicine |
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8 |
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11 |
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540 |
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