HSP superfamily of genes in the malaria vector Anopheles sinensis: diversity, phylogenetics and association with pyrethroid resistance
Abstract Background Heat shock proteins (HSPs) are molecular chaperones that are involved in many normal cellular processes and various kinds of environmental stress. There is still no report regarding the diversity and phylogenetics research of HSP superfamily of genes at whole genome level in inse...
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ftdoajarticles:oai:doaj.org/article:e872da2d686e4f449e748bc31255630a 2023-05-15T15:15:07+02:00 HSP superfamily of genes in the malaria vector Anopheles sinensis: diversity, phylogenetics and association with pyrethroid resistance Feng-Ling Si Liang Qiao Qi-Yi He Yong Zhou Zhen-Tian Yan Bin Chen 2019-04-01T00:00:00Z https://doi.org/10.1186/s12936-019-2770-6 https://doaj.org/article/e872da2d686e4f449e748bc31255630a EN eng BMC http://link.springer.com/article/10.1186/s12936-019-2770-6 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-019-2770-6 1475-2875 https://doaj.org/article/e872da2d686e4f449e748bc31255630a Malaria Journal, Vol 18, Iss 1, Pp 1-15 (2019) Anopheles sinensis Heat shock protein (HSP) Genome-wide identification Characteristics Expression profile Pyrethroid resistance Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2019 ftdoajarticles https://doi.org/10.1186/s12936-019-2770-6 2022-12-31T00:35:52Z Abstract Background Heat shock proteins (HSPs) are molecular chaperones that are involved in many normal cellular processes and various kinds of environmental stress. There is still no report regarding the diversity and phylogenetics research of HSP superfamily of genes at whole genome level in insects, and the HSP gene association with pyrethroid resistance is also not well known. The present study investigated the diversity, classification, scaffold location, characteristics, and phylogenetics of the superfamily of genes in Anopheles sinensis genome, and the HSP genes associated with pyrethroid resistance. Methods The present study identified the HSP genes in the An. sinensis genome, analysed their characteristics, and deduced phylogenetic relationships of all HSPs in An. sinensis, Anopheles gambiae, Culex quinquefasciatus and Aedes aegypti by bioinformatic methods. Importantly, the present study screened the HSPs associated with pyrethroid resistance using three field pyrethroid-resistant populations with RNA-seq and RT-qPCR, and looked over the HSP gene expression pattern for the first time in An. sinensis on the time-scale post insecticide treatment with RT-qPCR. Results There are 72 HSP genes in An. sinensis genome, and they are classified into five families and 11 subfamilies based on their molecular weight, homology and phylogenetics. Both RNA-seq and qPCR analysis revealed that the expression of AsHSP90AB, AsHSP70-2 and AsHSP21.7 are significantly upregulated in at least one field pyrethroid-resistant population. Eleven genes are significantly upregulated in different period after pyrethroid exposure. The HSP90, sHSP and HSP70 families are proposed to be involved in pyrethroid stress response based in expression analyses of three field pyrethroid-resistant populations, and expression pattern on the time scale post insecticide treatment. The AsHSP90AB gene is proposed to be the essential HSP gene for pyrethroid stress response in An. sinensis. Conclusions This study provides the information frame for HSP ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 18 1 |
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English |
topic |
Anopheles sinensis Heat shock protein (HSP) Genome-wide identification Characteristics Expression profile Pyrethroid resistance Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Anopheles sinensis Heat shock protein (HSP) Genome-wide identification Characteristics Expression profile Pyrethroid resistance Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Feng-Ling Si Liang Qiao Qi-Yi He Yong Zhou Zhen-Tian Yan Bin Chen HSP superfamily of genes in the malaria vector Anopheles sinensis: diversity, phylogenetics and association with pyrethroid resistance |
topic_facet |
Anopheles sinensis Heat shock protein (HSP) Genome-wide identification Characteristics Expression profile Pyrethroid resistance Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Heat shock proteins (HSPs) are molecular chaperones that are involved in many normal cellular processes and various kinds of environmental stress. There is still no report regarding the diversity and phylogenetics research of HSP superfamily of genes at whole genome level in insects, and the HSP gene association with pyrethroid resistance is also not well known. The present study investigated the diversity, classification, scaffold location, characteristics, and phylogenetics of the superfamily of genes in Anopheles sinensis genome, and the HSP genes associated with pyrethroid resistance. Methods The present study identified the HSP genes in the An. sinensis genome, analysed their characteristics, and deduced phylogenetic relationships of all HSPs in An. sinensis, Anopheles gambiae, Culex quinquefasciatus and Aedes aegypti by bioinformatic methods. Importantly, the present study screened the HSPs associated with pyrethroid resistance using three field pyrethroid-resistant populations with RNA-seq and RT-qPCR, and looked over the HSP gene expression pattern for the first time in An. sinensis on the time-scale post insecticide treatment with RT-qPCR. Results There are 72 HSP genes in An. sinensis genome, and they are classified into five families and 11 subfamilies based on their molecular weight, homology and phylogenetics. Both RNA-seq and qPCR analysis revealed that the expression of AsHSP90AB, AsHSP70-2 and AsHSP21.7 are significantly upregulated in at least one field pyrethroid-resistant population. Eleven genes are significantly upregulated in different period after pyrethroid exposure. The HSP90, sHSP and HSP70 families are proposed to be involved in pyrethroid stress response based in expression analyses of three field pyrethroid-resistant populations, and expression pattern on the time scale post insecticide treatment. The AsHSP90AB gene is proposed to be the essential HSP gene for pyrethroid stress response in An. sinensis. Conclusions This study provides the information frame for HSP ... |
format |
Article in Journal/Newspaper |
author |
Feng-Ling Si Liang Qiao Qi-Yi He Yong Zhou Zhen-Tian Yan Bin Chen |
author_facet |
Feng-Ling Si Liang Qiao Qi-Yi He Yong Zhou Zhen-Tian Yan Bin Chen |
author_sort |
Feng-Ling Si |
title |
HSP superfamily of genes in the malaria vector Anopheles sinensis: diversity, phylogenetics and association with pyrethroid resistance |
title_short |
HSP superfamily of genes in the malaria vector Anopheles sinensis: diversity, phylogenetics and association with pyrethroid resistance |
title_full |
HSP superfamily of genes in the malaria vector Anopheles sinensis: diversity, phylogenetics and association with pyrethroid resistance |
title_fullStr |
HSP superfamily of genes in the malaria vector Anopheles sinensis: diversity, phylogenetics and association with pyrethroid resistance |
title_full_unstemmed |
HSP superfamily of genes in the malaria vector Anopheles sinensis: diversity, phylogenetics and association with pyrethroid resistance |
title_sort |
hsp superfamily of genes in the malaria vector anopheles sinensis: diversity, phylogenetics and association with pyrethroid resistance |
publisher |
BMC |
publishDate |
2019 |
url |
https://doi.org/10.1186/s12936-019-2770-6 https://doaj.org/article/e872da2d686e4f449e748bc31255630a |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 18, Iss 1, Pp 1-15 (2019) |
op_relation |
http://link.springer.com/article/10.1186/s12936-019-2770-6 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-019-2770-6 1475-2875 https://doaj.org/article/e872da2d686e4f449e748bc31255630a |
op_doi |
https://doi.org/10.1186/s12936-019-2770-6 |
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Malaria Journal |
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18 |
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1766345504985186304 |