A direct comparison of real time PCR on plasma and blood to detect Plasmodium falciparum infection in children
Abstract Background Estimation of Plasmodium falciparum parasitaemia can vary with the method used and time of sampling. Quantitative real time PCR (qPCR) on whole blood or plasma samples has previously been shown to be more sensitive than thick film microscopy. However the efficiencies of each meth...
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ftdoajarticles:oai:doaj.org/article:e83c3d9eca7d4fa996b61c081aa08471 2023-05-15T15:14:57+02:00 A direct comparison of real time PCR on plasma and blood to detect Plasmodium falciparum infection in children Lamikanra Abigail A Dobaño Carlota Jiménez Alfons Nhabomba Augusto Tsang Hoi P Guinovart Caterina Manaca Maria N Quinto Llorenç Aguilar Ruth Cisteró Pau Alonso Pedro L Roberts David J Mayor Alfredo 2012-06-01T00:00:00Z https://doi.org/10.1186/1475-2875-11-201 https://doaj.org/article/e83c3d9eca7d4fa996b61c081aa08471 EN eng BMC http://www.malariajournal.com/content/11/1/201 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-11-201 1475-2875 https://doaj.org/article/e83c3d9eca7d4fa996b61c081aa08471 Malaria Journal, Vol 11, Iss 1, p 201 (2012) Malaria qPCR Plasma Anaemia Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2012 ftdoajarticles https://doi.org/10.1186/1475-2875-11-201 2022-12-30T21:39:13Z Abstract Background Estimation of Plasmodium falciparum parasitaemia can vary with the method used and time of sampling. Quantitative real time PCR (qPCR) on whole blood or plasma samples has previously been shown to be more sensitive than thick film microscopy. However the efficiencies of each method have not been compared using samples obtained from infants less than one year old. Methods A multiple of statistical approaches were used to compare the performance of qPCR on whole blood or plasma to detect the 18 S ribosomal gene of P. falciparum in 548 samples from children aged 2.5 or 24 months. Parasite prevalence in matched samples was compared using Mcnemar’s test and agreement of positive results quantified as Kappa scores. Parasite prevalences between different age groups were compared by Fisher’s test. Results from analyses by thick film microscopy were also available from children at 24 months and their correlation to each qPCR method examined by the Spearman’s test. Finally the association of P. falciparum infection with the incidence of multiple malaria episodes from contact to 24 months of age was evaluated using negative binomial regression. Results These analyses showed that qPCR from whole blood detected approximately 3-fold more cases of infection than plasma qPCR. Both qPCR methods agreed well with each other although qPCR from plasma had a greater agreement with microscopy (96.85%) than did qPCR from blood (69.7%). At 24 months the prevalence of infection detected by all methods was associated with anaemia (p < 0.05). Conclusions The data presented here demonstrates that low levels of parasitaemia are better detected by qPCR using parasite DNA from whole blood than from plasma. However plasma samples provide a viable substitute when parasite smears are unavailable. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 11 1 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Malaria qPCR Plasma Anaemia Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Malaria qPCR Plasma Anaemia Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Lamikanra Abigail A Dobaño Carlota Jiménez Alfons Nhabomba Augusto Tsang Hoi P Guinovart Caterina Manaca Maria N Quinto Llorenç Aguilar Ruth Cisteró Pau Alonso Pedro L Roberts David J Mayor Alfredo A direct comparison of real time PCR on plasma and blood to detect Plasmodium falciparum infection in children |
topic_facet |
Malaria qPCR Plasma Anaemia Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Estimation of Plasmodium falciparum parasitaemia can vary with the method used and time of sampling. Quantitative real time PCR (qPCR) on whole blood or plasma samples has previously been shown to be more sensitive than thick film microscopy. However the efficiencies of each method have not been compared using samples obtained from infants less than one year old. Methods A multiple of statistical approaches were used to compare the performance of qPCR on whole blood or plasma to detect the 18 S ribosomal gene of P. falciparum in 548 samples from children aged 2.5 or 24 months. Parasite prevalence in matched samples was compared using Mcnemar’s test and agreement of positive results quantified as Kappa scores. Parasite prevalences between different age groups were compared by Fisher’s test. Results from analyses by thick film microscopy were also available from children at 24 months and their correlation to each qPCR method examined by the Spearman’s test. Finally the association of P. falciparum infection with the incidence of multiple malaria episodes from contact to 24 months of age was evaluated using negative binomial regression. Results These analyses showed that qPCR from whole blood detected approximately 3-fold more cases of infection than plasma qPCR. Both qPCR methods agreed well with each other although qPCR from plasma had a greater agreement with microscopy (96.85%) than did qPCR from blood (69.7%). At 24 months the prevalence of infection detected by all methods was associated with anaemia (p < 0.05). Conclusions The data presented here demonstrates that low levels of parasitaemia are better detected by qPCR using parasite DNA from whole blood than from plasma. However plasma samples provide a viable substitute when parasite smears are unavailable. |
format |
Article in Journal/Newspaper |
author |
Lamikanra Abigail A Dobaño Carlota Jiménez Alfons Nhabomba Augusto Tsang Hoi P Guinovart Caterina Manaca Maria N Quinto Llorenç Aguilar Ruth Cisteró Pau Alonso Pedro L Roberts David J Mayor Alfredo |
author_facet |
Lamikanra Abigail A Dobaño Carlota Jiménez Alfons Nhabomba Augusto Tsang Hoi P Guinovart Caterina Manaca Maria N Quinto Llorenç Aguilar Ruth Cisteró Pau Alonso Pedro L Roberts David J Mayor Alfredo |
author_sort |
Lamikanra Abigail A |
title |
A direct comparison of real time PCR on plasma and blood to detect Plasmodium falciparum infection in children |
title_short |
A direct comparison of real time PCR on plasma and blood to detect Plasmodium falciparum infection in children |
title_full |
A direct comparison of real time PCR on plasma and blood to detect Plasmodium falciparum infection in children |
title_fullStr |
A direct comparison of real time PCR on plasma and blood to detect Plasmodium falciparum infection in children |
title_full_unstemmed |
A direct comparison of real time PCR on plasma and blood to detect Plasmodium falciparum infection in children |
title_sort |
direct comparison of real time pcr on plasma and blood to detect plasmodium falciparum infection in children |
publisher |
BMC |
publishDate |
2012 |
url |
https://doi.org/10.1186/1475-2875-11-201 https://doaj.org/article/e83c3d9eca7d4fa996b61c081aa08471 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 11, Iss 1, p 201 (2012) |
op_relation |
http://www.malariajournal.com/content/11/1/201 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-11-201 1475-2875 https://doaj.org/article/e83c3d9eca7d4fa996b61c081aa08471 |
op_doi |
https://doi.org/10.1186/1475-2875-11-201 |
container_title |
Malaria Journal |
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11 |
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1 |
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1766345348030136320 |