Invariant NKT cell response to dengue virus infection in human.
BACKGROUND:Dengue viral infection is a global health threat without vaccine or specific treatment. The clinical outcome varies from asymptomatic, mild dengue fever (DF) to severe dengue hemorrhagic fever (DHF). While adaptive immune responses were found to be detrimental in the dengue pathogenesis,...
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ftdoajarticles:oai:doaj.org/article:e81a7836d23b493e94db7e9f53740432 2023-05-15T15:10:35+02:00 Invariant NKT cell response to dengue virus infection in human. Ponpan Matangkasombut Wilawan Chan-In Anunya Opasawaschai Pisut Pongchaikul Nattaya Tangthawornchaikul Sirijitt Vasanawathana Wannee Limpitikul Prida Malasit Thaneeya Duangchinda Gavin Screaton Juthathip Mongkolsapaya 2014-06-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0002955 https://doaj.org/article/e81a7836d23b493e94db7e9f53740432 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4063705?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0002955 https://doaj.org/article/e81a7836d23b493e94db7e9f53740432 PLoS Neglected Tropical Diseases, Vol 8, Iss 6, p e2955 (2014) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2014 ftdoajarticles https://doi.org/10.1371/journal.pntd.0002955 2022-12-31T03:00:43Z BACKGROUND:Dengue viral infection is a global health threat without vaccine or specific treatment. The clinical outcome varies from asymptomatic, mild dengue fever (DF) to severe dengue hemorrhagic fever (DHF). While adaptive immune responses were found to be detrimental in the dengue pathogenesis, the roles of earlier innate events remain largely uninvestigated. Invariant natural killer T (iNKT) cells represent innate-like T cells that could dictate subsequent adaptive response but their role in human dengue virus infection is not known. We hypothesized that iNKT cells play a role in human dengue infection. METHODS:Blood samples from a well-characterized cohort of children with DF, DHF, in comparison to non-dengue febrile illness (OFI) and healthy controls at various time points were studied. iNKT cells activation were analyzed by the expression of CD69 by flow cytometry. Their cytokine production was then analyzed after α-GalCer stimulation. Further, the CD1d expression on monocytes, and CD69 expression on conventional T cells were measured. RESULTS:iNKT cells were activated during acute dengue infection. The level of iNKT cell activation associates with the disease severity. Furthermore, these iNKT cells had altered functional response to subsequent ex vivo stimulation with α-GalCer. Moreover, during acute dengue infection, monocytic CD1d expression was also upregulated and conventional T cells also became activated. CONCLUSION:iNKT cells might play an early and critical role in the pathogenesis of severe dengue viral infection in human. Targeting iNKT cells and CD1d serve as a potential therapeutic strategy for severe dengue infection in the future. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 8 6 e2955 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Ponpan Matangkasombut Wilawan Chan-In Anunya Opasawaschai Pisut Pongchaikul Nattaya Tangthawornchaikul Sirijitt Vasanawathana Wannee Limpitikul Prida Malasit Thaneeya Duangchinda Gavin Screaton Juthathip Mongkolsapaya Invariant NKT cell response to dengue virus infection in human. |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND:Dengue viral infection is a global health threat without vaccine or specific treatment. The clinical outcome varies from asymptomatic, mild dengue fever (DF) to severe dengue hemorrhagic fever (DHF). While adaptive immune responses were found to be detrimental in the dengue pathogenesis, the roles of earlier innate events remain largely uninvestigated. Invariant natural killer T (iNKT) cells represent innate-like T cells that could dictate subsequent adaptive response but their role in human dengue virus infection is not known. We hypothesized that iNKT cells play a role in human dengue infection. METHODS:Blood samples from a well-characterized cohort of children with DF, DHF, in comparison to non-dengue febrile illness (OFI) and healthy controls at various time points were studied. iNKT cells activation were analyzed by the expression of CD69 by flow cytometry. Their cytokine production was then analyzed after α-GalCer stimulation. Further, the CD1d expression on monocytes, and CD69 expression on conventional T cells were measured. RESULTS:iNKT cells were activated during acute dengue infection. The level of iNKT cell activation associates with the disease severity. Furthermore, these iNKT cells had altered functional response to subsequent ex vivo stimulation with α-GalCer. Moreover, during acute dengue infection, monocytic CD1d expression was also upregulated and conventional T cells also became activated. CONCLUSION:iNKT cells might play an early and critical role in the pathogenesis of severe dengue viral infection in human. Targeting iNKT cells and CD1d serve as a potential therapeutic strategy for severe dengue infection in the future. |
format |
Article in Journal/Newspaper |
author |
Ponpan Matangkasombut Wilawan Chan-In Anunya Opasawaschai Pisut Pongchaikul Nattaya Tangthawornchaikul Sirijitt Vasanawathana Wannee Limpitikul Prida Malasit Thaneeya Duangchinda Gavin Screaton Juthathip Mongkolsapaya |
author_facet |
Ponpan Matangkasombut Wilawan Chan-In Anunya Opasawaschai Pisut Pongchaikul Nattaya Tangthawornchaikul Sirijitt Vasanawathana Wannee Limpitikul Prida Malasit Thaneeya Duangchinda Gavin Screaton Juthathip Mongkolsapaya |
author_sort |
Ponpan Matangkasombut |
title |
Invariant NKT cell response to dengue virus infection in human. |
title_short |
Invariant NKT cell response to dengue virus infection in human. |
title_full |
Invariant NKT cell response to dengue virus infection in human. |
title_fullStr |
Invariant NKT cell response to dengue virus infection in human. |
title_full_unstemmed |
Invariant NKT cell response to dengue virus infection in human. |
title_sort |
invariant nkt cell response to dengue virus infection in human. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2014 |
url |
https://doi.org/10.1371/journal.pntd.0002955 https://doaj.org/article/e81a7836d23b493e94db7e9f53740432 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 8, Iss 6, p e2955 (2014) |
op_relation |
http://europepmc.org/articles/PMC4063705?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0002955 https://doaj.org/article/e81a7836d23b493e94db7e9f53740432 |
op_doi |
https://doi.org/10.1371/journal.pntd.0002955 |
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PLoS Neglected Tropical Diseases |
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8 |
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6 |
container_start_page |
e2955 |
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1766341583569944576 |