RNA purification-free detection of SARS-CoV-2 using reverse transcription loop-mediated isothermal amplification (RT-LAMP)

Abstract Background Current diagnosis of SARS-CoV-2 infection relies on RNA purification prior to amplification. Typical extraction methods limit the processing speed and turnaround time for SARS-CoV-2 diagnostic testing. Methods Here, we applied reverse transcription loop-mediated isothermal amplif...

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Bibliographic Details
Published in:Tropical Medicine and Health
Main Authors: Meng Yee Lai, Jeyanthi Suppiah, Ravindran Thayan, Ilyiana Ismail, Nur Izati Mustapa, Tuan Suhaila Tuan Soh, Afifah Haji Hassan, Kalaiarasu M. Peariasamy, Yee Leng Lee, Yee Ling Lau
Format: Article in Journal/Newspaper
Language:English
Published: BMC 2022
Subjects:
COVID-19
Nasopharyngeal swab
SARS-CoV-2
RT-LAMP
Arctic medicine. Tropical medicine
RC955-962
Arctic
Online Access:https://doi.org/10.1186/s41182-021-00396-y
https://doaj.org/article/e7f525a5da18408b8d3c2acf378b36c4
Description
Summary:Abstract Background Current diagnosis of SARS-CoV-2 infection relies on RNA purification prior to amplification. Typical extraction methods limit the processing speed and turnaround time for SARS-CoV-2 diagnostic testing. Methods Here, we applied reverse transcription loop-mediated isothermal amplification directly onto human clinical swabs samples to amplify the RNA from SARS-CoV-2 swab samples after processing with chelating resin. Results By testing our method on 64 samples, we managed to develop an RT-LAMP assay with 95.9% sensitivity (95% CI 86 to 99.5%) and 100% specificity (95% CI 78.2–100%). Conclusion The entire process including sample processing can be completed in approximately 50 min. This method has promising potential to be applied as a fast, simple and inexpensive diagnostic tool for the detection of SARS-CoV-2.

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