DNA multigene characterization of Fasciola hepatica and Lymnaea neotropica and its fascioliasis transmission capacity in Uruguay, with historical correlation, human report review and infection risk analysis.
BACKGROUND:Fascioliasis is a pathogenic disease transmitted by lymnaeid snails and recently emerging in humans, in part due to effects of climate changes, anthropogenic environment modifications, import/export and movements of livestock. South America is the continent presenting more human fasciolia...
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ftdoajarticles:oai:doaj.org/article:e7e6ede29d9a4126aeb634afaa798d42 2023-05-15T15:16:27+02:00 DNA multigene characterization of Fasciola hepatica and Lymnaea neotropica and its fascioliasis transmission capacity in Uruguay, with historical correlation, human report review and infection risk analysis. María Dolores Bargues Valeria Gayo Jaime Sanchis Patricio Artigas Messaoud Khoubbane Soledad Birriel Santiago Mas-Coma 2017-02-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0005352 https://doaj.org/article/e7e6ede29d9a4126aeb634afaa798d42 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5310921?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005352 https://doaj.org/article/e7e6ede29d9a4126aeb634afaa798d42 PLoS Neglected Tropical Diseases, Vol 11, Iss 2, p e0005352 (2017) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2017 ftdoajarticles https://doi.org/10.1371/journal.pntd.0005352 2022-12-31T16:03:23Z BACKGROUND:Fascioliasis is a pathogenic disease transmitted by lymnaeid snails and recently emerging in humans, in part due to effects of climate changes, anthropogenic environment modifications, import/export and movements of livestock. South America is the continent presenting more human fascioliasis hyperendemic areas and the highest prevalences and intensities known. These scenarios appear mainly linked to altitude areas in Andean countries, whereas lowland areas of non-Andean countries, such as Uruguay, only show sporadic human cases or outbreaks. A study including DNA marker sequencing of fasciolids and lymnaeids, an experimental study of the life cycle in Uruguay, and a review of human fascioliasis in Uruguay, are performed. METHODOLOGY/PRINCIPAL FINDINGS:The characterization of Fasciola hepatica from cattle and horses of Uruguay included the complete sequences of the ribosomal DNA ITS-2 and ITS-1 and mitochondrial DNA cox1 and nad1. ITS-2, ITS-1, partial cox1 and rDNA 16S gene of mtDNA were used for lymnaeids. Results indicated that vectors belong to Lymnaea neotropica instead of to Lymnaea viator, as always reported from Uruguay. The life cycle and transmission features of F. hepatica by L. neotropica of Uruguay were studied under standardized experimental conditions to enable a comparison with the transmission capacity of F. hepatica by Galba truncatula at very high altitude in Bolivia. On this baseline, we reviewed the 95 human fascioliasis cases reported in Uruguay and analyzed the risk of human infection in front of future climate change estimations. CONCLUSIONS/SIGNIFICANCE:The correlation of fasciolid and lymnaeid haplotypes with historical data on the introduction and spread of livestock into Uruguay allowed to understand the molecular diversity detected. Although Uruguayan L. neotropica is a highly efficient vector, its transmission capacity is markedly lower than that of Bolivian G. truncatula. This allows to understand the transmission and epidemiological differences between Andean highlands ... Article in Journal/Newspaper Arctic Climate change Directory of Open Access Journals: DOAJ Articles Arctic Uruguay PLOS Neglected Tropical Diseases 11 2 e0005352 |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 María Dolores Bargues Valeria Gayo Jaime Sanchis Patricio Artigas Messaoud Khoubbane Soledad Birriel Santiago Mas-Coma DNA multigene characterization of Fasciola hepatica and Lymnaea neotropica and its fascioliasis transmission capacity in Uruguay, with historical correlation, human report review and infection risk analysis. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND:Fascioliasis is a pathogenic disease transmitted by lymnaeid snails and recently emerging in humans, in part due to effects of climate changes, anthropogenic environment modifications, import/export and movements of livestock. South America is the continent presenting more human fascioliasis hyperendemic areas and the highest prevalences and intensities known. These scenarios appear mainly linked to altitude areas in Andean countries, whereas lowland areas of non-Andean countries, such as Uruguay, only show sporadic human cases or outbreaks. A study including DNA marker sequencing of fasciolids and lymnaeids, an experimental study of the life cycle in Uruguay, and a review of human fascioliasis in Uruguay, are performed. METHODOLOGY/PRINCIPAL FINDINGS:The characterization of Fasciola hepatica from cattle and horses of Uruguay included the complete sequences of the ribosomal DNA ITS-2 and ITS-1 and mitochondrial DNA cox1 and nad1. ITS-2, ITS-1, partial cox1 and rDNA 16S gene of mtDNA were used for lymnaeids. Results indicated that vectors belong to Lymnaea neotropica instead of to Lymnaea viator, as always reported from Uruguay. The life cycle and transmission features of F. hepatica by L. neotropica of Uruguay were studied under standardized experimental conditions to enable a comparison with the transmission capacity of F. hepatica by Galba truncatula at very high altitude in Bolivia. On this baseline, we reviewed the 95 human fascioliasis cases reported in Uruguay and analyzed the risk of human infection in front of future climate change estimations. CONCLUSIONS/SIGNIFICANCE:The correlation of fasciolid and lymnaeid haplotypes with historical data on the introduction and spread of livestock into Uruguay allowed to understand the molecular diversity detected. Although Uruguayan L. neotropica is a highly efficient vector, its transmission capacity is markedly lower than that of Bolivian G. truncatula. This allows to understand the transmission and epidemiological differences between Andean highlands ... |
format |
Article in Journal/Newspaper |
author |
María Dolores Bargues Valeria Gayo Jaime Sanchis Patricio Artigas Messaoud Khoubbane Soledad Birriel Santiago Mas-Coma |
author_facet |
María Dolores Bargues Valeria Gayo Jaime Sanchis Patricio Artigas Messaoud Khoubbane Soledad Birriel Santiago Mas-Coma |
author_sort |
María Dolores Bargues |
title |
DNA multigene characterization of Fasciola hepatica and Lymnaea neotropica and its fascioliasis transmission capacity in Uruguay, with historical correlation, human report review and infection risk analysis. |
title_short |
DNA multigene characterization of Fasciola hepatica and Lymnaea neotropica and its fascioliasis transmission capacity in Uruguay, with historical correlation, human report review and infection risk analysis. |
title_full |
DNA multigene characterization of Fasciola hepatica and Lymnaea neotropica and its fascioliasis transmission capacity in Uruguay, with historical correlation, human report review and infection risk analysis. |
title_fullStr |
DNA multigene characterization of Fasciola hepatica and Lymnaea neotropica and its fascioliasis transmission capacity in Uruguay, with historical correlation, human report review and infection risk analysis. |
title_full_unstemmed |
DNA multigene characterization of Fasciola hepatica and Lymnaea neotropica and its fascioliasis transmission capacity in Uruguay, with historical correlation, human report review and infection risk analysis. |
title_sort |
dna multigene characterization of fasciola hepatica and lymnaea neotropica and its fascioliasis transmission capacity in uruguay, with historical correlation, human report review and infection risk analysis. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2017 |
url |
https://doi.org/10.1371/journal.pntd.0005352 https://doaj.org/article/e7e6ede29d9a4126aeb634afaa798d42 |
geographic |
Arctic Uruguay |
geographic_facet |
Arctic Uruguay |
genre |
Arctic Climate change |
genre_facet |
Arctic Climate change |
op_source |
PLoS Neglected Tropical Diseases, Vol 11, Iss 2, p e0005352 (2017) |
op_relation |
http://europepmc.org/articles/PMC5310921?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005352 https://doaj.org/article/e7e6ede29d9a4126aeb634afaa798d42 |
op_doi |
https://doi.org/10.1371/journal.pntd.0005352 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
11 |
container_issue |
2 |
container_start_page |
e0005352 |
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1766346754895118336 |