Spot the difference-development of a syndrome based protein microarray for specific serological detection of multiple flavivirus infections in travelers.
BACKGROUND:The family Flaviviridae, genus Flavivirus, holds many of the world's most prevalent arboviral diseases that are also considered the most important travel related arboviral infections. In most cases, flavivirus diagnosis in travelers is primarily based on serology as viremia is often...
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ftdoajarticles:oai:doaj.org/article:e7bbe00cd2024de0894c461504d336a6 2023-05-15T15:10:52+02:00 Spot the difference-development of a syndrome based protein microarray for specific serological detection of multiple flavivirus infections in travelers. Natalie B Cleton Gert-Jan Godeke Johan Reimerink Mathias F Beersma H Rogier van Doorn Leticia Franco Marco Goeijenbier Miguel A Jimenez-Clavero Barbara W Johnson Matthias Niedrig Anna Papa Vittorio Sambri Adriana Tami Zoraida I Velasco-Salas Marion P G Koopmans Chantal B E M Reusken 2015-03-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0003580 https://doaj.org/article/e7bbe00cd2024de0894c461504d336a6 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4359159?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0003580 https://doaj.org/article/e7bbe00cd2024de0894c461504d336a6 PLoS Neglected Tropical Diseases, Vol 9, Iss 3, p e0003580 (2015) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2015 ftdoajarticles https://doi.org/10.1371/journal.pntd.0003580 2022-12-31T13:05:48Z BACKGROUND:The family Flaviviridae, genus Flavivirus, holds many of the world's most prevalent arboviral diseases that are also considered the most important travel related arboviral infections. In most cases, flavivirus diagnosis in travelers is primarily based on serology as viremia is often low and typically has already been reduced to undetectable levels when symptoms set in and patients seek medical attention. Serological differentiation between flaviviruses and the false-positive results caused by vaccination and cross-reactivity among the different species, are problematic for surveillance and diagnostics of flaviviruses. Their partially overlapping geographic distribution and symptoms, combined with increase in travel, and preexisting antibodies due to flavivirus vaccinations, expand the need for rapid and reliable multiplex diagnostic tests to supplement currently used methods. GOAL:We describe the development of a multiplex serological protein microarray using recombinant NS1 proteins for detection of medically important viruses within the genus Flavivirus. Sera from clinical flavivirus patients were used for primary development of the protein microarray. RESULTS:Results show a high IgG and IgM sensitivity and specificity for individual NS1 antigens, and limited cross reactivity, even within serocomplexes. In addition, the serology based on this array allows for discrimination between infection and vaccination response for JEV vaccine, and no cross-reactivity with TBEV and YFV vaccine induced antibodies when testing for antibodies to other flaviviruses. CONCLUSION:Based on these data, multiplex NS1-based protein microarray is a promising tool for surveillance and diagnosis of flaviviruses. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 9 3 e0003580 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Natalie B Cleton Gert-Jan Godeke Johan Reimerink Mathias F Beersma H Rogier van Doorn Leticia Franco Marco Goeijenbier Miguel A Jimenez-Clavero Barbara W Johnson Matthias Niedrig Anna Papa Vittorio Sambri Adriana Tami Zoraida I Velasco-Salas Marion P G Koopmans Chantal B E M Reusken Spot the difference-development of a syndrome based protein microarray for specific serological detection of multiple flavivirus infections in travelers. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND:The family Flaviviridae, genus Flavivirus, holds many of the world's most prevalent arboviral diseases that are also considered the most important travel related arboviral infections. In most cases, flavivirus diagnosis in travelers is primarily based on serology as viremia is often low and typically has already been reduced to undetectable levels when symptoms set in and patients seek medical attention. Serological differentiation between flaviviruses and the false-positive results caused by vaccination and cross-reactivity among the different species, are problematic for surveillance and diagnostics of flaviviruses. Their partially overlapping geographic distribution and symptoms, combined with increase in travel, and preexisting antibodies due to flavivirus vaccinations, expand the need for rapid and reliable multiplex diagnostic tests to supplement currently used methods. GOAL:We describe the development of a multiplex serological protein microarray using recombinant NS1 proteins for detection of medically important viruses within the genus Flavivirus. Sera from clinical flavivirus patients were used for primary development of the protein microarray. RESULTS:Results show a high IgG and IgM sensitivity and specificity for individual NS1 antigens, and limited cross reactivity, even within serocomplexes. In addition, the serology based on this array allows for discrimination between infection and vaccination response for JEV vaccine, and no cross-reactivity with TBEV and YFV vaccine induced antibodies when testing for antibodies to other flaviviruses. CONCLUSION:Based on these data, multiplex NS1-based protein microarray is a promising tool for surveillance and diagnosis of flaviviruses. |
format |
Article in Journal/Newspaper |
author |
Natalie B Cleton Gert-Jan Godeke Johan Reimerink Mathias F Beersma H Rogier van Doorn Leticia Franco Marco Goeijenbier Miguel A Jimenez-Clavero Barbara W Johnson Matthias Niedrig Anna Papa Vittorio Sambri Adriana Tami Zoraida I Velasco-Salas Marion P G Koopmans Chantal B E M Reusken |
author_facet |
Natalie B Cleton Gert-Jan Godeke Johan Reimerink Mathias F Beersma H Rogier van Doorn Leticia Franco Marco Goeijenbier Miguel A Jimenez-Clavero Barbara W Johnson Matthias Niedrig Anna Papa Vittorio Sambri Adriana Tami Zoraida I Velasco-Salas Marion P G Koopmans Chantal B E M Reusken |
author_sort |
Natalie B Cleton |
title |
Spot the difference-development of a syndrome based protein microarray for specific serological detection of multiple flavivirus infections in travelers. |
title_short |
Spot the difference-development of a syndrome based protein microarray for specific serological detection of multiple flavivirus infections in travelers. |
title_full |
Spot the difference-development of a syndrome based protein microarray for specific serological detection of multiple flavivirus infections in travelers. |
title_fullStr |
Spot the difference-development of a syndrome based protein microarray for specific serological detection of multiple flavivirus infections in travelers. |
title_full_unstemmed |
Spot the difference-development of a syndrome based protein microarray for specific serological detection of multiple flavivirus infections in travelers. |
title_sort |
spot the difference-development of a syndrome based protein microarray for specific serological detection of multiple flavivirus infections in travelers. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2015 |
url |
https://doi.org/10.1371/journal.pntd.0003580 https://doaj.org/article/e7bbe00cd2024de0894c461504d336a6 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 9, Iss 3, p e0003580 (2015) |
op_relation |
http://europepmc.org/articles/PMC4359159?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0003580 https://doaj.org/article/e7bbe00cd2024de0894c461504d336a6 |
op_doi |
https://doi.org/10.1371/journal.pntd.0003580 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
9 |
container_issue |
3 |
container_start_page |
e0003580 |
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1766341808800923648 |