Microscopic and molecular evaluation of Strongyloides venezuelensis in an experimental life cycle using Wistar rats
Introduction: Strongyloides venezuelensis is a nematode whose natural host is rats. It is used as a model for the investigation of human strongyloidiasis caused by S. stercoralis. The latter is a neglected tropical disease in Ecuador where there are no specific plans to mitigate this parasitic illne...
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ftdoajarticles:oai:doaj.org/article:e7b3ca605daf4d56b28bde0982ecb3bf 2023-05-15T15:10:32+02:00 Microscopic and molecular evaluation of Strongyloides venezuelensis in an experimental life cycle using Wistar rats Jairo Tobar Daniel Ramos Sarmiento Diana Tayupanta Melina Rodríguez Fabián Aguilar 2021-05-01T00:00:00Z https://doi.org/10.7705/biomedica.5650 https://doaj.org/article/e7b3ca605daf4d56b28bde0982ecb3bf EN ES eng spa Instituto Nacional de Salud https://revistabiomedica.org/index.php/biomedica/article/view/5650 https://doaj.org/toc/0120-4157 0120-4157 doi:10.7705/biomedica.5650 https://doaj.org/article/e7b3ca605daf4d56b28bde0982ecb3bf Biomédica: revista del Instituto Nacional de Salud, Vol 41, Iss Supl. 1, Pp 35-46 (2021) strongyloides nematoda life cycle stages intestinal diseases parasitic wistar rats ecuador Medicine R Arctic medicine. Tropical medicine RC955-962 article 2021 ftdoajarticles https://doi.org/10.7705/biomedica.5650 2022-12-31T00:57:06Z Introduction: Strongyloides venezuelensis is a nematode whose natural host is rats. It is used as a model for the investigation of human strongyloidiasis caused by S. stercoralis. The latter is a neglected tropical disease in Ecuador where there are no specific plans to mitigate this parasitic illness. Objective: To evaluate the stages of S. venezuelensis in an experimental life cycle using Wistar rats. Materials and methods: Male Wistar rats were used to replicate the natural biological cycle of S. venezuelensis and describe its morphometric characteristics, as well as its parasitic development. Furthermore, the production of eggs per gram of feces was quantified using two diagnostic techniques and assessment of parasite load: Kato-Katz and qPCR. Results: Viable larval stages (L1, L2, L3) could be obtained up to 96 hours through fecal culture. Parthenogenetic females were established in the duodenum on the fifth day postinfection. Fertile eggs were observed in the intestinal tissue and fresh feces where the production peak occurred on the 8th. day post-infection. Unlike Kato-Katz, qPCR detected parasitic DNA on days not typically reported. Conclusions: The larval migration of S. venezuelensis within the murine host in an experimental environment was equivalent to that described in its natural biological cycle. The Kato-Katz quantitative technique showed to be quick and low-cost, but the qPCR had greater diagnostic precision. This experimental life cycle can be used as a tool for the study of strongyloidiasis or other similar nematodiasis. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Biomédica 41 Supl. 1 35 46 |
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strongyloides nematoda life cycle stages intestinal diseases parasitic wistar rats ecuador Medicine R Arctic medicine. Tropical medicine RC955-962 |
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strongyloides nematoda life cycle stages intestinal diseases parasitic wistar rats ecuador Medicine R Arctic medicine. Tropical medicine RC955-962 Jairo Tobar Daniel Ramos Sarmiento Diana Tayupanta Melina Rodríguez Fabián Aguilar Microscopic and molecular evaluation of Strongyloides venezuelensis in an experimental life cycle using Wistar rats |
topic_facet |
strongyloides nematoda life cycle stages intestinal diseases parasitic wistar rats ecuador Medicine R Arctic medicine. Tropical medicine RC955-962 |
description |
Introduction: Strongyloides venezuelensis is a nematode whose natural host is rats. It is used as a model for the investigation of human strongyloidiasis caused by S. stercoralis. The latter is a neglected tropical disease in Ecuador where there are no specific plans to mitigate this parasitic illness. Objective: To evaluate the stages of S. venezuelensis in an experimental life cycle using Wistar rats. Materials and methods: Male Wistar rats were used to replicate the natural biological cycle of S. venezuelensis and describe its morphometric characteristics, as well as its parasitic development. Furthermore, the production of eggs per gram of feces was quantified using two diagnostic techniques and assessment of parasite load: Kato-Katz and qPCR. Results: Viable larval stages (L1, L2, L3) could be obtained up to 96 hours through fecal culture. Parthenogenetic females were established in the duodenum on the fifth day postinfection. Fertile eggs were observed in the intestinal tissue and fresh feces where the production peak occurred on the 8th. day post-infection. Unlike Kato-Katz, qPCR detected parasitic DNA on days not typically reported. Conclusions: The larval migration of S. venezuelensis within the murine host in an experimental environment was equivalent to that described in its natural biological cycle. The Kato-Katz quantitative technique showed to be quick and low-cost, but the qPCR had greater diagnostic precision. This experimental life cycle can be used as a tool for the study of strongyloidiasis or other similar nematodiasis. |
format |
Article in Journal/Newspaper |
author |
Jairo Tobar Daniel Ramos Sarmiento Diana Tayupanta Melina Rodríguez Fabián Aguilar |
author_facet |
Jairo Tobar Daniel Ramos Sarmiento Diana Tayupanta Melina Rodríguez Fabián Aguilar |
author_sort |
Jairo Tobar |
title |
Microscopic and molecular evaluation of Strongyloides venezuelensis in an experimental life cycle using Wistar rats |
title_short |
Microscopic and molecular evaluation of Strongyloides venezuelensis in an experimental life cycle using Wistar rats |
title_full |
Microscopic and molecular evaluation of Strongyloides venezuelensis in an experimental life cycle using Wistar rats |
title_fullStr |
Microscopic and molecular evaluation of Strongyloides venezuelensis in an experimental life cycle using Wistar rats |
title_full_unstemmed |
Microscopic and molecular evaluation of Strongyloides venezuelensis in an experimental life cycle using Wistar rats |
title_sort |
microscopic and molecular evaluation of strongyloides venezuelensis in an experimental life cycle using wistar rats |
publisher |
Instituto Nacional de Salud |
publishDate |
2021 |
url |
https://doi.org/10.7705/biomedica.5650 https://doaj.org/article/e7b3ca605daf4d56b28bde0982ecb3bf |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Biomédica: revista del Instituto Nacional de Salud, Vol 41, Iss Supl. 1, Pp 35-46 (2021) |
op_relation |
https://revistabiomedica.org/index.php/biomedica/article/view/5650 https://doaj.org/toc/0120-4157 0120-4157 doi:10.7705/biomedica.5650 https://doaj.org/article/e7b3ca605daf4d56b28bde0982ecb3bf |
op_doi |
https://doi.org/10.7705/biomedica.5650 |
container_title |
Biomédica |
container_volume |
41 |
container_issue |
Supl. 1 |
container_start_page |
35 |
op_container_end_page |
46 |
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1766341554037850112 |