Epigallocatechin-3-gallate exerts antihypertensive effects and improves endothelial function in spontaneously hypertensive rats
Objective: To investigate the effect of epigallocatechin-3-gallate (EGCG) on endothelial dysfunction in spontaneously hypertensive rats (SHR). Methods: Wistar-Kyoto (WKY) rats and SHR were divided into four groups; WKY control, SHR control and SHR treated with EGCG (50 mg/kg/day) or losartan (10 mg/...
Published in: | Asian Pacific Journal of Tropical Biomedicine |
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Wolters Kluwer Medknow Publications
2023
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ftdoajarticles:oai:doaj.org/article:e792308dbd364a07bf7972d2b45eca26 2024-09-09T19:27:04+00:00 Epigallocatechin-3-gallate exerts antihypertensive effects and improves endothelial function in spontaneously hypertensive rats Yucinda YY Khor Siew-Keah Lee M Dharmani Devi Wei Chih Ling 2023-01-01T00:00:00Z https://doi.org/10.4103/2221-1691.380560 https://doaj.org/article/e792308dbd364a07bf7972d2b45eca26 EN eng Wolters Kluwer Medknow Publications http://www.apjtb.org/article.asp?issn=2221-1691;year=2023;volume=13;issue=7;spage=287;epage=295;aulast=Khor https://doaj.org/toc/2221-1691 https://doaj.org/toc/2588-9222 2221-1691 2588-9222 doi:10.4103/2221-1691.380560 https://doaj.org/article/e792308dbd364a07bf7972d2b45eca26 Asian Pacific Journal of Tropical Biomedicine, Vol 13, Iss 7, Pp 287-295 (2023) epigallocatechin-3-gallate vascular protection antihypertension tetrahydrobiopterin cyclic guanosine monophosphate angiotensin type i receptor spontaneously hypertensive rats Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2023 ftdoajarticles https://doi.org/10.4103/2221-1691.380560 2024-08-05T17:49:19Z Objective: To investigate the effect of epigallocatechin-3-gallate (EGCG) on endothelial dysfunction in spontaneously hypertensive rats (SHR). Methods: Wistar-Kyoto (WKY) rats and SHR were divided into four groups; WKY control, SHR control and SHR treated with EGCG (50 mg/kg/day) or losartan (10 mg/kg/day). The treatment was given daily for 4 weeks by oral gavage and the blood pressure was monitored by tail-cuff method every 3 days. Acetylcholine-induced endothelium-dependent relaxations were assessed in isolated phenylephrine-precontracted aortic rings at the end of treatment. The vascular levels of reactive oxygen species, nitric oxide, tetrahydrobiopterin, and cyclic guanosine monophosphate were also measured. Moreover, the expression of angiotensin II type 1 (AT1) receptor protein was determined. Results: The systolic blood pressure was significantly decreased in SHR treated with EGCG. The impaired endothelium-dependent relaxation was significantly improved in aortic ring isolated from the EGCG-treated SHR group. EGCG also significantly increased the levels of nitric oxide, tetrahydrobiopterin, and cyclic guanosine monophosphate, while decreasing the level of reactive oxygen species and the protein expression of AT1 receptor in SHR. Conclusions: EGCG attenuates endothelial dysfunction in SHR by decreasing oxidative stress and increasing vascular nitric oxide bioavailability, which may be modulated partly by inhibition of vascular AT1 receptors. An increase in endothelium-dependent relaxation may contribute to a decrease in blood pressure in hypertensive animals. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 13 7 287 |
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topic |
epigallocatechin-3-gallate vascular protection antihypertension tetrahydrobiopterin cyclic guanosine monophosphate angiotensin type i receptor spontaneously hypertensive rats Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
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epigallocatechin-3-gallate vascular protection antihypertension tetrahydrobiopterin cyclic guanosine monophosphate angiotensin type i receptor spontaneously hypertensive rats Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Yucinda YY Khor Siew-Keah Lee M Dharmani Devi Wei Chih Ling Epigallocatechin-3-gallate exerts antihypertensive effects and improves endothelial function in spontaneously hypertensive rats |
topic_facet |
epigallocatechin-3-gallate vascular protection antihypertension tetrahydrobiopterin cyclic guanosine monophosphate angiotensin type i receptor spontaneously hypertensive rats Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To investigate the effect of epigallocatechin-3-gallate (EGCG) on endothelial dysfunction in spontaneously hypertensive rats (SHR). Methods: Wistar-Kyoto (WKY) rats and SHR were divided into four groups; WKY control, SHR control and SHR treated with EGCG (50 mg/kg/day) or losartan (10 mg/kg/day). The treatment was given daily for 4 weeks by oral gavage and the blood pressure was monitored by tail-cuff method every 3 days. Acetylcholine-induced endothelium-dependent relaxations were assessed in isolated phenylephrine-precontracted aortic rings at the end of treatment. The vascular levels of reactive oxygen species, nitric oxide, tetrahydrobiopterin, and cyclic guanosine monophosphate were also measured. Moreover, the expression of angiotensin II type 1 (AT1) receptor protein was determined. Results: The systolic blood pressure was significantly decreased in SHR treated with EGCG. The impaired endothelium-dependent relaxation was significantly improved in aortic ring isolated from the EGCG-treated SHR group. EGCG also significantly increased the levels of nitric oxide, tetrahydrobiopterin, and cyclic guanosine monophosphate, while decreasing the level of reactive oxygen species and the protein expression of AT1 receptor in SHR. Conclusions: EGCG attenuates endothelial dysfunction in SHR by decreasing oxidative stress and increasing vascular nitric oxide bioavailability, which may be modulated partly by inhibition of vascular AT1 receptors. An increase in endothelium-dependent relaxation may contribute to a decrease in blood pressure in hypertensive animals. |
format |
Article in Journal/Newspaper |
author |
Yucinda YY Khor Siew-Keah Lee M Dharmani Devi Wei Chih Ling |
author_facet |
Yucinda YY Khor Siew-Keah Lee M Dharmani Devi Wei Chih Ling |
author_sort |
Yucinda YY Khor |
title |
Epigallocatechin-3-gallate exerts antihypertensive effects and improves endothelial function in spontaneously hypertensive rats |
title_short |
Epigallocatechin-3-gallate exerts antihypertensive effects and improves endothelial function in spontaneously hypertensive rats |
title_full |
Epigallocatechin-3-gallate exerts antihypertensive effects and improves endothelial function in spontaneously hypertensive rats |
title_fullStr |
Epigallocatechin-3-gallate exerts antihypertensive effects and improves endothelial function in spontaneously hypertensive rats |
title_full_unstemmed |
Epigallocatechin-3-gallate exerts antihypertensive effects and improves endothelial function in spontaneously hypertensive rats |
title_sort |
epigallocatechin-3-gallate exerts antihypertensive effects and improves endothelial function in spontaneously hypertensive rats |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2023 |
url |
https://doi.org/10.4103/2221-1691.380560 https://doaj.org/article/e792308dbd364a07bf7972d2b45eca26 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 13, Iss 7, Pp 287-295 (2023) |
op_relation |
http://www.apjtb.org/article.asp?issn=2221-1691;year=2023;volume=13;issue=7;spage=287;epage=295;aulast=Khor https://doaj.org/toc/2221-1691 https://doaj.org/toc/2588-9222 2221-1691 2588-9222 doi:10.4103/2221-1691.380560 https://doaj.org/article/e792308dbd364a07bf7972d2b45eca26 |
op_doi |
https://doi.org/10.4103/2221-1691.380560 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
13 |
container_issue |
7 |
container_start_page |
287 |
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1809896573055795200 |