Evaluation of immunity against malaria using luciferase-expressing Plasmodium berghei parasites
Abstract Background Measurement of liver stage development is of key interest in malaria biology and vaccine studies. Parasite development in liver cells can be visualized in real-time, both in culture and in live mice, using a transgenic Plasmodium berghei parasite, Pb GFP-Luc con , expressing the...
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ftdoajarticles:oai:doaj.org/article:e7036a5f03bc490ca2f068eb9829b1ae 2023-05-15T15:06:00+02:00 Evaluation of immunity against malaria using luciferase-expressing Plasmodium berghei parasites Ploemen Ivo Behet Marije Nganou-Makamdop Krystelle van Gemert Geert-Jan Bijker Else Hermsen Cornelus Sauerwein Robert 2011-12-01T00:00:00Z https://doi.org/10.1186/1475-2875-10-350 https://doaj.org/article/e7036a5f03bc490ca2f068eb9829b1ae EN eng BMC http://www.malariajournal.com/content/10/1/350 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-10-350 1475-2875 https://doaj.org/article/e7036a5f03bc490ca2f068eb9829b1ae Malaria Journal, Vol 10, Iss 1, p 350 (2011) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2011 ftdoajarticles https://doi.org/10.1186/1475-2875-10-350 2022-12-31T04:19:57Z Abstract Background Measurement of liver stage development is of key interest in malaria biology and vaccine studies. Parasite development in liver cells can be visualized in real-time, both in culture and in live mice, using a transgenic Plasmodium berghei parasite, Pb GFP-Luc con , expressing the bioluminescent reporter luciferase. This study explores the benefit of using these parasites for the evaluation of immunity against malaria, compared to qRT-PCR techniques in vivo and in vitro . Methods Mice were immunized with either radiation attenuated sporozoites (RAS) or wildtype sporozoites under chloroquine prophylaxis (CPS) and challenged with Pb GFP-Luc con. The in vitro transgenic sporozoites neutralization assay (TSNA) was adapted by replacing Pb CS(Pf) parasites for Pb GFP-Luc con parasites. Results Application of Pb GFP-Luc con transgenic parasites provides live quantitative visual information about the relation between parasite liver load and protection. Moreover, fast and reproducible results are obtained by using these parasites in the transgenic sporozoites neutralization assay, measuring functional antibody-mediated immune responses. Conclusions Pb GFP-Luc con parasites are a straightforward and valuable tool for comprehension of the biological and immunological principles underlying protection against malaria. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 10 1 350 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Ploemen Ivo Behet Marije Nganou-Makamdop Krystelle van Gemert Geert-Jan Bijker Else Hermsen Cornelus Sauerwein Robert Evaluation of immunity against malaria using luciferase-expressing Plasmodium berghei parasites |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Measurement of liver stage development is of key interest in malaria biology and vaccine studies. Parasite development in liver cells can be visualized in real-time, both in culture and in live mice, using a transgenic Plasmodium berghei parasite, Pb GFP-Luc con , expressing the bioluminescent reporter luciferase. This study explores the benefit of using these parasites for the evaluation of immunity against malaria, compared to qRT-PCR techniques in vivo and in vitro . Methods Mice were immunized with either radiation attenuated sporozoites (RAS) or wildtype sporozoites under chloroquine prophylaxis (CPS) and challenged with Pb GFP-Luc con. The in vitro transgenic sporozoites neutralization assay (TSNA) was adapted by replacing Pb CS(Pf) parasites for Pb GFP-Luc con parasites. Results Application of Pb GFP-Luc con transgenic parasites provides live quantitative visual information about the relation between parasite liver load and protection. Moreover, fast and reproducible results are obtained by using these parasites in the transgenic sporozoites neutralization assay, measuring functional antibody-mediated immune responses. Conclusions Pb GFP-Luc con parasites are a straightforward and valuable tool for comprehension of the biological and immunological principles underlying protection against malaria. |
format |
Article in Journal/Newspaper |
author |
Ploemen Ivo Behet Marije Nganou-Makamdop Krystelle van Gemert Geert-Jan Bijker Else Hermsen Cornelus Sauerwein Robert |
author_facet |
Ploemen Ivo Behet Marije Nganou-Makamdop Krystelle van Gemert Geert-Jan Bijker Else Hermsen Cornelus Sauerwein Robert |
author_sort |
Ploemen Ivo |
title |
Evaluation of immunity against malaria using luciferase-expressing Plasmodium berghei parasites |
title_short |
Evaluation of immunity against malaria using luciferase-expressing Plasmodium berghei parasites |
title_full |
Evaluation of immunity against malaria using luciferase-expressing Plasmodium berghei parasites |
title_fullStr |
Evaluation of immunity against malaria using luciferase-expressing Plasmodium berghei parasites |
title_full_unstemmed |
Evaluation of immunity against malaria using luciferase-expressing Plasmodium berghei parasites |
title_sort |
evaluation of immunity against malaria using luciferase-expressing plasmodium berghei parasites |
publisher |
BMC |
publishDate |
2011 |
url |
https://doi.org/10.1186/1475-2875-10-350 https://doaj.org/article/e7036a5f03bc490ca2f068eb9829b1ae |
geographic |
Arctic |
geographic_facet |
Arctic |
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Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 10, Iss 1, p 350 (2011) |
op_relation |
http://www.malariajournal.com/content/10/1/350 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-10-350 1475-2875 https://doaj.org/article/e7036a5f03bc490ca2f068eb9829b1ae |
op_doi |
https://doi.org/10.1186/1475-2875-10-350 |
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Malaria Journal |
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10 |
container_issue |
1 |
container_start_page |
350 |
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1766337669204279296 |